Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/139406 |
Resumo: | Funding Information: This research received funding from the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAUPUB/32587/2017). The following financial grants are also acknowledged: Projects UIDB/00009/2020 and UIDP/00009/2020 (ToxOmics); Projects UIDB/04138/2020 and UIDP/04138/2020 (iMed.Ulisboa) and L. Gonçalves Principal Researcher grant (CEECIND/03143/2017); FEDER and POR Lisboa2020 through the COMPETE 2020 POCI and PORL; POCI-01-0145-FEDER-007688 (UIDB/50025/2020- 2023); NanoCell2SEC (PTDC/CTM-REF/30529/2017); CIEPQPF (UIDB/00102/2020); European Topology Interdisciplinary Action (EUTOPIA CA17139); Inpactus Project—Innovative Products and Technologies from Eucalyptus (Project Nº 21874, funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 Nº246/AXIS II/2017). Nádia Vital holds an FCT/MCTES PhD Scholarship grant (2020.07168.BD). |
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Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubescell uptakegenotoxicityin vitro cytotoxicitymicro/nanocellulosesmicronucleus assayreactive oxygen speciesChemical Engineering(all)Materials Science(all)SDG 7 - Affordable and Clean EnergyFunding Information: This research received funding from the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAUPUB/32587/2017). The following financial grants are also acknowledged: Projects UIDB/00009/2020 and UIDP/00009/2020 (ToxOmics); Projects UIDB/04138/2020 and UIDP/04138/2020 (iMed.Ulisboa) and L. Gonçalves Principal Researcher grant (CEECIND/03143/2017); FEDER and POR Lisboa2020 through the COMPETE 2020 POCI and PORL; POCI-01-0145-FEDER-007688 (UIDB/50025/2020- 2023); NanoCell2SEC (PTDC/CTM-REF/30529/2017); CIEPQPF (UIDB/00102/2020); European Topology Interdisciplinary Action (EUTOPIA CA17139); Inpactus Project—Innovative Products and Technologies from Eucalyptus (Project Nº 21874, funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 Nº246/AXIS II/2017). Nádia Vital holds an FCT/MCTES PhD Scholarship grant (2020.07168.BD).Cellulose micro/nanomaterials (CMNM), comprising cellulose microfibrils (CMF), nanofibrils (CNF), and nanocrystals (CNC), are being recognized as promising bio-nanomaterials due to their natural and renewable source, attractive properties, and potential for applications with industrial and economical value. Thus, it is crucial to investigate their potential toxicity before starting their production at a larger scale. The present study aimed at evaluating the cell internalization and in vitro cytotoxicity and genotoxicity of CMNM as compared to two multi-walled carbon nanotubes (MWCNT), NM-401 and NM-402, in A549 cells. The exposure to all studied NM, with the exception of CNC, resulted in evident cellular uptake, as analyzed by transmission electron microscopy. However, none of the CMNM induced cytotoxic effects, in contrast to the cytotoxicity observed for the MWCNT. Furthermore, no genotoxicity was observed for CNF, CNC, and NM-402 (cytokinesis-block micronucleus assay), while CMF and NM-401 were able to significantly raise micronucleus frequency. Only NM-402 was able to induce ROS formation, although it did not induce micronuclei. Thus, it is unlikely that the observed CMF and NM-401 genotoxicity is mediated by oxidative DNA damage. More studies targeting other genotoxicity endpoints and cellular and molecular events are underway to allow for a more comprehensive safety assessment of these nanocelluloses.Centre for Toxicogenomics and Human Health (ToxOmics)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)DCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)Faculdade de Ciências e Tecnologia (FCT)RUNPinto, FátimaLourenço, Ana FilipaPedrosa, Jorge F.S.Gonçalves, LídiaVentura, CéliaVital, NádiaBettencourt, AnaFernandes, Susete N.da Rosa, Rafaela R.Godinho, Maria HelenaLouro, HenriquetaFerreira, Paulo J.T.Silva, Maria João2022-06-03T22:31:11Z2022-04-222022-04-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/139406eng2079-4991PURE: 43678941https://doi.org/10.3390/nano12091432info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:16:46Zoai:run.unl.pt:10362/139406Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:49:26.212897Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes |
| title |
Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes |
| spellingShingle |
Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes Pinto, Fátima cell uptake genotoxicity in vitro cytotoxicity micro/nanocelluloses micronucleus assay reactive oxygen species Chemical Engineering(all) Materials Science(all) SDG 7 - Affordable and Clean Energy |
| title_short |
Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes |
| title_full |
Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes |
| title_fullStr |
Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes |
| title_full_unstemmed |
Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes |
| title_sort |
Analysis of the In Vitro Toxicity of Nanocelluloses in Human Lung Cells as Compared to Multi-Walled Carbon Nanotubes |
| author |
Pinto, Fátima |
| author_facet |
Pinto, Fátima Lourenço, Ana Filipa Pedrosa, Jorge F.S. Gonçalves, Lídia Ventura, Célia Vital, Nádia Bettencourt, Ana Fernandes, Susete N. da Rosa, Rafaela R. Godinho, Maria Helena Louro, Henriqueta Ferreira, Paulo J.T. Silva, Maria João |
| author_role |
author |
| author2 |
Lourenço, Ana Filipa Pedrosa, Jorge F.S. Gonçalves, Lídia Ventura, Célia Vital, Nádia Bettencourt, Ana Fernandes, Susete N. da Rosa, Rafaela R. Godinho, Maria Helena Louro, Henriqueta Ferreira, Paulo J.T. Silva, Maria João |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Centre for Toxicogenomics and Human Health (ToxOmics) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) DCM - Departamento de Ciência dos Materiais CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) Faculdade de Ciências e Tecnologia (FCT) RUN |
| dc.contributor.author.fl_str_mv |
Pinto, Fátima Lourenço, Ana Filipa Pedrosa, Jorge F.S. Gonçalves, Lídia Ventura, Célia Vital, Nádia Bettencourt, Ana Fernandes, Susete N. da Rosa, Rafaela R. Godinho, Maria Helena Louro, Henriqueta Ferreira, Paulo J.T. Silva, Maria João |
| dc.subject.por.fl_str_mv |
cell uptake genotoxicity in vitro cytotoxicity micro/nanocelluloses micronucleus assay reactive oxygen species Chemical Engineering(all) Materials Science(all) SDG 7 - Affordable and Clean Energy |
| topic |
cell uptake genotoxicity in vitro cytotoxicity micro/nanocelluloses micronucleus assay reactive oxygen species Chemical Engineering(all) Materials Science(all) SDG 7 - Affordable and Clean Energy |
| description |
Funding Information: This research received funding from the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAUPUB/32587/2017). The following financial grants are also acknowledged: Projects UIDB/00009/2020 and UIDP/00009/2020 (ToxOmics); Projects UIDB/04138/2020 and UIDP/04138/2020 (iMed.Ulisboa) and L. Gonçalves Principal Researcher grant (CEECIND/03143/2017); FEDER and POR Lisboa2020 through the COMPETE 2020 POCI and PORL; POCI-01-0145-FEDER-007688 (UIDB/50025/2020- 2023); NanoCell2SEC (PTDC/CTM-REF/30529/2017); CIEPQPF (UIDB/00102/2020); European Topology Interdisciplinary Action (EUTOPIA CA17139); Inpactus Project—Innovative Products and Technologies from Eucalyptus (Project Nº 21874, funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 Nº246/AXIS II/2017). Nádia Vital holds an FCT/MCTES PhD Scholarship grant (2020.07168.BD). |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-06-03T22:31:11Z 2022-04-22 2022-04-22T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/139406 |
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http://hdl.handle.net/10362/139406 |
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eng |
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eng |
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2079-4991 PURE: 43678941 https://doi.org/10.3390/nano12091432 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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