Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistance

Detalhes bibliográficos
Autor(a) principal: Salamandane, Acácio
Data de Publicação: 2022
Outros Autores: Alves, Suse, Chambel, Lélia, Malfeito-Ferreira, Manuel, Brito, Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23323
Resumo: The aim of this study was to investigate the pathogenic potential and antibiotic resistance of 59 Escherichia coli isolates from ready-to-eat (RTE) street food (n = 31) and drinking water (n = 28) sold in the city of Maputo, Mozambique. The isolates were characterized by XbaI subtyping analysis via pulsed field gel electrophoresis. Multiplex PCRs were performed targeting five virulence genes (stx, lt, st, astA, and eae) and three groups of antibiotic-resistant genes, namely ß-lactamases (extended-spectrum ß-lactamase and AmpC), tetracycline (tetA, tetB, and tetM) and sulfamethoxazole/ trimethoprim (sul1, sul2, and sul3). The stx virulence gene, encoding the Shiga/Vero (VT) toxin produced by the verotoxin-producing E. coli (VTEC), was identified with similar frequency in isolates from food (5/31) and water (6/28). The highest percentages of resistant isolates from food and water were found for ß-lactams imipenem (35.5 and 39.3%, respectively) and ampicillin (39.3 and 46.4%, respectively). Multidrug resistance was observed in 31.3% of the isolates, being higher in E. coli isolates from water (45.5%) compared to RTE street food isolates (19.2%). Virulence genes were detected in 73% of the multidrug-resistant isolates. Concerning antibiotic-resistant genes, ESBL was the most frequent (57.7%) among -lactamases while tetA was the most frequent (50%) among non- -lactamases
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spelling Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistanceMozambiquestreet fooddrinking waterE. coliPFGEmultidrug resistanceantibiotic resistance genesvirulence genesThe aim of this study was to investigate the pathogenic potential and antibiotic resistance of 59 Escherichia coli isolates from ready-to-eat (RTE) street food (n = 31) and drinking water (n = 28) sold in the city of Maputo, Mozambique. The isolates were characterized by XbaI subtyping analysis via pulsed field gel electrophoresis. Multiplex PCRs were performed targeting five virulence genes (stx, lt, st, astA, and eae) and three groups of antibiotic-resistant genes, namely ß-lactamases (extended-spectrum ß-lactamase and AmpC), tetracycline (tetA, tetB, and tetM) and sulfamethoxazole/ trimethoprim (sul1, sul2, and sul3). The stx virulence gene, encoding the Shiga/Vero (VT) toxin produced by the verotoxin-producing E. coli (VTEC), was identified with similar frequency in isolates from food (5/31) and water (6/28). The highest percentages of resistant isolates from food and water were found for ß-lactams imipenem (35.5 and 39.3%, respectively) and ampicillin (39.3 and 46.4%, respectively). Multidrug resistance was observed in 31.3% of the isolates, being higher in E. coli isolates from water (45.5%) compared to RTE street food isolates (19.2%). Virulence genes were detected in 73% of the multidrug-resistant isolates. Concerning antibiotic-resistant genes, ESBL was the most frequent (57.7%) among -lactamases while tetA was the most frequent (50%) among non- -lactamasesMDPIRepositório da Universidade de LisboaSalamandane, AcácioAlves, SuseChambel, LéliaMalfeito-Ferreira, ManuelBrito, Luísa2022-01-31T11:50:06Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23323engSalamandane, A.; Alves, S.; Chambel, L.; Malfeito-Ferreira, M.; Brito, L. Characterization of Escherichia coli fromWater and Food Sold on the Streets of Maputo: Molecular Typing, Virulence Genes, and Antibiotic Resistance. Appl. Microbiol. 2022, 2, 133–147https:// doi.org/10.3390/applmicrobiol 2010008info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:51Zoai:www.repository.utl.pt:10400.5/23323Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:35.022998Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistance
title Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistance
spellingShingle Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistance
Salamandane, Acácio
Mozambique
street food
drinking water
E. coli
PFGE
multidrug resistance
antibiotic resistance genes
virulence genes
title_short Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistance
title_full Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistance
title_fullStr Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistance
title_full_unstemmed Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistance
title_sort Characterization of Escherichia coli isolates from water and food sold on the streets of Maputo: Molecular typing, virulence genes and antibiotic resistance
author Salamandane, Acácio
author_facet Salamandane, Acácio
Alves, Suse
Chambel, Lélia
Malfeito-Ferreira, Manuel
Brito, Luísa
author_role author
author2 Alves, Suse
Chambel, Lélia
Malfeito-Ferreira, Manuel
Brito, Luísa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Salamandane, Acácio
Alves, Suse
Chambel, Lélia
Malfeito-Ferreira, Manuel
Brito, Luísa
dc.subject.por.fl_str_mv Mozambique
street food
drinking water
E. coli
PFGE
multidrug resistance
antibiotic resistance genes
virulence genes
topic Mozambique
street food
drinking water
E. coli
PFGE
multidrug resistance
antibiotic resistance genes
virulence genes
description The aim of this study was to investigate the pathogenic potential and antibiotic resistance of 59 Escherichia coli isolates from ready-to-eat (RTE) street food (n = 31) and drinking water (n = 28) sold in the city of Maputo, Mozambique. The isolates were characterized by XbaI subtyping analysis via pulsed field gel electrophoresis. Multiplex PCRs were performed targeting five virulence genes (stx, lt, st, astA, and eae) and three groups of antibiotic-resistant genes, namely ß-lactamases (extended-spectrum ß-lactamase and AmpC), tetracycline (tetA, tetB, and tetM) and sulfamethoxazole/ trimethoprim (sul1, sul2, and sul3). The stx virulence gene, encoding the Shiga/Vero (VT) toxin produced by the verotoxin-producing E. coli (VTEC), was identified with similar frequency in isolates from food (5/31) and water (6/28). The highest percentages of resistant isolates from food and water were found for ß-lactams imipenem (35.5 and 39.3%, respectively) and ampicillin (39.3 and 46.4%, respectively). Multidrug resistance was observed in 31.3% of the isolates, being higher in E. coli isolates from water (45.5%) compared to RTE street food isolates (19.2%). Virulence genes were detected in 73% of the multidrug-resistant isolates. Concerning antibiotic-resistant genes, ESBL was the most frequent (57.7%) among -lactamases while tetA was the most frequent (50%) among non- -lactamases
publishDate 2022
dc.date.none.fl_str_mv 2022-01-31T11:50:06Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23323
url http://hdl.handle.net/10400.5/23323
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Salamandane, A.; Alves, S.; Chambel, L.; Malfeito-Ferreira, M.; Brito, L. Characterization of Escherichia coli fromWater and Food Sold on the Streets of Maputo: Molecular Typing, Virulence Genes, and Antibiotic Resistance. Appl. Microbiol. 2022, 2, 133–147
https:// doi.org/10.3390/applmicrobiol 2010008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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