Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings

Detalhes bibliográficos
Autor(a) principal: Silva, Vanessa
Data de Publicação: 2022
Outros Autores: Correia, Susana, Rocha, Jaqueline, Manaia, Célia M., Silva, Adriana, García-Díez, Juan, Pereira, José Eduardo, Semedo-Lemsaddek, Teresa, Igrejas, Gilberto, Poeta, Patrícia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/151079
Resumo: This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with references NORTE-01-0145-FEDER-030101. projects UIDB/CVT/00772/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their SurroundingsA Cross-Sectional Study from the One Health PerspectivecattlecowsStaphylococcus aureustransmissionMicrobiologyMicrobiology (medical)VirologyThis work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with references NORTE-01-0145-FEDER-030101. projects UIDB/CVT/00772/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission.LAQV@REQUIMTERUNSilva, VanessaCorreia, SusanaRocha, JaquelineManaia, Célia M.Silva, AdrianaGarcía-Díez, JuanPereira, José EduardoSemedo-Lemsaddek, TeresaIgrejas, GilbertoPoeta, Patrícia2023-03-22T22:29:02Z2022-04-302022-04-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/151079eng2076-2607PURE: 56618001https://doi.org/10.3390/microorganisms10050941info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:33:31Zoai:run.unl.pt:10362/151079Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:27.136163Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings
A Cross-Sectional Study from the One Health Perspective
title Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings
spellingShingle Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings
Silva, Vanessa
cattle
cows
Staphylococcus aureus
transmission
Microbiology
Microbiology (medical)
Virology
title_short Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings
title_full Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings
title_fullStr Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings
title_full_unstemmed Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings
title_sort Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings
author Silva, Vanessa
author_facet Silva, Vanessa
Correia, Susana
Rocha, Jaqueline
Manaia, Célia M.
Silva, Adriana
García-Díez, Juan
Pereira, José Eduardo
Semedo-Lemsaddek, Teresa
Igrejas, Gilberto
Poeta, Patrícia
author_role author
author2 Correia, Susana
Rocha, Jaqueline
Manaia, Célia M.
Silva, Adriana
García-Díez, Juan
Pereira, José Eduardo
Semedo-Lemsaddek, Teresa
Igrejas, Gilberto
Poeta, Patrícia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
RUN
dc.contributor.author.fl_str_mv Silva, Vanessa
Correia, Susana
Rocha, Jaqueline
Manaia, Célia M.
Silva, Adriana
García-Díez, Juan
Pereira, José Eduardo
Semedo-Lemsaddek, Teresa
Igrejas, Gilberto
Poeta, Patrícia
dc.subject.por.fl_str_mv cattle
cows
Staphylococcus aureus
transmission
Microbiology
Microbiology (medical)
Virology
topic cattle
cows
Staphylococcus aureus
transmission
Microbiology
Microbiology (medical)
Virology
description This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with references NORTE-01-0145-FEDER-030101. projects UIDB/CVT/00772/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-30
2022-04-30T00:00:00Z
2023-03-22T22:29:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/151079
url http://hdl.handle.net/10362/151079
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-2607
PURE: 56618001
https://doi.org/10.3390/microorganisms10050941
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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