Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases

Detalhes bibliográficos
Autor(a) principal: Valdivieso González, David
Data de Publicação: 2023
Outros Autores: Makowski, Marcin, Lillo, M. Pilar, Cao-García, Francisco J., Melo, Manuel N., Almendro-Vedia, Víctor G., López-Montero, Iván
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/165650
Resumo: Funding Information: D.V.G. and M.M. contributed equally to this work and are co‐first authors. I.L.‐M. and M.P.L. acknowledge financial support from the Spanish Ministry of Science, Innovation and Universities through the grant PGC2018‐097903‐B‐I00. This work was also supported by the TECNOLOGÍAS 2018 program funded by the Regional Government of Madrid (Grant S2018/BAA‐4403 SINOXPHOS‐CM) and PID2021‐125024NB‐C22 (To I.L.‐M.). DVG thanks the Regional Government of Madrid for an assistant researcher contract (CT103/19/PEJ‐2019‐AI/IND‐13687). M.M. acknowledges Fundação para a Ciência e a Tecnologia's (FCT) fellowship SPRH/BD/128290/2017. M.N.M. acknowledges financial support from FCT for fellowship CEECIND/04124/2017 and project MOSTMICRO‐ITQB, with references UIDB/04612/2020 and UIDP/04612/2020. F.J.C.‐G. was supported by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund through grant RTI2018‐095802‐B‐I00. V.G.A.‐V. thanks the Regional Government of Madrid, Universidad Complutense de Madrid and Fundación Complutense for computational resources (by Grant PR27/21 030). I.L.‐M. thanks Dr. Frédéric Pincet and Dr. Paolo Natale for fruitful discussions. Publisher Copyright: © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
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spelling Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP SynthasesE.coliFF ATP synthasegiant vesicleslipid nanotubesmicromanipulationMedicine (miscellaneous)Chemical Engineering(all)Materials Science(all)Biochemistry, Genetics and Molecular Biology (miscellaneous)Engineering(all)Physics and Astronomy(all)Funding Information: D.V.G. and M.M. contributed equally to this work and are co‐first authors. I.L.‐M. and M.P.L. acknowledge financial support from the Spanish Ministry of Science, Innovation and Universities through the grant PGC2018‐097903‐B‐I00. This work was also supported by the TECNOLOGÍAS 2018 program funded by the Regional Government of Madrid (Grant S2018/BAA‐4403 SINOXPHOS‐CM) and PID2021‐125024NB‐C22 (To I.L.‐M.). DVG thanks the Regional Government of Madrid for an assistant researcher contract (CT103/19/PEJ‐2019‐AI/IND‐13687). M.M. acknowledges Fundação para a Ciência e a Tecnologia's (FCT) fellowship SPRH/BD/128290/2017. M.N.M. acknowledges financial support from FCT for fellowship CEECIND/04124/2017 and project MOSTMICRO‐ITQB, with references UIDB/04612/2020 and UIDP/04612/2020. F.J.C.‐G. was supported by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund through grant RTI2018‐095802‐B‐I00. V.G.A.‐V. thanks the Regional Government of Madrid, Universidad Complutense de Madrid and Fundación Complutense for computational resources (by Grant PR27/21 030). I.L.‐M. thanks Dr. Frédéric Pincet and Dr. Paolo Natale for fruitful discussions. Publisher Copyright: © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.ATP synthases are proteins that catalyse the formation of ATP through the rotatory movement of their membrane-spanning subunit. In mitochondria, ATP synthases are found to arrange as dimers at the high-curved edges of cristae. Here, a direct link is explored between the rotatory movement of ATP synthases and their preference for curved membranes. An active curvature sorting of ATP synthases in lipid nanotubes pulled from giant vesicles is found. Coarse-grained simulations confirm the curvature-seeking behaviour of rotating ATP synthases, promoting reversible and frequent protein-protein contacts. The formation of transient protein dimers relies on the membrane-mediated attractive interaction of the order of 1.5 kBT produced by a hydrophobic mismatch upon protein rotation. Transient dimers are sustained by a conic-like arrangement characterized by a wedge angle of θ ≈ 50°, producing a dynamic coupling between protein shape and membrane curvature. The results suggest a new role of the rotational movement of ATP synthases for their dynamic self-assembly in biological membranes.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNValdivieso González, DavidMakowski, MarcinLillo, M. PilarCao-García, Francisco J.Melo, Manuel N.Almendro-Vedia, Víctor G.López-Montero, Iván2024-03-31T23:34:07Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/165650eng2198-3844PURE: 75015065https://doi.org/10.1002/advs.202301606info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:20:04Zoai:run.unl.pt:10362/165650Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:20:04Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases
title Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases
spellingShingle Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases
Valdivieso González, David
E.coli
FF ATP synthase
giant vesicles
lipid nanotubes
micromanipulation
Medicine (miscellaneous)
Chemical Engineering(all)
Materials Science(all)
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Engineering(all)
Physics and Astronomy(all)
title_short Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases
title_full Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases
title_fullStr Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases
title_full_unstemmed Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases
title_sort Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases
author Valdivieso González, David
author_facet Valdivieso González, David
Makowski, Marcin
Lillo, M. Pilar
Cao-García, Francisco J.
Melo, Manuel N.
Almendro-Vedia, Víctor G.
López-Montero, Iván
author_role author
author2 Makowski, Marcin
Lillo, M. Pilar
Cao-García, Francisco J.
Melo, Manuel N.
Almendro-Vedia, Víctor G.
López-Montero, Iván
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Valdivieso González, David
Makowski, Marcin
Lillo, M. Pilar
Cao-García, Francisco J.
Melo, Manuel N.
Almendro-Vedia, Víctor G.
López-Montero, Iván
dc.subject.por.fl_str_mv E.coli
FF ATP synthase
giant vesicles
lipid nanotubes
micromanipulation
Medicine (miscellaneous)
Chemical Engineering(all)
Materials Science(all)
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Engineering(all)
Physics and Astronomy(all)
topic E.coli
FF ATP synthase
giant vesicles
lipid nanotubes
micromanipulation
Medicine (miscellaneous)
Chemical Engineering(all)
Materials Science(all)
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Engineering(all)
Physics and Astronomy(all)
description Funding Information: D.V.G. and M.M. contributed equally to this work and are co‐first authors. I.L.‐M. and M.P.L. acknowledge financial support from the Spanish Ministry of Science, Innovation and Universities through the grant PGC2018‐097903‐B‐I00. This work was also supported by the TECNOLOGÍAS 2018 program funded by the Regional Government of Madrid (Grant S2018/BAA‐4403 SINOXPHOS‐CM) and PID2021‐125024NB‐C22 (To I.L.‐M.). DVG thanks the Regional Government of Madrid for an assistant researcher contract (CT103/19/PEJ‐2019‐AI/IND‐13687). M.M. acknowledges Fundação para a Ciência e a Tecnologia's (FCT) fellowship SPRH/BD/128290/2017. M.N.M. acknowledges financial support from FCT for fellowship CEECIND/04124/2017 and project MOSTMICRO‐ITQB, with references UIDB/04612/2020 and UIDP/04612/2020. F.J.C.‐G. was supported by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund through grant RTI2018‐095802‐B‐I00. V.G.A.‐V. thanks the Regional Government of Madrid, Universidad Complutense de Madrid and Fundación Complutense for computational resources (by Grant PR27/21 030). I.L.‐M. thanks Dr. Frédéric Pincet and Dr. Paolo Natale for fruitful discussions. Publisher Copyright: © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01T00:00:00Z
2024-03-31T23:34:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/165650
url http://hdl.handle.net/10362/165650
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2198-3844
PURE: 75015065
https://doi.org/10.1002/advs.202301606
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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