Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/37611 |
Resumo: | Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission. |
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7160 |
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Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspectiveCattleCowsStaphylococcus aureusTransmissionStaphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission.Veritati - Repositório Institucional da Universidade Católica PortuguesaSilva, VanessaCorreia, SusanaRocha, JaquelineManaia, Célia M.Silva, AdrianaGarcía-Díez, JuanPereira, José EduardoSemedo-Lemsaddek, TeresaIgrejas, GilbertoPoeta, Patrícia2022-05-17T16:06:39Z2022-052022-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/37611eng2076-260710.3390/microorganisms1005094185129212727PMC914482035630384000804277200001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T12:37:25Zoai:repositorio.ucp.pt:10400.14/37611Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T12:37:25Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective |
title |
Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective |
spellingShingle |
Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective Silva, Vanessa Cattle Cows Staphylococcus aureus Transmission |
title_short |
Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective |
title_full |
Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective |
title_fullStr |
Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective |
title_full_unstemmed |
Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective |
title_sort |
Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective |
author |
Silva, Vanessa |
author_facet |
Silva, Vanessa Correia, Susana Rocha, Jaqueline Manaia, Célia M. Silva, Adriana García-Díez, Juan Pereira, José Eduardo Semedo-Lemsaddek, Teresa Igrejas, Gilberto Poeta, Patrícia |
author_role |
author |
author2 |
Correia, Susana Rocha, Jaqueline Manaia, Célia M. Silva, Adriana García-Díez, Juan Pereira, José Eduardo Semedo-Lemsaddek, Teresa Igrejas, Gilberto Poeta, Patrícia |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Silva, Vanessa Correia, Susana Rocha, Jaqueline Manaia, Célia M. Silva, Adriana García-Díez, Juan Pereira, José Eduardo Semedo-Lemsaddek, Teresa Igrejas, Gilberto Poeta, Patrícia |
dc.subject.por.fl_str_mv |
Cattle Cows Staphylococcus aureus Transmission |
topic |
Cattle Cows Staphylococcus aureus Transmission |
description |
Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-17T16:06:39Z 2022-05 2022-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/37611 |
url |
http://hdl.handle.net/10400.14/37611 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2076-2607 10.3390/microorganisms10050941 85129212727 PMC9144820 35630384 000804277200001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817547047728840704 |