Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/31812 |
Resumo: | Antimicrobial resistance (AMR) has become a global concern as many bacterial species have developed resistance to commonly prescribed antibiotics, making them ineffective to treatments. One type of antibiotics, gallium(III) compounds, stands out as possible candidates due to their unique “Trojan horse” mechanism to tackle bacterial growth, by substituting iron(III) in the metabolic cycles of bacteria. In this study, we tested three polysaccharides (carboxymethyl cellulose (CMC), alginate, and pectin) as the binding and delivery agent for gallium on three bacteria (Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus) with a potential bioresponsive delivery mode. Two types of analysis on bacterial growth (minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC)) were carried out while iron(III)-loaded polysaccharide samples were also tested for comparison. The results suggested that gallium showed an improved inhibitory activity on bacterial growth, in particular gallium(III)-loaded carboxymethyl cellulose (Ga-CMC) sample showing an inhibiting effect on growth for all three tested bacteria. At the MIC for all three bacteria, Ga-CMC showed no cytotoxicity effect on human dermal neonatal fibroblasts (HDNF). Therefore, these bioresponsive gallium(III) polysaccharide compounds show significant potential to be developed as the next-generation antibacterial agents with controlled release capability. |
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Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitroGalliumCarbohydratesAntibacterialAntibioticsAnaerobicAntimicrobial resistance (AMR) has become a global concern as many bacterial species have developed resistance to commonly prescribed antibiotics, making them ineffective to treatments. One type of antibiotics, gallium(III) compounds, stands out as possible candidates due to their unique “Trojan horse” mechanism to tackle bacterial growth, by substituting iron(III) in the metabolic cycles of bacteria. In this study, we tested three polysaccharides (carboxymethyl cellulose (CMC), alginate, and pectin) as the binding and delivery agent for gallium on three bacteria (Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus) with a potential bioresponsive delivery mode. Two types of analysis on bacterial growth (minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC)) were carried out while iron(III)-loaded polysaccharide samples were also tested for comparison. The results suggested that gallium showed an improved inhibitory activity on bacterial growth, in particular gallium(III)-loaded carboxymethyl cellulose (Ga-CMC) sample showing an inhibiting effect on growth for all three tested bacteria. At the MIC for all three bacteria, Ga-CMC showed no cytotoxicity effect on human dermal neonatal fibroblasts (HDNF). Therefore, these bioresponsive gallium(III) polysaccharide compounds show significant potential to be developed as the next-generation antibacterial agents with controlled release capability.American Chemical SocietyVeritati - Repositório Institucional da Universidade Católica PortuguesaBest, Mark G.Cunha-Reis, CassildaGanin, Alexey Y.Sousa, AurelianaJohnston, JennaOliveira, Ana L.Smith, David G. E.Yiu, Humphrey H. P.Cooper, Ian R.2021-01-28T16:28:07Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/31812engBest, M. G., Cunha-Reis, C., Ganin, A. Y., Sousa, A., Johnston, J., Oliveira, A. L., ... Cooper, I. R. (2020). Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro. ACS Applied Bio Materials2576-642210.1021/acsabm.0c0081185094585663000604607600032info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-17T01:35:49Zoai:repositorio.ucp.pt:10400.14/31812Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:25:39.946482Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro |
title |
Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro |
spellingShingle |
Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro Best, Mark G. Gallium Carbohydrates Antibacterial Antibiotics Anaerobic |
title_short |
Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro |
title_full |
Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro |
title_fullStr |
Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro |
title_full_unstemmed |
Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro |
title_sort |
Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro |
author |
Best, Mark G. |
author_facet |
Best, Mark G. Cunha-Reis, Cassilda Ganin, Alexey Y. Sousa, Aureliana Johnston, Jenna Oliveira, Ana L. Smith, David G. E. Yiu, Humphrey H. P. Cooper, Ian R. |
author_role |
author |
author2 |
Cunha-Reis, Cassilda Ganin, Alexey Y. Sousa, Aureliana Johnston, Jenna Oliveira, Ana L. Smith, David G. E. Yiu, Humphrey H. P. Cooper, Ian R. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Best, Mark G. Cunha-Reis, Cassilda Ganin, Alexey Y. Sousa, Aureliana Johnston, Jenna Oliveira, Ana L. Smith, David G. E. Yiu, Humphrey H. P. Cooper, Ian R. |
dc.subject.por.fl_str_mv |
Gallium Carbohydrates Antibacterial Antibiotics Anaerobic |
topic |
Gallium Carbohydrates Antibacterial Antibiotics Anaerobic |
description |
Antimicrobial resistance (AMR) has become a global concern as many bacterial species have developed resistance to commonly prescribed antibiotics, making them ineffective to treatments. One type of antibiotics, gallium(III) compounds, stands out as possible candidates due to their unique “Trojan horse” mechanism to tackle bacterial growth, by substituting iron(III) in the metabolic cycles of bacteria. In this study, we tested three polysaccharides (carboxymethyl cellulose (CMC), alginate, and pectin) as the binding and delivery agent for gallium on three bacteria (Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus) with a potential bioresponsive delivery mode. Two types of analysis on bacterial growth (minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC)) were carried out while iron(III)-loaded polysaccharide samples were also tested for comparison. The results suggested that gallium showed an improved inhibitory activity on bacterial growth, in particular gallium(III)-loaded carboxymethyl cellulose (Ga-CMC) sample showing an inhibiting effect on growth for all three tested bacteria. At the MIC for all three bacteria, Ga-CMC showed no cytotoxicity effect on human dermal neonatal fibroblasts (HDNF). Therefore, these bioresponsive gallium(III) polysaccharide compounds show significant potential to be developed as the next-generation antibacterial agents with controlled release capability. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-01-28T16:28:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/31812 |
url |
http://hdl.handle.net/10400.14/31812 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Best, M. G., Cunha-Reis, C., Ganin, A. Y., Sousa, A., Johnston, J., Oliveira, A. L., ... Cooper, I. R. (2020). Antimicrobial properties of gallium (III)-and iron (III)-loaded polysaccharides affecting the growth of escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa, in vitro. ACS Applied Bio Materials 2576-6422 10.1021/acsabm.0c00811 85094585663 000604607600032 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131972510941184 |