Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/8051 |
Resumo: | (This article belongs to the Collection New Insights into Bacterial Pathogenesis) |
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Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleriAliarcobacter butzleriNatural CompetenceTransformationInfecções Gastrointestinais(This article belongs to the Collection New Insights into Bacterial Pathogenesis)Aliarcobacter butzleri is an emergent enteropathogen, showing high genetic diversity, which likely contributes to its adaptive capacity to different environments. Whether natural transformation can be a mechanism that generates genetic diversity in A. butzleri is still unknown. In the present study, we aimed to establish if A. butzleri is naturally competent for transformation and to investigate the factors influencing this process. Two different transformation procedures were tested using exogenous and isogenic DNA containing antibiotic resistance markers, and different external conditions influencing the process were evaluated. The highest number of transformable A. butzleri strains were obtained with the agar transformation method when compared to the biphasic system (65% versus 47%). A. butzleri was able to uptake isogenic chromosomal DNA at different growth phases, and the competence state was maintained from the exponential to the stationary phases. Overall, the optimal conditions for transformation with the biphasic system were the use of 1 μg of isogenic DNA and incubation at 30 °C under a microaerobic atmosphere, resulting in a transformation frequency ~8 × 10-6 transformants/CFU. We also observed that A. butzleri favored the transformation with the genetic material of its own strain/species, with the DNA incorporation process occurring promptly after the addition of genomic material. In addition, we observed that A. butzleri strains could exchange genetic material in co-culture assays. The presence of homologs of well-known genes involved in the competence in the A. butzleri genome corroborates the natural competence of this species. In conclusion, our results show that A. butzleri is a naturally transformable species, suggesting that horizontal gene transfer mediated by natural transformation is one of the processes contributing to its genetic diversity. In addition, natural transformation can be used as a tool for genetic studies of this species.This work was supported by the Foundation for Science and Technology (FCT), through funds from the State Budget, and by the European Regional Development Fund (ERDF), under the Portugal 2020 Program, through the Regional Operational Program of the Center (Centro2020), through the Project with the reference UIDB/00709/2020. This work was also funded by the operation CENTRO-01-0145-FEDER-000019 – C4 – Centro de Competências em Cloud Computing, supported by the European Regional Development Fund (ERDF) through the Regional Operational Program of the Center (Centro2020). Cristiana Mateus is the recipient of a doctoral fellowship (UI/BD/151023/2021) under the scope of the CICS-UBI Programmatic Funding (UIDP/00709/2020). Susana Ferreira acknowledges the Universidade da Beira Interior and FCT by the contract of Scientific Employment according to DL57/2016.MDPIRepositório Científico do Instituto Nacional de SaúdeBonifácio, MarinaMateus, CristianaAlves, Ana R.Maldonado, EmanuelDuarte, Ana P.Domingues, FernandaOleastro, MónicaFerreira, Susana2022-07-03T16:03:16Z2021-07-192021-07-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8051engPathogens. 2021 Jul 19;10(7):909. doi: 10.3390/pathogens100709092076-081710.3390/pathogens10070909info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:25Zoai:repositorio.insa.pt:10400.18/8051Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:49.941491Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri |
| title |
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri |
| spellingShingle |
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri Bonifácio, Marina Aliarcobacter butzleri Natural Competence Transformation Infecções Gastrointestinais |
| title_short |
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri |
| title_full |
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri |
| title_fullStr |
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri |
| title_full_unstemmed |
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri |
| title_sort |
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri |
| author |
Bonifácio, Marina |
| author_facet |
Bonifácio, Marina Mateus, Cristiana Alves, Ana R. Maldonado, Emanuel Duarte, Ana P. Domingues, Fernanda Oleastro, Mónica Ferreira, Susana |
| author_role |
author |
| author2 |
Mateus, Cristiana Alves, Ana R. Maldonado, Emanuel Duarte, Ana P. Domingues, Fernanda Oleastro, Mónica Ferreira, Susana |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Bonifácio, Marina Mateus, Cristiana Alves, Ana R. Maldonado, Emanuel Duarte, Ana P. Domingues, Fernanda Oleastro, Mónica Ferreira, Susana |
| dc.subject.por.fl_str_mv |
Aliarcobacter butzleri Natural Competence Transformation Infecções Gastrointestinais |
| topic |
Aliarcobacter butzleri Natural Competence Transformation Infecções Gastrointestinais |
| description |
(This article belongs to the Collection New Insights into Bacterial Pathogenesis) |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-07-19 2021-07-19T00:00:00Z 2022-07-03T16:03:16Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10400.18/8051 |
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http://hdl.handle.net/10400.18/8051 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Pathogens. 2021 Jul 19;10(7):909. doi: 10.3390/pathogens10070909 2076-0817 10.3390/pathogens10070909 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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