Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex

Detalhes bibliográficos
Autor(a) principal: Burga-Sánchez, Jonny
Data de Publicação: 2020
Outros Autores: Ferreira, Luiz Eduardo Nunes, Volpato, Maria Cristina, Cabeça, Luis Fernando, Braga, Mario, Fraceto, Leonardo Fernandes [UNESP], de Paula, Eneida, Groppo, Francisco Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00210-020-01879-1
http://hdl.handle.net/11449/200456
Resumo: Articaine (ATC) is one of the most widely used local anesthetics in dentistry. Despite its safety, local toxicity has been reported. This study aimed to develop an ATC-2- hydroxypropyl-β-cyclodextrin inclusion complex (ATC HPβCD) and to assess its toxicity in vitro. The inclusion complex was performed by solubilization, followed by a fluorimetric and job plot assay to determine the complex stoichiometry. Scanning electron microscopy, DOSY- 1 H-NMR, differential scanning calorimetry (DSC), and sustained release kinetics were used to confirm the inclusion complex formation. In vitro cytotoxicity was analyzed by MTT assay and immunofluorescence in HGF cells. Fluorimetric and job plot assay determined the inclusion complex stoichiometry (ATC:HPβCD = 1:1) and complex formation time (400 min), as indicated by a strong host/guest interaction (Ka = 117.8 M − 1), complexed fraction (f = 41.4%), and different ATC and ATC HPβCD melting points (172 °C e 235 °C, respectively). The mean of cell viability was 31.87% and 63.17% for 20-mM ATC and 20-mM ATC HPβCD, respectively. Moreover, remarkable cell toxicity was observed with free ATC by immunofluorescence. These results indicate the ATC HPβCD complex could be used to improve the safety of ATC. Further research are needed to establish the anesthetic safety and effectiveness in vivo.
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spelling Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complexArticaineCell viabilityHuman gingival fibroblastHydroxypropyl-β-cyclodextrinArticaine (ATC) is one of the most widely used local anesthetics in dentistry. Despite its safety, local toxicity has been reported. This study aimed to develop an ATC-2- hydroxypropyl-β-cyclodextrin inclusion complex (ATC HPβCD) and to assess its toxicity in vitro. The inclusion complex was performed by solubilization, followed by a fluorimetric and job plot assay to determine the complex stoichiometry. Scanning electron microscopy, DOSY- 1 H-NMR, differential scanning calorimetry (DSC), and sustained release kinetics were used to confirm the inclusion complex formation. In vitro cytotoxicity was analyzed by MTT assay and immunofluorescence in HGF cells. Fluorimetric and job plot assay determined the inclusion complex stoichiometry (ATC:HPβCD = 1:1) and complex formation time (400 min), as indicated by a strong host/guest interaction (Ka = 117.8 M − 1), complexed fraction (f = 41.4%), and different ATC and ATC HPβCD melting points (172 °C e 235 °C, respectively). The mean of cell viability was 31.87% and 63.17% for 20-mM ATC and 20-mM ATC HPβCD, respectively. Moreover, remarkable cell toxicity was observed with free ATC by immunofluorescence. These results indicate the ATC HPβCD complex could be used to improve the safety of ATC. Further research are needed to establish the anesthetic safety and effectiveness in vivo.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physiological Sciences Piracicaba Dental School University of Campinas – UNICAMP, Avenida Limeira, 901Laboratory of Inflammation and Immunology Guarulhos University, Praça Teresa Cristina, 229 - CentroParana Federal Technological University – Londrina City, Avenida dos Pioneiros 3131, Jd MorumbiDepartment of Biochemistry Biology Institute University of Campinas – UNICAMP, Rua Monteiro Lobato, 255Laboratory of Environmental Nanotechnology Institute of Science and Technology of Sorocaba São Paulo State University (UNESP), Av. Três de Março, 511 - AparecidinhaLaboratory of Environmental Nanotechnology Institute of Science and Technology of Sorocaba São Paulo State University (UNESP), Av. Três de Março, 511 - AparecidinhaFAPESP: #2015/20942-6Universidade Estadual de Campinas (UNICAMP)Guarulhos UniversityParana Federal Technological University – Londrina CityUniversidade Estadual Paulista (Unesp)Burga-Sánchez, JonnyFerreira, Luiz Eduardo NunesVolpato, Maria CristinaCabeça, Luis FernandoBraga, MarioFraceto, Leonardo Fernandes [UNESP]de Paula, EneidaGroppo, Francisco Carlos2020-12-12T02:07:07Z2020-12-12T02:07:07Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1313-1323http://dx.doi.org/10.1007/s00210-020-01879-1Naunyn-Schmiedeberg's Archives of Pharmacology, v. 393, n. 7, p. 1313-1323, 2020.1432-19120028-1298http://hdl.handle.net/11449/20045610.1007/s00210-020-01879-12-s2.0-85085129449Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNaunyn-Schmiedeberg's Archives of Pharmacologyinfo:eu-repo/semantics/openAccess2021-10-23T12:40:04Zoai:repositorio.unesp.br:11449/200456Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:44:44.135358Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex
title Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex
spellingShingle Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex
Burga-Sánchez, Jonny
Articaine
Cell viability
Human gingival fibroblast
Hydroxypropyl-β-cyclodextrin
title_short Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex
title_full Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex
title_fullStr Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex
title_full_unstemmed Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex
title_sort Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex
author Burga-Sánchez, Jonny
author_facet Burga-Sánchez, Jonny
Ferreira, Luiz Eduardo Nunes
Volpato, Maria Cristina
Cabeça, Luis Fernando
Braga, Mario
Fraceto, Leonardo Fernandes [UNESP]
de Paula, Eneida
Groppo, Francisco Carlos
author_role author
author2 Ferreira, Luiz Eduardo Nunes
Volpato, Maria Cristina
Cabeça, Luis Fernando
Braga, Mario
Fraceto, Leonardo Fernandes [UNESP]
de Paula, Eneida
Groppo, Francisco Carlos
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Guarulhos University
Parana Federal Technological University – Londrina City
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Burga-Sánchez, Jonny
Ferreira, Luiz Eduardo Nunes
Volpato, Maria Cristina
Cabeça, Luis Fernando
Braga, Mario
Fraceto, Leonardo Fernandes [UNESP]
de Paula, Eneida
Groppo, Francisco Carlos
dc.subject.por.fl_str_mv Articaine
Cell viability
Human gingival fibroblast
Hydroxypropyl-β-cyclodextrin
topic Articaine
Cell viability
Human gingival fibroblast
Hydroxypropyl-β-cyclodextrin
description Articaine (ATC) is one of the most widely used local anesthetics in dentistry. Despite its safety, local toxicity has been reported. This study aimed to develop an ATC-2- hydroxypropyl-β-cyclodextrin inclusion complex (ATC HPβCD) and to assess its toxicity in vitro. The inclusion complex was performed by solubilization, followed by a fluorimetric and job plot assay to determine the complex stoichiometry. Scanning electron microscopy, DOSY- 1 H-NMR, differential scanning calorimetry (DSC), and sustained release kinetics were used to confirm the inclusion complex formation. In vitro cytotoxicity was analyzed by MTT assay and immunofluorescence in HGF cells. Fluorimetric and job plot assay determined the inclusion complex stoichiometry (ATC:HPβCD = 1:1) and complex formation time (400 min), as indicated by a strong host/guest interaction (Ka = 117.8 M − 1), complexed fraction (f = 41.4%), and different ATC and ATC HPβCD melting points (172 °C e 235 °C, respectively). The mean of cell viability was 31.87% and 63.17% for 20-mM ATC and 20-mM ATC HPβCD, respectively. Moreover, remarkable cell toxicity was observed with free ATC by immunofluorescence. These results indicate the ATC HPβCD complex could be used to improve the safety of ATC. Further research are needed to establish the anesthetic safety and effectiveness in vivo.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:07:07Z
2020-12-12T02:07:07Z
2020-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00210-020-01879-1
Naunyn-Schmiedeberg's Archives of Pharmacology, v. 393, n. 7, p. 1313-1323, 2020.
1432-1912
0028-1298
http://hdl.handle.net/11449/200456
10.1007/s00210-020-01879-1
2-s2.0-85085129449
url http://dx.doi.org/10.1007/s00210-020-01879-1
http://hdl.handle.net/11449/200456
identifier_str_mv Naunyn-Schmiedeberg's Archives of Pharmacology, v. 393, n. 7, p. 1313-1323, 2020.
1432-1912
0028-1298
10.1007/s00210-020-01879-1
2-s2.0-85085129449
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Naunyn-Schmiedeberg's Archives of Pharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1313-1323
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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