Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration

Detalhes bibliográficos
Autor(a) principal: Santo, Vitor E.
Data de Publicação: 2015
Outros Autores: Ratanavaraporn, Juthamas, Sato, Keisuke, Gomes, Manuela E., Mano, J. F., Reis, R. L., Tabata, Yasuhiko
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/36612
Resumo: To direct precursor cells toward the osteoblastic lineage, by using an intracellular nanocarrier releasing dexamethasone. Materials & methods: Biodegradable gelatinbased micelles entrapped dexamethasone (dex-micelles). Internalization efficiency and biocompatibility of dex-micelles and their potency for in vitro osteogenic differentiation and in vivo bone regeneration were assessed. Results: Dex-micelles were internalized by rat bone marrow mesenchymal stem cells and demonstrated a pH-responsive release profile and an enhancement of 2D and 3D in vitro osteogenic differentiation. In vivo implantation of gelatin scaffolds seeded with rat bone marrow mesenchymal stem cells precultured for 24 h with dex-micelles promoted a significant enhancement of de novo bone formation in a rat ulna defect, in a dose-dependent manner. Conclusion: The proposed intracellular delivery system is a powerful tool to promote bone regeneration.
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spelling Cell engineering by the internalization of bioinstructive micelles for enhanced bone regenerationBone regenerationIntracellular deliveryMesenchymal stem cellMicelleNanoparticleTissue engineeringScience & TechnologyTo direct precursor cells toward the osteoblastic lineage, by using an intracellular nanocarrier releasing dexamethasone. Materials & methods: Biodegradable gelatinbased micelles entrapped dexamethasone (dex-micelles). Internalization efficiency and biocompatibility of dex-micelles and their potency for in vitro osteogenic differentiation and in vivo bone regeneration were assessed. Results: Dex-micelles were internalized by rat bone marrow mesenchymal stem cells and demonstrated a pH-responsive release profile and an enhancement of 2D and 3D in vitro osteogenic differentiation. In vivo implantation of gelatin scaffolds seeded with rat bone marrow mesenchymal stem cells precultured for 24 h with dex-micelles promoted a significant enhancement of de novo bone formation in a rat ulna defect, in a dose-dependent manner. Conclusion: The proposed intracellular delivery system is a powerful tool to promote bone regeneration.The authors thank Fundacao para a Ciencia e Tecnologia and Japan Society for the Promotion of Science (JSPS) for VE Santo's PhD grant (SFRH/BD/39486/2007) and J Ratanavaraporn's post-doc fellowship, respectively. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.Future MedicineUniversidade do MinhoSanto, Vitor E.Ratanavaraporn, JuthamasSato, KeisukeGomes, Manuela E.Mano, J. F.Reis, R. L.Tabata, Yasuhiko2015-082015-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/36612engSanto V. E., Ratanavaraporn J., Sato K., Gomes M. E., Mano J. F., Reis R. L., Tabata Y. Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration, Nanomedicine, Vol. 10, Issue 11, pp. 1707-1721, doi:10.2217/nnm.15.11 , 20151743-588910.2217/nnm.15.1126080695http://www.futuremedicine.com/doi/abs/10.2217/nnm.15.11info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:23:30Zoai:repositorium.sdum.uminho.pt:1822/36612Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:17:14.676897Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration
title Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration
spellingShingle Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration
Santo, Vitor E.
Bone regeneration
Intracellular delivery
Mesenchymal stem cell
Micelle
Nanoparticle
Tissue engineering
Science & Technology
title_short Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration
title_full Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration
title_fullStr Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration
title_full_unstemmed Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration
title_sort Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration
author Santo, Vitor E.
author_facet Santo, Vitor E.
Ratanavaraporn, Juthamas
Sato, Keisuke
Gomes, Manuela E.
Mano, J. F.
Reis, R. L.
Tabata, Yasuhiko
author_role author
author2 Ratanavaraporn, Juthamas
Sato, Keisuke
Gomes, Manuela E.
Mano, J. F.
Reis, R. L.
Tabata, Yasuhiko
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santo, Vitor E.
Ratanavaraporn, Juthamas
Sato, Keisuke
Gomes, Manuela E.
Mano, J. F.
Reis, R. L.
Tabata, Yasuhiko
dc.subject.por.fl_str_mv Bone regeneration
Intracellular delivery
Mesenchymal stem cell
Micelle
Nanoparticle
Tissue engineering
Science & Technology
topic Bone regeneration
Intracellular delivery
Mesenchymal stem cell
Micelle
Nanoparticle
Tissue engineering
Science & Technology
description To direct precursor cells toward the osteoblastic lineage, by using an intracellular nanocarrier releasing dexamethasone. Materials & methods: Biodegradable gelatinbased micelles entrapped dexamethasone (dex-micelles). Internalization efficiency and biocompatibility of dex-micelles and their potency for in vitro osteogenic differentiation and in vivo bone regeneration were assessed. Results: Dex-micelles were internalized by rat bone marrow mesenchymal stem cells and demonstrated a pH-responsive release profile and an enhancement of 2D and 3D in vitro osteogenic differentiation. In vivo implantation of gelatin scaffolds seeded with rat bone marrow mesenchymal stem cells precultured for 24 h with dex-micelles promoted a significant enhancement of de novo bone formation in a rat ulna defect, in a dose-dependent manner. Conclusion: The proposed intracellular delivery system is a powerful tool to promote bone regeneration.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
2015-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/36612
url http://hdl.handle.net/1822/36612
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santo V. E., Ratanavaraporn J., Sato K., Gomes M. E., Mano J. F., Reis R. L., Tabata Y. Cell engineering by the internalization of bioinstructive micelles for enhanced bone regeneration, Nanomedicine, Vol. 10, Issue 11, pp. 1707-1721, doi:10.2217/nnm.15.11 , 2015
1743-5889
10.2217/nnm.15.11
26080695
http://www.futuremedicine.com/doi/abs/10.2217/nnm.15.11
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Future Medicine
publisher.none.fl_str_mv Future Medicine
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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