Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/165650 |
Resumo: | Funding Information: D.V.G. and M.M. contributed equally to this work and are co‐first authors. I.L.‐M. and M.P.L. acknowledge financial support from the Spanish Ministry of Science, Innovation and Universities through the grant PGC2018‐097903‐B‐I00. This work was also supported by the TECNOLOGÍAS 2018 program funded by the Regional Government of Madrid (Grant S2018/BAA‐4403 SINOXPHOS‐CM) and PID2021‐125024NB‐C22 (To I.L.‐M.). DVG thanks the Regional Government of Madrid for an assistant researcher contract (CT103/19/PEJ‐2019‐AI/IND‐13687). M.M. acknowledges Fundação para a Ciência e a Tecnologia's (FCT) fellowship SPRH/BD/128290/2017. M.N.M. acknowledges financial support from FCT for fellowship CEECIND/04124/2017 and project MOSTMICRO‐ITQB, with references UIDB/04612/2020 and UIDP/04612/2020. F.J.C.‐G. was supported by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund through grant RTI2018‐095802‐B‐I00. V.G.A.‐V. thanks the Regional Government of Madrid, Universidad Complutense de Madrid and Fundación Complutense for computational resources (by Grant PR27/21 030). I.L.‐M. thanks Dr. Frédéric Pincet and Dr. Paolo Natale for fruitful discussions. Publisher Copyright: © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH. |
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Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP SynthasesE.coliFF ATP synthasegiant vesicleslipid nanotubesmicromanipulationMedicine (miscellaneous)Chemical Engineering(all)Materials Science(all)Biochemistry, Genetics and Molecular Biology (miscellaneous)Engineering(all)Physics and Astronomy(all)Funding Information: D.V.G. and M.M. contributed equally to this work and are co‐first authors. I.L.‐M. and M.P.L. acknowledge financial support from the Spanish Ministry of Science, Innovation and Universities through the grant PGC2018‐097903‐B‐I00. This work was also supported by the TECNOLOGÍAS 2018 program funded by the Regional Government of Madrid (Grant S2018/BAA‐4403 SINOXPHOS‐CM) and PID2021‐125024NB‐C22 (To I.L.‐M.). DVG thanks the Regional Government of Madrid for an assistant researcher contract (CT103/19/PEJ‐2019‐AI/IND‐13687). M.M. acknowledges Fundação para a Ciência e a Tecnologia's (FCT) fellowship SPRH/BD/128290/2017. M.N.M. acknowledges financial support from FCT for fellowship CEECIND/04124/2017 and project MOSTMICRO‐ITQB, with references UIDB/04612/2020 and UIDP/04612/2020. F.J.C.‐G. was supported by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund through grant RTI2018‐095802‐B‐I00. V.G.A.‐V. thanks the Regional Government of Madrid, Universidad Complutense de Madrid and Fundación Complutense for computational resources (by Grant PR27/21 030). I.L.‐M. thanks Dr. Frédéric Pincet and Dr. Paolo Natale for fruitful discussions. Publisher Copyright: © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.ATP synthases are proteins that catalyse the formation of ATP through the rotatory movement of their membrane-spanning subunit. In mitochondria, ATP synthases are found to arrange as dimers at the high-curved edges of cristae. Here, a direct link is explored between the rotatory movement of ATP synthases and their preference for curved membranes. An active curvature sorting of ATP synthases in lipid nanotubes pulled from giant vesicles is found. Coarse-grained simulations confirm the curvature-seeking behaviour of rotating ATP synthases, promoting reversible and frequent protein-protein contacts. The formation of transient protein dimers relies on the membrane-mediated attractive interaction of the order of 1.5 kBT produced by a hydrophobic mismatch upon protein rotation. Transient dimers are sustained by a conic-like arrangement characterized by a wedge angle of θ ≈ 50°, producing a dynamic coupling between protein shape and membrane curvature. The results suggest a new role of the rotational movement of ATP synthases for their dynamic self-assembly in biological membranes.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNValdivieso González, DavidMakowski, MarcinLillo, M. PilarCao-García, Francisco J.Melo, Manuel N.Almendro-Vedia, Víctor G.López-Montero, Iván2024-03-31T23:34:07Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/165650eng2198-3844PURE: 75015065https://doi.org/10.1002/advs.202301606info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:20:04Zoai:run.unl.pt:10362/165650Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:20:04Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases |
title |
Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases |
spellingShingle |
Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases Valdivieso González, David E.coli FF ATP synthase giant vesicles lipid nanotubes micromanipulation Medicine (miscellaneous) Chemical Engineering(all) Materials Science(all) Biochemistry, Genetics and Molecular Biology (miscellaneous) Engineering(all) Physics and Astronomy(all) |
title_short |
Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases |
title_full |
Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases |
title_fullStr |
Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases |
title_full_unstemmed |
Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases |
title_sort |
Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases |
author |
Valdivieso González, David |
author_facet |
Valdivieso González, David Makowski, Marcin Lillo, M. Pilar Cao-García, Francisco J. Melo, Manuel N. Almendro-Vedia, Víctor G. López-Montero, Iván |
author_role |
author |
author2 |
Makowski, Marcin Lillo, M. Pilar Cao-García, Francisco J. Melo, Manuel N. Almendro-Vedia, Víctor G. López-Montero, Iván |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
Valdivieso González, David Makowski, Marcin Lillo, M. Pilar Cao-García, Francisco J. Melo, Manuel N. Almendro-Vedia, Víctor G. López-Montero, Iván |
dc.subject.por.fl_str_mv |
E.coli FF ATP synthase giant vesicles lipid nanotubes micromanipulation Medicine (miscellaneous) Chemical Engineering(all) Materials Science(all) Biochemistry, Genetics and Molecular Biology (miscellaneous) Engineering(all) Physics and Astronomy(all) |
topic |
E.coli FF ATP synthase giant vesicles lipid nanotubes micromanipulation Medicine (miscellaneous) Chemical Engineering(all) Materials Science(all) Biochemistry, Genetics and Molecular Biology (miscellaneous) Engineering(all) Physics and Astronomy(all) |
description |
Funding Information: D.V.G. and M.M. contributed equally to this work and are co‐first authors. I.L.‐M. and M.P.L. acknowledge financial support from the Spanish Ministry of Science, Innovation and Universities through the grant PGC2018‐097903‐B‐I00. This work was also supported by the TECNOLOGÍAS 2018 program funded by the Regional Government of Madrid (Grant S2018/BAA‐4403 SINOXPHOS‐CM) and PID2021‐125024NB‐C22 (To I.L.‐M.). DVG thanks the Regional Government of Madrid for an assistant researcher contract (CT103/19/PEJ‐2019‐AI/IND‐13687). M.M. acknowledges Fundação para a Ciência e a Tecnologia's (FCT) fellowship SPRH/BD/128290/2017. M.N.M. acknowledges financial support from FCT for fellowship CEECIND/04124/2017 and project MOSTMICRO‐ITQB, with references UIDB/04612/2020 and UIDP/04612/2020. F.J.C.‐G. was supported by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund through grant RTI2018‐095802‐B‐I00. V.G.A.‐V. thanks the Regional Government of Madrid, Universidad Complutense de Madrid and Fundación Complutense for computational resources (by Grant PR27/21 030). I.L.‐M. thanks Dr. Frédéric Pincet and Dr. Paolo Natale for fruitful discussions. Publisher Copyright: © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 2023-01-01T00:00:00Z 2024-03-31T23:34:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/165650 |
url |
http://hdl.handle.net/10362/165650 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2198-3844 PURE: 75015065 https://doi.org/10.1002/advs.202301606 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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