Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective

Detalhes bibliográficos
Autor(a) principal: Silva, Vanessa
Data de Publicação: 2022
Outros Autores: Correia, Susana, Rocha, Jaqueline, Manaia, Célia M., Silva, Adriana, García-Díez, Juan, Pereira, José Eduardo, Semedo-Lemsaddek, Teresa, Igrejas, Gilberto, Poeta, Patrícia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/37611
Resumo: Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission.
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spelling Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspectiveCattleCowsStaphylococcus aureusTransmissionStaphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission.Veritati - Repositório Institucional da Universidade Católica PortuguesaSilva, VanessaCorreia, SusanaRocha, JaquelineManaia, Célia M.Silva, AdrianaGarcía-Díez, JuanPereira, José EduardoSemedo-Lemsaddek, TeresaIgrejas, GilbertoPoeta, Patrícia2022-05-17T16:06:39Z2022-052022-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/37611eng2076-260710.3390/microorganisms1005094185129212727PMC914482035630384000804277200001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T12:37:25Zoai:repositorio.ucp.pt:10400.14/37611Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T12:37:25Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective
title Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective
spellingShingle Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective
Silva, Vanessa
Cattle
Cows
Staphylococcus aureus
Transmission
title_short Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective
title_full Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective
title_fullStr Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective
title_full_unstemmed Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective
title_sort Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective
author Silva, Vanessa
author_facet Silva, Vanessa
Correia, Susana
Rocha, Jaqueline
Manaia, Célia M.
Silva, Adriana
García-Díez, Juan
Pereira, José Eduardo
Semedo-Lemsaddek, Teresa
Igrejas, Gilberto
Poeta, Patrícia
author_role author
author2 Correia, Susana
Rocha, Jaqueline
Manaia, Célia M.
Silva, Adriana
García-Díez, Juan
Pereira, José Eduardo
Semedo-Lemsaddek, Teresa
Igrejas, Gilberto
Poeta, Patrícia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Silva, Vanessa
Correia, Susana
Rocha, Jaqueline
Manaia, Célia M.
Silva, Adriana
García-Díez, Juan
Pereira, José Eduardo
Semedo-Lemsaddek, Teresa
Igrejas, Gilberto
Poeta, Patrícia
dc.subject.por.fl_str_mv Cattle
Cows
Staphylococcus aureus
Transmission
topic Cattle
Cows
Staphylococcus aureus
Transmission
description Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-17T16:06:39Z
2022-05
2022-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/37611
url http://hdl.handle.net/10400.14/37611
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-2607
10.3390/microorganisms10050941
85129212727
PMC9144820
35630384
000804277200001
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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