Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/8358 |
Resumo: | This article belongs to the Section Nanotoxicology and Nanopharmacology. |
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Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 CellsNanofibrillated CelluloseNanocrystalline CelluloseNanotoxicologyCytotoxicityEnvironmental GenotoxicityMicronucleus AssayGenotoxicityMicronucleus AssayGenotoxicidade AmbientalThis article belongs to the Section Nanotoxicology and Nanopharmacology.Background: Nanocellulose is an innovative engineered nanomaterial with an enormous potential for use in a wide array of industrial and biomedical applications and with fast growing economic value. The expanding production of nanocellulose is leading to an increased human exposure, raising concerns about their potential health effects. This study was aimed at assessing the potential toxic and genotoxic effects of different nanocelluloses in two mammalian cell lines; Methods: Two micro/nanocelluloses, produced with a TEMPO oxidation pre-treatment (CNFs) and an enzymatic pre-treatment (CMFs), and cellulose nanocrystals (CNCs) were tested in osteoblastic-like human cells (MG-63) and Chinese hamster lung fibroblasts (V79) using the MTT and clonogenic assays to analyse cytotoxicity, and the micronucleus assay to test genotoxicity; Results: cytotoxicity was observed by the clonogenic assay in V79 cells, particularly for CNCs, but not by the MTT assay; CNF induced micronuclei in both cell lines and nucleoplasmic bridges in MG-63 cells; CMF and CNC induced micronuclei and nucleoplasmic bridges in MG-63 cells, but not in V79 cells; Conclusions: All nanocelluloses revealed cytotoxicity and genotoxicity, although at different concentrations, that may be related to their physicochemical differences and availability for cell uptake, and to differences in the DNA damage response of the cell model.The research was funded by the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017). The following funding is also acknowledged: POCI-01-0145-FEDER-007688 (UIDB/50025/2020-2023); NanoCell2SEC (PTDC/CTM-REF/30529/2017); ToxOmics (UIDP/00009/2020; UIDB/00009/2020); CIEPQPF (UIDB/00102/2020); European Topology Interdisciplinary Action (EUTOPIA CA17139); Inpactus Project—innovative products and technologies from eucalyptus (Project N.◦ 21874 funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 n ◦ 246/AXIS II/2017)MDPIRepositório Científico do Instituto Nacional de SaúdeVentura, CéliaMarques, CatarinaCadete, JoãoVilar, MadalenaPedrosa, Jorge F.S.Pinto, FátimaFernandes, Susete Nogueirada Rosa, Rafaela RauppGodinho, Maria HelenaFerreira, Paulo J.T.Louro, HenriquetaSilva, Maria João2022-11-29T10:08:43Z2022-04-212022-04-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8358engJ Xenobiot. 2022 Apr 21;12(2):91-108. doi: 10.3390/jox120200092039-471310.3390/jox12020009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:29Zoai:repositorio.insa.pt:10400.18/8358Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:56.221503Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| spellingShingle |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells Ventura, Célia Nanofibrillated Cellulose Nanocrystalline Cellulose Nanotoxicology Cytotoxicity Environmental Genotoxicity Micronucleus Assay Genotoxicity Micronucleus Assay Genotoxicidade Ambiental |
| title_short |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title_full |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title_fullStr |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title_full_unstemmed |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| title_sort |
Genotoxicity of Three Micro/Nanocelluloses with Different Physicochemical Characteristics in MG-63 and V79 Cells |
| author |
Ventura, Célia |
| author_facet |
Ventura, Célia Marques, Catarina Cadete, João Vilar, Madalena Pedrosa, Jorge F.S. Pinto, Fátima Fernandes, Susete Nogueira da Rosa, Rafaela Raupp Godinho, Maria Helena Ferreira, Paulo J.T. Louro, Henriqueta Silva, Maria João |
| author_role |
author |
| author2 |
Marques, Catarina Cadete, João Vilar, Madalena Pedrosa, Jorge F.S. Pinto, Fátima Fernandes, Susete Nogueira da Rosa, Rafaela Raupp Godinho, Maria Helena Ferreira, Paulo J.T. Louro, Henriqueta Silva, Maria João |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Ventura, Célia Marques, Catarina Cadete, João Vilar, Madalena Pedrosa, Jorge F.S. Pinto, Fátima Fernandes, Susete Nogueira da Rosa, Rafaela Raupp Godinho, Maria Helena Ferreira, Paulo J.T. Louro, Henriqueta Silva, Maria João |
| dc.subject.por.fl_str_mv |
Nanofibrillated Cellulose Nanocrystalline Cellulose Nanotoxicology Cytotoxicity Environmental Genotoxicity Micronucleus Assay Genotoxicity Micronucleus Assay Genotoxicidade Ambiental |
| topic |
Nanofibrillated Cellulose Nanocrystalline Cellulose Nanotoxicology Cytotoxicity Environmental Genotoxicity Micronucleus Assay Genotoxicity Micronucleus Assay Genotoxicidade Ambiental |
| description |
This article belongs to the Section Nanotoxicology and Nanopharmacology. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-11-29T10:08:43Z 2022-04-21 2022-04-21T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/8358 |
| url |
http://hdl.handle.net/10400.18/8358 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
J Xenobiot. 2022 Apr 21;12(2):91-108. doi: 10.3390/jox12020009 2039-4713 10.3390/jox12020009 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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