Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/48132 |
Resumo: | © 2019, Carvalho et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
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Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration© 2019, Carvalho et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.Morphogenesis of hierarchical vascular networks depends on the integration of multiple biomechanical signals by endothelial cells, the cells lining the interior of blood vessels. Expansion of vascular networks arises through sprouting angiogenesis, a process involving extensive cell rearrangements and collective cell migration. Yet, the mechanisms controlling angiogenic collective behavior remain poorly understood. Here, we show this collective cell behavior is regulated by non-canonical Wnt signaling. We identify that Wnt5a specifically activates Cdc42 at cell junctions downstream of ROR2 to reinforce coupling between adherens junctions and the actin cytoskeleton. We show that Wnt5a signaling stabilizes vinculin binding to alpha-catenin, and abrogation of vinculin in vivo and in vitro leads to uncoordinated polarity and deficient sprouting angiogenesis in Mus musculus. Our findings highlight how non-canonical Wnt signaling coordinates collective cell behavior during vascular morphogenesis by fine-tuning junctional mechanocoupling between endothelial cells.Research was supported by European Research Council starting grant (679368), the H2020-Twinning grant (692322), the Fundação para a Ciência e a Tecnologia funding (grants: IF/00412/2012; EXPL-BEX-BCM-2258–2013; PRECISE-LISBOA-01–0145-FEDER-016394; UID/BIM/50005/2019, a project funded by Fundação para a Ciência e a Tecnologia (FCT)/ Ministério da Ciência,Tecnologia e Ensino Superior (MCTES) through Fundos do Orçamento de Estado; and a grant from the Fondation Leducq (17CVD03); and personal fellowships: BD/52224/2013 to JRC, BD/105856/2014 to PB, and BD/128375/2017 to CF) and LISBOA-01–0145-FEDER-007391, project cofunded by FEDER, through POR Lisboa 2020 - Programa Operacional Regional de Lisboa, PORTUGAL 2020, and Fundação para a Ciência e a Tecnologia.eLife Sciences PublicationsRepositório da Universidade de LisboaCarvalho, Joana R.Fortunato, Isabela C.Fonseca, CatarinaPezzarossa, AnnaBarbacena, PedroDominguez-Cejudo, Maria AngelesVasconcelos, FranciscaSantos, Nuno C.Carvalho, Filomena AlmeidaFranco, Claudio2021-05-25T12:08:55Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/48132engElife. 2019 Jun 27;8:e4585310.7554/eLife.458532050-084Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:51:22Zoai:repositorio.ul.pt:10451/48132Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:02.968305Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration |
title |
Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration |
spellingShingle |
Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration Carvalho, Joana R. |
title_short |
Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration |
title_full |
Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration |
title_fullStr |
Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration |
title_full_unstemmed |
Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration |
title_sort |
Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration |
author |
Carvalho, Joana R. |
author_facet |
Carvalho, Joana R. Fortunato, Isabela C. Fonseca, Catarina Pezzarossa, Anna Barbacena, Pedro Dominguez-Cejudo, Maria Angeles Vasconcelos, Francisca Santos, Nuno C. Carvalho, Filomena Almeida Franco, Claudio |
author_role |
author |
author2 |
Fortunato, Isabela C. Fonseca, Catarina Pezzarossa, Anna Barbacena, Pedro Dominguez-Cejudo, Maria Angeles Vasconcelos, Francisca Santos, Nuno C. Carvalho, Filomena Almeida Franco, Claudio |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Carvalho, Joana R. Fortunato, Isabela C. Fonseca, Catarina Pezzarossa, Anna Barbacena, Pedro Dominguez-Cejudo, Maria Angeles Vasconcelos, Francisca Santos, Nuno C. Carvalho, Filomena Almeida Franco, Claudio |
description |
© 2019, Carvalho et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z 2021-05-25T12:08:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/48132 |
url |
http://hdl.handle.net/10451/48132 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Elife. 2019 Jun 27;8:e45853 10.7554/eLife.45853 2050-084X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
eLife Sciences Publications |
publisher.none.fl_str_mv |
eLife Sciences Publications |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134546759778304 |