Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00210-020-01879-1 http://hdl.handle.net/11449/200456 |
Resumo: | Articaine (ATC) is one of the most widely used local anesthetics in dentistry. Despite its safety, local toxicity has been reported. This study aimed to develop an ATC-2- hydroxypropyl-β-cyclodextrin inclusion complex (ATC HPβCD) and to assess its toxicity in vitro. The inclusion complex was performed by solubilization, followed by a fluorimetric and job plot assay to determine the complex stoichiometry. Scanning electron microscopy, DOSY- 1 H-NMR, differential scanning calorimetry (DSC), and sustained release kinetics were used to confirm the inclusion complex formation. In vitro cytotoxicity was analyzed by MTT assay and immunofluorescence in HGF cells. Fluorimetric and job plot assay determined the inclusion complex stoichiometry (ATC:HPβCD = 1:1) and complex formation time (400 min), as indicated by a strong host/guest interaction (Ka = 117.8 M − 1), complexed fraction (f = 41.4%), and different ATC and ATC HPβCD melting points (172 °C e 235 °C, respectively). The mean of cell viability was 31.87% and 63.17% for 20-mM ATC and 20-mM ATC HPβCD, respectively. Moreover, remarkable cell toxicity was observed with free ATC by immunofluorescence. These results indicate the ATC HPβCD complex could be used to improve the safety of ATC. Further research are needed to establish the anesthetic safety and effectiveness in vivo. |
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Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complexArticaineCell viabilityHuman gingival fibroblastHydroxypropyl-β-cyclodextrinArticaine (ATC) is one of the most widely used local anesthetics in dentistry. Despite its safety, local toxicity has been reported. This study aimed to develop an ATC-2- hydroxypropyl-β-cyclodextrin inclusion complex (ATC HPβCD) and to assess its toxicity in vitro. The inclusion complex was performed by solubilization, followed by a fluorimetric and job plot assay to determine the complex stoichiometry. Scanning electron microscopy, DOSY- 1 H-NMR, differential scanning calorimetry (DSC), and sustained release kinetics were used to confirm the inclusion complex formation. In vitro cytotoxicity was analyzed by MTT assay and immunofluorescence in HGF cells. Fluorimetric and job plot assay determined the inclusion complex stoichiometry (ATC:HPβCD = 1:1) and complex formation time (400 min), as indicated by a strong host/guest interaction (Ka = 117.8 M − 1), complexed fraction (f = 41.4%), and different ATC and ATC HPβCD melting points (172 °C e 235 °C, respectively). The mean of cell viability was 31.87% and 63.17% for 20-mM ATC and 20-mM ATC HPβCD, respectively. Moreover, remarkable cell toxicity was observed with free ATC by immunofluorescence. These results indicate the ATC HPβCD complex could be used to improve the safety of ATC. Further research are needed to establish the anesthetic safety and effectiveness in vivo.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physiological Sciences Piracicaba Dental School University of Campinas – UNICAMP, Avenida Limeira, 901Laboratory of Inflammation and Immunology Guarulhos University, Praça Teresa Cristina, 229 - CentroParana Federal Technological University – Londrina City, Avenida dos Pioneiros 3131, Jd MorumbiDepartment of Biochemistry Biology Institute University of Campinas – UNICAMP, Rua Monteiro Lobato, 255Laboratory of Environmental Nanotechnology Institute of Science and Technology of Sorocaba São Paulo State University (UNESP), Av. Três de Março, 511 - AparecidinhaLaboratory of Environmental Nanotechnology Institute of Science and Technology of Sorocaba São Paulo State University (UNESP), Av. Três de Março, 511 - AparecidinhaFAPESP: #2015/20942-6Universidade Estadual de Campinas (UNICAMP)Guarulhos UniversityParana Federal Technological University – Londrina CityUniversidade Estadual Paulista (Unesp)Burga-Sánchez, JonnyFerreira, Luiz Eduardo NunesVolpato, Maria CristinaCabeça, Luis FernandoBraga, MarioFraceto, Leonardo Fernandes [UNESP]de Paula, EneidaGroppo, Francisco Carlos2020-12-12T02:07:07Z2020-12-12T02:07:07Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1313-1323http://dx.doi.org/10.1007/s00210-020-01879-1Naunyn-Schmiedeberg's Archives of Pharmacology, v. 393, n. 7, p. 1313-1323, 2020.1432-19120028-1298http://hdl.handle.net/11449/20045610.1007/s00210-020-01879-12-s2.0-85085129449Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNaunyn-Schmiedeberg's Archives of Pharmacologyinfo:eu-repo/semantics/openAccess2021-10-23T12:40:04Zoai:repositorio.unesp.br:11449/200456Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:44:44.135358Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex |
title |
Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex |
spellingShingle |
Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex Burga-Sánchez, Jonny Articaine Cell viability Human gingival fibroblast Hydroxypropyl-β-cyclodextrin |
title_short |
Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex |
title_full |
Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex |
title_fullStr |
Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex |
title_full_unstemmed |
Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex |
title_sort |
Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex |
author |
Burga-Sánchez, Jonny |
author_facet |
Burga-Sánchez, Jonny Ferreira, Luiz Eduardo Nunes Volpato, Maria Cristina Cabeça, Luis Fernando Braga, Mario Fraceto, Leonardo Fernandes [UNESP] de Paula, Eneida Groppo, Francisco Carlos |
author_role |
author |
author2 |
Ferreira, Luiz Eduardo Nunes Volpato, Maria Cristina Cabeça, Luis Fernando Braga, Mario Fraceto, Leonardo Fernandes [UNESP] de Paula, Eneida Groppo, Francisco Carlos |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Guarulhos University Parana Federal Technological University – Londrina City Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Burga-Sánchez, Jonny Ferreira, Luiz Eduardo Nunes Volpato, Maria Cristina Cabeça, Luis Fernando Braga, Mario Fraceto, Leonardo Fernandes [UNESP] de Paula, Eneida Groppo, Francisco Carlos |
dc.subject.por.fl_str_mv |
Articaine Cell viability Human gingival fibroblast Hydroxypropyl-β-cyclodextrin |
topic |
Articaine Cell viability Human gingival fibroblast Hydroxypropyl-β-cyclodextrin |
description |
Articaine (ATC) is one of the most widely used local anesthetics in dentistry. Despite its safety, local toxicity has been reported. This study aimed to develop an ATC-2- hydroxypropyl-β-cyclodextrin inclusion complex (ATC HPβCD) and to assess its toxicity in vitro. The inclusion complex was performed by solubilization, followed by a fluorimetric and job plot assay to determine the complex stoichiometry. Scanning electron microscopy, DOSY- 1 H-NMR, differential scanning calorimetry (DSC), and sustained release kinetics were used to confirm the inclusion complex formation. In vitro cytotoxicity was analyzed by MTT assay and immunofluorescence in HGF cells. Fluorimetric and job plot assay determined the inclusion complex stoichiometry (ATC:HPβCD = 1:1) and complex formation time (400 min), as indicated by a strong host/guest interaction (Ka = 117.8 M − 1), complexed fraction (f = 41.4%), and different ATC and ATC HPβCD melting points (172 °C e 235 °C, respectively). The mean of cell viability was 31.87% and 63.17% for 20-mM ATC and 20-mM ATC HPβCD, respectively. Moreover, remarkable cell toxicity was observed with free ATC by immunofluorescence. These results indicate the ATC HPβCD complex could be used to improve the safety of ATC. Further research are needed to establish the anesthetic safety and effectiveness in vivo. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:07:07Z 2020-12-12T02:07:07Z 2020-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00210-020-01879-1 Naunyn-Schmiedeberg's Archives of Pharmacology, v. 393, n. 7, p. 1313-1323, 2020. 1432-1912 0028-1298 http://hdl.handle.net/11449/200456 10.1007/s00210-020-01879-1 2-s2.0-85085129449 |
url |
http://dx.doi.org/10.1007/s00210-020-01879-1 http://hdl.handle.net/11449/200456 |
identifier_str_mv |
Naunyn-Schmiedeberg's Archives of Pharmacology, v. 393, n. 7, p. 1313-1323, 2020. 1432-1912 0028-1298 10.1007/s00210-020-01879-1 2-s2.0-85085129449 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Naunyn-Schmiedeberg's Archives of Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1313-1323 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128972565774336 |