Orotic acid production from crude glycerol by engineered Ashbya gossypii

Detalhes bibliográficos
Autor(a) principal: Silva, Rui
Data de Publicação: 2022
Outros Autores: Aguiar, Tatiana Quinta, Domingues, Lucília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/76422
Resumo: Supplementary data to this article can be found online at https://doi. org/10.1016/j.biteb.2022.100992.
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spelling Orotic acid production from crude glycerol by engineered Ashbya gossypiiDe novo pyrimidine biosynthesisAshbya gossypiiSaccharomyces cerevisiaeURA9Dihydroorotate dehydrogenaseOrotic acidOrotic acid (PubChem CID: 967)Supplementary data to this article can be found online at https://doi. org/10.1016/j.biteb.2022.100992.Orotic acid is an intermediate of the de novo pyrimidine biosynthesis that plays a crucial role in several nutraceutical supplements. In some microorganisms, blockage of this pathway downstream the dihydroorotate dehydrogenase (DHOD) leads to orotic acid accumulation. Here, the Ashbya gossypii Agura3 pyrimidine auxotroph was shown to accumulate and excrete orotic acid (~1.5g/L), and this trait was further explored for the production of orotic acid from raw substrates. Metabolic engineering of this strain combined with culture conditions optimization led to a 3.6-fold increase in orotic acid production from crude glycerol. The mitochondrial DHOD encoded by AgURA9 was shown to be determinant for this phenotype. Heterologous expression of AgURA9 in engineered S. cerevisiae enabled the accumulation and excretion of orotic acid (~0.5g/L). This study demonstrates the potential of A. gossypii for the valorization of crude glycerol to orotic acid and discloses the molecular determinants for its biosynthesis in fungi.This work was carried out at the Biomass and Bioenergy Research Infrastructure (BBRI)-LISBOA-01-0145-FEDER-022059, supported by Operational Programme for Competitiveness and Internationalization (PORTUGAL2020), by Lisbon Portugal Regional Operational Programme (Lisboa 2020), and by North Portugal Regional Operational Program (Norte 2020) under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and has been supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020, project ESSEntial (PTDC/BII-BTI/1858/2021) and the Doctoral Program in Applied and Environmental Microbiology (PhD grant PD/BD/113812/2015 to R. Silva).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoSilva, RuiAguiar, Tatiana QuintaDomingues, Lucília2022-022022-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/76422engSilva, Rui; Aguiar, Tatiana Q.; Domingues, Lucília, Orotic acid production from crude glycerol by engineered Ashbya gossypii. Bioresource Technology Reports, 17(100992), 20222589-014X10.1016/j.biteb.2022.100992100992https://www.sciencedirect.com/journal/bioresource-technology-reportsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:35:21Zoai:repositorium.sdum.uminho.pt:1822/76422Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:31:11.651437Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Orotic acid production from crude glycerol by engineered Ashbya gossypii
title Orotic acid production from crude glycerol by engineered Ashbya gossypii
spellingShingle Orotic acid production from crude glycerol by engineered Ashbya gossypii
Silva, Rui
De novo pyrimidine biosynthesis
Ashbya gossypii
Saccharomyces cerevisiae
URA9
Dihydroorotate dehydrogenase
Orotic acid
Orotic acid (PubChem CID: 967)
title_short Orotic acid production from crude glycerol by engineered Ashbya gossypii
title_full Orotic acid production from crude glycerol by engineered Ashbya gossypii
title_fullStr Orotic acid production from crude glycerol by engineered Ashbya gossypii
title_full_unstemmed Orotic acid production from crude glycerol by engineered Ashbya gossypii
title_sort Orotic acid production from crude glycerol by engineered Ashbya gossypii
author Silva, Rui
author_facet Silva, Rui
Aguiar, Tatiana Quinta
Domingues, Lucília
author_role author
author2 Aguiar, Tatiana Quinta
Domingues, Lucília
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Rui
Aguiar, Tatiana Quinta
Domingues, Lucília
dc.subject.por.fl_str_mv De novo pyrimidine biosynthesis
Ashbya gossypii
Saccharomyces cerevisiae
URA9
Dihydroorotate dehydrogenase
Orotic acid
Orotic acid (PubChem CID: 967)
topic De novo pyrimidine biosynthesis
Ashbya gossypii
Saccharomyces cerevisiae
URA9
Dihydroorotate dehydrogenase
Orotic acid
Orotic acid (PubChem CID: 967)
description Supplementary data to this article can be found online at https://doi. org/10.1016/j.biteb.2022.100992.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
2022-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/76422
url http://hdl.handle.net/1822/76422
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, Rui; Aguiar, Tatiana Q.; Domingues, Lucília, Orotic acid production from crude glycerol by engineered Ashbya gossypii. Bioresource Technology Reports, 17(100992), 2022
2589-014X
10.1016/j.biteb.2022.100992
100992
https://www.sciencedirect.com/journal/bioresource-technology-reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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