Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/2565 |
Resumo: | Pseudomonas putida ATCC 17514 was used as a model strain to investigate the characteristics of bacterial growth in the presence of solid fluorene and phenanthrene. Despite the lower water-solubility of phenanthrene, P. putida degraded this polycyclic aromatic hydrocarbon (PAH) at a maximum observed rate of 1.4 ± 0.1 mg Lˉ¹ hˉ¹, higher than the apparent degradation rate of fluorene, 0.8 ± 0.07 mg Lˉ¹ hˉ¹. The role of physiological processes on the biodegradation of these PAHs was analyzed and two different uptake strategies were identified. Zeta potential measurements revealed that phenanthrene-grown cells were slightly more negatively charged (–57.5 ± 4.7 mV) than fluorene-grown cells (–51.6 ± 4.9 mV), but much more negatively charged than glucose-grown cells (–26.8 ± 3.3 mV), suggesting that the PAH substrate induced modifications on the physical properties of bacterial surfaces. Furthermore, protein-to-exopolysaccharide ratios detected during bacterial growth on phenanthrene were typical of biofilms developed under physicochemical stress conditions, caused by the presence of sparingly water-soluble chemicals as the sole carbon and energy source for growth, the maximum value for TP/EPS during growth on phenanthrene (1.9) being lower than the one obtained with fluorene (5.5). Finally, confocal laser microscopy observations using a gfp-labeled derivative strain revealed that, in the presence of phenanthrene, P. putida::gfp cells formed a biofilm on accessible crystal surfaces, whereas in the presence of fluorene the strain grew randomly between the crystal clusters. The results showed that P. putida was able to overcome the lower aqueous solubility of phenanthrene by adhering to the solid PAH throughout the production of extracellular polymeric substances, thus promoting the availability and uptake of such a hydrophobic compound. |
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Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspectsFluorenePhenanthreneBiodegradationCLSMGFPScience & TechnologyPseudomonas putida ATCC 17514 was used as a model strain to investigate the characteristics of bacterial growth in the presence of solid fluorene and phenanthrene. Despite the lower water-solubility of phenanthrene, P. putida degraded this polycyclic aromatic hydrocarbon (PAH) at a maximum observed rate of 1.4 ± 0.1 mg Lˉ¹ hˉ¹, higher than the apparent degradation rate of fluorene, 0.8 ± 0.07 mg Lˉ¹ hˉ¹. The role of physiological processes on the biodegradation of these PAHs was analyzed and two different uptake strategies were identified. Zeta potential measurements revealed that phenanthrene-grown cells were slightly more negatively charged (–57.5 ± 4.7 mV) than fluorene-grown cells (–51.6 ± 4.9 mV), but much more negatively charged than glucose-grown cells (–26.8 ± 3.3 mV), suggesting that the PAH substrate induced modifications on the physical properties of bacterial surfaces. Furthermore, protein-to-exopolysaccharide ratios detected during bacterial growth on phenanthrene were typical of biofilms developed under physicochemical stress conditions, caused by the presence of sparingly water-soluble chemicals as the sole carbon and energy source for growth, the maximum value for TP/EPS during growth on phenanthrene (1.9) being lower than the one obtained with fluorene (5.5). Finally, confocal laser microscopy observations using a gfp-labeled derivative strain revealed that, in the presence of phenanthrene, P. putida::gfp cells formed a biofilm on accessible crystal surfaces, whereas in the presence of fluorene the strain grew randomly between the crystal clusters. The results showed that P. putida was able to overcome the lower aqueous solubility of phenanthrene by adhering to the solid PAH throughout the production of extracellular polymeric substances, thus promoting the availability and uptake of such a hydrophobic compound.Fundação para a Ciência e a Tecnologia (FCT) – PRAXISXXI/BD/15944/98.EC Biotech program - BIO4-CT97-2015.John Wiley and SonsUniversidade do MinhoRodrigues, A. C.Wuertz, StefanBrito, A. G.Melo, L. F.20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2565eng"Biotechnology and bioengineering". ISSN 0006-3592. 90:3 (2005) 281-289.0006-359210.1002/bit.2037715800860http://eu.wiley.com/WileyCDA/http://www3.interscience.wiley.com/cgi-bin/fulltext/110435733/PDFSTARTinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:56:07Zoai:repositorium.sdum.uminho.pt:1822/2565Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:45:43.511625Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects |
title |
Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects |
spellingShingle |
Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects Rodrigues, A. C. Fluorene Phenanthrene Biodegradation CLSM GFP Science & Technology |
title_short |
Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects |
title_full |
Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects |
title_fullStr |
Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects |
title_full_unstemmed |
Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects |
title_sort |
Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects |
author |
Rodrigues, A. C. |
author_facet |
Rodrigues, A. C. Wuertz, Stefan Brito, A. G. Melo, L. F. |
author_role |
author |
author2 |
Wuertz, Stefan Brito, A. G. Melo, L. F. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Rodrigues, A. C. Wuertz, Stefan Brito, A. G. Melo, L. F. |
dc.subject.por.fl_str_mv |
Fluorene Phenanthrene Biodegradation CLSM GFP Science & Technology |
topic |
Fluorene Phenanthrene Biodegradation CLSM GFP Science & Technology |
description |
Pseudomonas putida ATCC 17514 was used as a model strain to investigate the characteristics of bacterial growth in the presence of solid fluorene and phenanthrene. Despite the lower water-solubility of phenanthrene, P. putida degraded this polycyclic aromatic hydrocarbon (PAH) at a maximum observed rate of 1.4 ± 0.1 mg Lˉ¹ hˉ¹, higher than the apparent degradation rate of fluorene, 0.8 ± 0.07 mg Lˉ¹ hˉ¹. The role of physiological processes on the biodegradation of these PAHs was analyzed and two different uptake strategies were identified. Zeta potential measurements revealed that phenanthrene-grown cells were slightly more negatively charged (–57.5 ± 4.7 mV) than fluorene-grown cells (–51.6 ± 4.9 mV), but much more negatively charged than glucose-grown cells (–26.8 ± 3.3 mV), suggesting that the PAH substrate induced modifications on the physical properties of bacterial surfaces. Furthermore, protein-to-exopolysaccharide ratios detected during bacterial growth on phenanthrene were typical of biofilms developed under physicochemical stress conditions, caused by the presence of sparingly water-soluble chemicals as the sole carbon and energy source for growth, the maximum value for TP/EPS during growth on phenanthrene (1.9) being lower than the one obtained with fluorene (5.5). Finally, confocal laser microscopy observations using a gfp-labeled derivative strain revealed that, in the presence of phenanthrene, P. putida::gfp cells formed a biofilm on accessible crystal surfaces, whereas in the presence of fluorene the strain grew randomly between the crystal clusters. The results showed that P. putida was able to overcome the lower aqueous solubility of phenanthrene by adhering to the solid PAH throughout the production of extracellular polymeric substances, thus promoting the availability and uptake of such a hydrophobic compound. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 2005-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/2565 |
url |
http://hdl.handle.net/1822/2565 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Biotechnology and bioengineering". ISSN 0006-3592. 90:3 (2005) 281-289. 0006-3592 10.1002/bit.20377 15800860 http://eu.wiley.com/WileyCDA/ http://www3.interscience.wiley.com/cgi-bin/fulltext/110435733/PDFSTART |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley and Sons |
publisher.none.fl_str_mv |
John Wiley and Sons |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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