Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/42009 |
Resumo: | Cardiovascular diseases are a major global cause of morbidity and mortality, and they are often characterized by cardiomyocytes dead that ultimately leads to myocardial ischemia (MI). This condition replaces functional cardiac tissue with fibrotic scar tissue compromising heart function. Injectable systems for the in situ delivery of cells or molecules to assist during tissue repair have emerged as promising approaches for tissue engineering, particularly for myocardial repair. Methacryloyl platelet lysates (PLMA) have been employed for constructing full human-based 3D cell culture matrices and demonstrated potential for xeno-free applications. In this study, we propose using PLMA to produce microparticles (MPs) serving as anchors for cardiac and endothelial cells and ultimately as injectable systems for cardiac tissue repair. The herein reported PLMA MPs were produced by droplet microfluidics and showed great properties for cell attachment. More importantly, it is possible to show the capacity of PLMA MPs to serve as cell microcarriers even in the absence of animal-derived serum supplementation in the culture media. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell cultureMicroparticlesHuman proteinsMethacryloyl platelet lysatesMicrofluidicsCardiac tissue repairCardiovascular diseases are a major global cause of morbidity and mortality, and they are often characterized by cardiomyocytes dead that ultimately leads to myocardial ischemia (MI). This condition replaces functional cardiac tissue with fibrotic scar tissue compromising heart function. Injectable systems for the in situ delivery of cells or molecules to assist during tissue repair have emerged as promising approaches for tissue engineering, particularly for myocardial repair. Methacryloyl platelet lysates (PLMA) have been employed for constructing full human-based 3D cell culture matrices and demonstrated potential for xeno-free applications. In this study, we propose using PLMA to produce microparticles (MPs) serving as anchors for cardiac and endothelial cells and ultimately as injectable systems for cardiac tissue repair. The herein reported PLMA MPs were produced by droplet microfluidics and showed great properties for cell attachment. More importantly, it is possible to show the capacity of PLMA MPs to serve as cell microcarriers even in the absence of animal-derived serum supplementation in the culture media.Royal Society of Chemistry2025-06-21T00:00:00Z2024-06-21T00:00:00Z2024-06-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/42009eng2047-484910.1039/D3BM01933KSantos, Sara C.Custódio, Catarina A.Mano, João F.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-06-17T01:45:15Zoai:ria.ua.pt:10773/42009Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-06-17T01:45:15Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture |
title |
Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture |
spellingShingle |
Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture Santos, Sara C. Microparticles Human proteins Methacryloyl platelet lysates Microfluidics Cardiac tissue repair |
title_short |
Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture |
title_full |
Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture |
title_fullStr |
Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture |
title_full_unstemmed |
Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture |
title_sort |
Photocrosslinkable microgels derived from human platelet lysates: injectable biomaterials for cardiac cell culture |
author |
Santos, Sara C. |
author_facet |
Santos, Sara C. Custódio, Catarina A. Mano, João F. |
author_role |
author |
author2 |
Custódio, Catarina A. Mano, João F. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Santos, Sara C. Custódio, Catarina A. Mano, João F. |
dc.subject.por.fl_str_mv |
Microparticles Human proteins Methacryloyl platelet lysates Microfluidics Cardiac tissue repair |
topic |
Microparticles Human proteins Methacryloyl platelet lysates Microfluidics Cardiac tissue repair |
description |
Cardiovascular diseases are a major global cause of morbidity and mortality, and they are often characterized by cardiomyocytes dead that ultimately leads to myocardial ischemia (MI). This condition replaces functional cardiac tissue with fibrotic scar tissue compromising heart function. Injectable systems for the in situ delivery of cells or molecules to assist during tissue repair have emerged as promising approaches for tissue engineering, particularly for myocardial repair. Methacryloyl platelet lysates (PLMA) have been employed for constructing full human-based 3D cell culture matrices and demonstrated potential for xeno-free applications. In this study, we propose using PLMA to produce microparticles (MPs) serving as anchors for cardiac and endothelial cells and ultimately as injectable systems for cardiac tissue repair. The herein reported PLMA MPs were produced by droplet microfluidics and showed great properties for cell attachment. More importantly, it is possible to show the capacity of PLMA MPs to serve as cell microcarriers even in the absence of animal-derived serum supplementation in the culture media. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-06-21T00:00:00Z 2024-06-21 2025-06-21T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/42009 |
url |
http://hdl.handle.net/10773/42009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2047-4849 10.1039/D3BM01933K |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817546046727782400 |