Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/44525 |
Resumo: | Due to the actual emphasis in revalorization of agro-industrial wastes and cost reduction of enzymes production, this work aimed the optimization of lipase production from different oil cakes (OCs) produced in Brazil, by solid-state fermentation using Aspergillus ibericus MUM 03.49 and the application of the produced lipase in hydrolysis and esterification reactions. Results presented showed higher lipase production using palm kernel oil cake (PKOC), yielding 127 ± 17 U/g. Through SSF optimization, using palm kernel oil cake (PKOC) mixed with sesame oil cake (SOC) in a ratio of 0.45 g/g PKOC per g total substrate at 57% moisture content, a production of 460 ± 38 U/g of lipase per mass of dry substrate was obtained after 6 days of fermentation. The obtained lipase was used in hydrolysis reactions, where it was observed higher production in short-chain triacylglycerols (TAGs) substrates. Also, it was applied in esterification reactions, where the formation of butyl decanoate using 5% (w/v) of biocatalyst was the most efficient. SSF of PKOC and SOC was a low cost competitive process to obtain A. ibericus lipase that can be used in aroma esters production, with application in the food industry. |
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Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactionsPalm kernel oil cakesesame oil cakesolid-state fermentationA. ibericuslipase productionaroma esters productionScience & TechnologyDue to the actual emphasis in revalorization of agro-industrial wastes and cost reduction of enzymes production, this work aimed the optimization of lipase production from different oil cakes (OCs) produced in Brazil, by solid-state fermentation using Aspergillus ibericus MUM 03.49 and the application of the produced lipase in hydrolysis and esterification reactions. Results presented showed higher lipase production using palm kernel oil cake (PKOC), yielding 127 ± 17 U/g. Through SSF optimization, using palm kernel oil cake (PKOC) mixed with sesame oil cake (SOC) in a ratio of 0.45 g/g PKOC per g total substrate at 57% moisture content, a production of 460 ± 38 U/g of lipase per mass of dry substrate was obtained after 6 days of fermentation. The obtained lipase was used in hydrolysis reactions, where it was observed higher production in short-chain triacylglycerols (TAGs) substrates. Also, it was applied in esterification reactions, where the formation of butyl decanoate using 5% (w/v) of biocatalyst was the most efficient. SSF of PKOC and SOC was a low cost competitive process to obtain A. ibericus lipase that can be used in aroma esters production, with application in the food industry.Felisbela Oliveira acknowledges the financial support from FAPERJ N° 43/2013—Programa de Apoio ao Doutorado-Sanduíche Reverso—2013, from Rio de Janeiro, Brazil and from the Portuguese Foundation for Science and Technology (FCT), through the grant SFRH/BD/87953/2012. José Manuel Salgado was supported by grant CEB/N2020 – INV/01/2016 from Project “BIOTECNORTE - Underpinning Biotechnology to foster the north of Portugal bioeconomy” (NORTE-01-0145-FEDER-000004). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Carlos E. Souza and Verônica R.O.L. Peclat thank the financial support given by CNPq and CAPES from Brazil, respectively. Bernardo D. Ribeiro and Maria A.Z. Coelho thank the financial support given by CNPq and FAPERJ from Brazil.Institution of Chemical Engineers (IChemE)Universidade do MinhoOliveira, Felisbela Maria AraújoSouza, Carlos E.Peclat, Verônica R. O. L.Salgado, José ManuelRibeiro, Bernardo D.Coelho, Maria A. Z.Venâncio, ArmandoBelo, Isabel2017-032017-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44525engOliveira, Felisbela Maria Araújo; Souza, Carlos E.; Peclat, Verônica R. O. L.; Salgado, José Manuel; Ribeiro, Bernardo D.; Coelho, Maria A. Z.; Venâncio, Armando; Belo, Isabel, Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions. Food and Bioproducts Processing, 102, 268-277, 20170960-30851744-357110.1016/j.fbp.2017.01.007http://www.elsevier.com/locate/issn/09603085info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:54:54Zoai:repositorium.sdum.uminho.pt:1822/44525Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:44:22.869853Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions |
title |
Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions |
spellingShingle |
Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions Oliveira, Felisbela Maria Araújo Palm kernel oil cake sesame oil cake solid-state fermentation A. ibericus lipase production aroma esters production Science & Technology |
title_short |
Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions |
title_full |
Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions |
title_fullStr |
Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions |
title_full_unstemmed |
Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions |
title_sort |
Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions |
author |
Oliveira, Felisbela Maria Araújo |
author_facet |
Oliveira, Felisbela Maria Araújo Souza, Carlos E. Peclat, Verônica R. O. L. Salgado, José Manuel Ribeiro, Bernardo D. Coelho, Maria A. Z. Venâncio, Armando Belo, Isabel |
author_role |
author |
author2 |
Souza, Carlos E. Peclat, Verônica R. O. L. Salgado, José Manuel Ribeiro, Bernardo D. Coelho, Maria A. Z. Venâncio, Armando Belo, Isabel |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, Felisbela Maria Araújo Souza, Carlos E. Peclat, Verônica R. O. L. Salgado, José Manuel Ribeiro, Bernardo D. Coelho, Maria A. Z. Venâncio, Armando Belo, Isabel |
dc.subject.por.fl_str_mv |
Palm kernel oil cake sesame oil cake solid-state fermentation A. ibericus lipase production aroma esters production Science & Technology |
topic |
Palm kernel oil cake sesame oil cake solid-state fermentation A. ibericus lipase production aroma esters production Science & Technology |
description |
Due to the actual emphasis in revalorization of agro-industrial wastes and cost reduction of enzymes production, this work aimed the optimization of lipase production from different oil cakes (OCs) produced in Brazil, by solid-state fermentation using Aspergillus ibericus MUM 03.49 and the application of the produced lipase in hydrolysis and esterification reactions. Results presented showed higher lipase production using palm kernel oil cake (PKOC), yielding 127 ± 17 U/g. Through SSF optimization, using palm kernel oil cake (PKOC) mixed with sesame oil cake (SOC) in a ratio of 0.45 g/g PKOC per g total substrate at 57% moisture content, a production of 460 ± 38 U/g of lipase per mass of dry substrate was obtained after 6 days of fermentation. The obtained lipase was used in hydrolysis reactions, where it was observed higher production in short-chain triacylglycerols (TAGs) substrates. Also, it was applied in esterification reactions, where the formation of butyl decanoate using 5% (w/v) of biocatalyst was the most efficient. SSF of PKOC and SOC was a low cost competitive process to obtain A. ibericus lipase that can be used in aroma esters production, with application in the food industry. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03 2017-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/44525 |
url |
http://hdl.handle.net/1822/44525 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira, Felisbela Maria Araújo; Souza, Carlos E.; Peclat, Verônica R. O. L.; Salgado, José Manuel; Ribeiro, Bernardo D.; Coelho, Maria A. Z.; Venâncio, Armando; Belo, Isabel, Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions. Food and Bioproducts Processing, 102, 268-277, 2017 0960-3085 1744-3571 10.1016/j.fbp.2017.01.007 http://www.elsevier.com/locate/issn/09603085 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institution of Chemical Engineers (IChemE) |
publisher.none.fl_str_mv |
Institution of Chemical Engineers (IChemE) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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