Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrin

Detalhes bibliográficos
Autor(a) principal: Ramos, Margarida
Data de Publicação: 2010
Outros Autores: Salústio, Paulo, Serralheiro, Luísa, Frazão, Fátima, Marques, Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/3863
Resumo: The original publication is available at www.springerlink.com. A publicação original está disponível em www.springerlink.com
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spelling Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrinOmeprazoleHydroxypropyl-b-cyclodextrinInclusion complexStabilityEnzymatic activityThe original publication is available at www.springerlink.com. A publicação original está disponível em www.springerlink.comOmeprazole (OME) exhibits low stability to light, heat and humidity. In stress conditions OME stability should improve under inclusion complex form with hydroxypropyl-b-cyclodextrin (HPbCD). Stability of OME, its physical mixture (PM) with HPbCD and OME:HPbCD inclusion complex was assessed during 60 days. The inclusion complexes were prepared by kneading and freezedrying techniques and characterized by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR). A molecular modelling was also held to predict the most probable tridimensional conformation of inclusion complex OME:HPbCD. The inhibitory activity of free and complexed OME on selected enzymes, namely, papain (protease model of the proton pump) and acetylcholinesterase (enzyme present in cholinergic neurons and also involved in Alzheimer’s disease) was compared. The results obtained show that HPbCD do not protect against OME degradation, in any prepared powder, in the presence of light, heat and humidity. This may indicate that the reactive group of OME is not included in the HPbCD cavity. This fact is supported by molecular modelling data, which demonstrated that 2-pyridylmethyl group of OME is not included into the cyclodextrin cavity. In relation to enzymatic assays it was observed that free OME and OME in the binary systems showed identical inhibitory activity on papain and acethylcolinesterase, concluding that HPbCD do not affect OME activity on these two enzymes.SpringerRepositório da Universidade de LisboaRamos, MargaridaSalústio, PauloSerralheiro, LuísaFrazão, FátimaMarques, Helena2011-08-04T12:40:26Z2010-10-282010-10-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/3863engJournal of Inclusion Phenomena and Macrocyclic Chemistry, 2011, 70(3-4), 407-4140923-0750http://dx.doi.org/10.1007/s10847-010-9884-3info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:44:09Zoai:repositorio.ul.pt:10451/3863Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:29:28.870203Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrin
title Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrin
spellingShingle Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrin
Ramos, Margarida
Omeprazole
Hydroxypropyl-b-cyclodextrin
Inclusion complex
Stability
Enzymatic activity
title_short Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrin
title_full Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrin
title_fullStr Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrin
title_full_unstemmed Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrin
title_sort Stability and enzymatic studies with omeprazole: hydroxypropyl-β-cyclodextrin
author Ramos, Margarida
author_facet Ramos, Margarida
Salústio, Paulo
Serralheiro, Luísa
Frazão, Fátima
Marques, Helena
author_role author
author2 Salústio, Paulo
Serralheiro, Luísa
Frazão, Fátima
Marques, Helena
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Ramos, Margarida
Salústio, Paulo
Serralheiro, Luísa
Frazão, Fátima
Marques, Helena
dc.subject.por.fl_str_mv Omeprazole
Hydroxypropyl-b-cyclodextrin
Inclusion complex
Stability
Enzymatic activity
topic Omeprazole
Hydroxypropyl-b-cyclodextrin
Inclusion complex
Stability
Enzymatic activity
description The original publication is available at www.springerlink.com. A publicação original está disponível em www.springerlink.com
publishDate 2010
dc.date.none.fl_str_mv 2010-10-28
2010-10-28T00:00:00Z
2011-08-04T12:40:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/3863
url http://hdl.handle.net/10451/3863
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2011, 70(3-4), 407-414
0923-0750
http://dx.doi.org/10.1007/s10847-010-9884-3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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