Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration

Detalhes bibliográficos
Autor(a) principal: Carvalho, Joana R.
Data de Publicação: 2019
Outros Autores: Fortunato, Isabela C., Fonseca, Catarina, Pezzarossa, Anna, Barbacena, Pedro, Dominguez-Cejudo, Maria Angeles, Vasconcelos, Francisca, Santos, Nuno C., Carvalho, Filomena Almeida, Franco, Claudio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/48132
Resumo: © 2019, Carvalho et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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spelling Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration© 2019, Carvalho et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.Morphogenesis of hierarchical vascular networks depends on the integration of multiple biomechanical signals by endothelial cells, the cells lining the interior of blood vessels. Expansion of vascular networks arises through sprouting angiogenesis, a process involving extensive cell rearrangements and collective cell migration. Yet, the mechanisms controlling angiogenic collective behavior remain poorly understood. Here, we show this collective cell behavior is regulated by non-canonical Wnt signaling. We identify that Wnt5a specifically activates Cdc42 at cell junctions downstream of ROR2 to reinforce coupling between adherens junctions and the actin cytoskeleton. We show that Wnt5a signaling stabilizes vinculin binding to alpha-catenin, and abrogation of vinculin in vivo and in vitro leads to uncoordinated polarity and deficient sprouting angiogenesis in Mus musculus. Our findings highlight how non-canonical Wnt signaling coordinates collective cell behavior during vascular morphogenesis by fine-tuning junctional mechanocoupling between endothelial cells.Research was supported by European Research Council starting grant (679368), the H2020-Twinning grant (692322), the Fundação para a Ciência e a Tecnologia funding (grants: IF/00412/2012; EXPL-BEX-BCM-2258–2013; PRECISE-LISBOA-01–0145-FEDER-016394; UID/BIM/50005/2019, a project funded by Fundação para a Ciência e a Tecnologia (FCT)/ Ministério da Ciência,Tecnologia e Ensino Superior (MCTES) through Fundos do Orçamento de Estado; and a grant from the Fondation Leducq (17CVD03); and personal fellowships: BD/52224/2013​​ to JRC, BD/105856/2014 to PB, and BD/128375/2017 to CF) and LISBOA-01–0145-FEDER-007391, project cofunded by FEDER, through POR Lisboa 2020 - Programa Operacional Regional de Lisboa, PORTUGAL 2020, and Fundação para a Ciência e a Tecnologia.eLife Sciences PublicationsRepositório da Universidade de LisboaCarvalho, Joana R.Fortunato, Isabela C.Fonseca, CatarinaPezzarossa, AnnaBarbacena, PedroDominguez-Cejudo, Maria AngelesVasconcelos, FranciscaSantos, Nuno C.Carvalho, Filomena AlmeidaFranco, Claudio2021-05-25T12:08:55Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/48132engElife. 2019 Jun 27;8:e4585310.7554/eLife.458532050-084Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:51:22Zoai:repositorio.ul.pt:10451/48132Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:02.968305Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
title Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
spellingShingle Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
Carvalho, Joana R.
title_short Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
title_full Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
title_fullStr Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
title_full_unstemmed Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
title_sort Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
author Carvalho, Joana R.
author_facet Carvalho, Joana R.
Fortunato, Isabela C.
Fonseca, Catarina
Pezzarossa, Anna
Barbacena, Pedro
Dominguez-Cejudo, Maria Angeles
Vasconcelos, Francisca
Santos, Nuno C.
Carvalho, Filomena Almeida
Franco, Claudio
author_role author
author2 Fortunato, Isabela C.
Fonseca, Catarina
Pezzarossa, Anna
Barbacena, Pedro
Dominguez-Cejudo, Maria Angeles
Vasconcelos, Francisca
Santos, Nuno C.
Carvalho, Filomena Almeida
Franco, Claudio
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Joana R.
Fortunato, Isabela C.
Fonseca, Catarina
Pezzarossa, Anna
Barbacena, Pedro
Dominguez-Cejudo, Maria Angeles
Vasconcelos, Francisca
Santos, Nuno C.
Carvalho, Filomena Almeida
Franco, Claudio
description © 2019, Carvalho et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
2021-05-25T12:08:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/48132
url http://hdl.handle.net/10451/48132
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Elife. 2019 Jun 27;8:e45853
10.7554/eLife.45853
2050-084X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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