Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture

Detalhes bibliográficos
Autor(a) principal: Santos, Sara C.
Data de Publicação: 2024
Outros Autores: Custódio, Catarina A., Mano, João F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/42009
Resumo: Cardiovascular diseases are a major global cause of morbidity and mortality, and they are often characterized by cardiomyocytes dead that ultimately leads to myocardial ischemia (MI). This condition replaces functional cardiac tissue with fibrotic scar tissue compromising heart function. Injectable systems for the in situ delivery of cells or molecules to assist during tissue repair have emerged as promising approaches for tissue engineering, particularly for myocardial repair. Methacryloyl platelet lysates (PLMA) have been employed for constructing full human-based 3D cell culture matrices and demonstrated potential for xeno-free applications. In this study, we propose using PLMA to produce microparticles (MPs) serving as anchors for cardiac and endothelial cells and ultimately as injectable systems for cardiac tissue repair. The herein reported PLMA MPs were produced by droplet microfluidics and showed great properties for cell attachment. More importantly, it is possible to show the capacity of PLMA MPs to serve as cell microcarriers even in the absence of animal-derived serum supplementation in the culture media.
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spelling Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell cultureMicroparticlesHuman proteinsMethacryloyl platelet lysatesMicrofluidicsCardiac tissue repairCardiovascular diseases are a major global cause of morbidity and mortality, and they are often characterized by cardiomyocytes dead that ultimately leads to myocardial ischemia (MI). This condition replaces functional cardiac tissue with fibrotic scar tissue compromising heart function. Injectable systems for the in situ delivery of cells or molecules to assist during tissue repair have emerged as promising approaches for tissue engineering, particularly for myocardial repair. Methacryloyl platelet lysates (PLMA) have been employed for constructing full human-based 3D cell culture matrices and demonstrated potential for xeno-free applications. In this study, we propose using PLMA to produce microparticles (MPs) serving as anchors for cardiac and endothelial cells and ultimately as injectable systems for cardiac tissue repair. The herein reported PLMA MPs were produced by droplet microfluidics and showed great properties for cell attachment. More importantly, it is possible to show the capacity of PLMA MPs to serve as cell microcarriers even in the absence of animal-derived serum supplementation in the culture media.Royal Society of Chemistry2025-06-21T00:00:00Z2024-06-21T00:00:00Z2024-06-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/42009eng2047-484910.1039/D3BM01933KSantos, Sara C.Custódio, Catarina A.Mano, João F.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-06-17T01:45:15Zoai:ria.ua.pt:10773/42009Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-06-17T01:45:15Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture
title Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture
spellingShingle Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture
Santos, Sara C.
Microparticles
Human proteins
Methacryloyl platelet lysates
Microfluidics
Cardiac tissue repair
title_short Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture
title_full Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture
title_fullStr Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture
title_full_unstemmed Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture
title_sort Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture
author Santos, Sara C.
author_facet Santos, Sara C.
Custódio, Catarina A.
Mano, João F.
author_role author
author2 Custódio, Catarina A.
Mano, João F.
author2_role author
author
dc.contributor.author.fl_str_mv Santos, Sara C.
Custódio, Catarina A.
Mano, João F.
dc.subject.por.fl_str_mv Microparticles
Human proteins
Methacryloyl platelet lysates
Microfluidics
Cardiac tissue repair
topic Microparticles
Human proteins
Methacryloyl platelet lysates
Microfluidics
Cardiac tissue repair
description Cardiovascular diseases are a major global cause of morbidity and mortality, and they are often characterized by cardiomyocytes dead that ultimately leads to myocardial ischemia (MI). This condition replaces functional cardiac tissue with fibrotic scar tissue compromising heart function. Injectable systems for the in situ delivery of cells or molecules to assist during tissue repair have emerged as promising approaches for tissue engineering, particularly for myocardial repair. Methacryloyl platelet lysates (PLMA) have been employed for constructing full human-based 3D cell culture matrices and demonstrated potential for xeno-free applications. In this study, we propose using PLMA to produce microparticles (MPs) serving as anchors for cardiac and endothelial cells and ultimately as injectable systems for cardiac tissue repair. The herein reported PLMA MPs were produced by droplet microfluidics and showed great properties for cell attachment. More importantly, it is possible to show the capacity of PLMA MPs to serve as cell microcarriers even in the absence of animal-derived serum supplementation in the culture media.
publishDate 2024
dc.date.none.fl_str_mv 2024-06-21T00:00:00Z
2024-06-21
2025-06-21T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/42009
url http://hdl.handle.net/10773/42009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2047-4849
10.1039/D3BM01933K
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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