RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri

Detalhes bibliográficos
Autor(a) principal: Mateus, Cristiana
Data de Publicação: 2021
Outros Autores: Nunes, Ana Rita, Oleastro, Mónica, Domingues, Fernanda, Ferreira, Susana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8048
Resumo: (This article belongs to the Special Issue The Structural and Functional Study of Efflux Pumps Belonging to the RND Transporters Family from Gram-Negative Bacteria)
id RCAP_849468fc4ed75546b45e7ea535ff9ec5
oai_identifier_str oai:repositorio.insa.pt:10400.18/8048
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleriAliarcobacter butzleriRND Efflux PumpsResistanceVirulenceInfecções Gastrointestinais(This article belongs to the Special Issue The Structural and Functional Study of Efflux Pumps Belonging to the RND Transporters Family from Gram-Negative Bacteria)Aliarcobacter butzleri is an emergent enteropathogen that can be found in a range of environments. This bacterium presents a vast repertoire of efflux pumps, such as the ones belonging to the resistance nodulation cell division family, which may be associated with bacterial resistance, as well as virulence. Thus, this work aimed to evaluate the contribution of three RND efflux systems, AreABC, AreDEF and AreGHI, in the resistance and virulence of A. butzleri. Mutant strains were constructed by inactivation of the gene that encodes the inner membrane protein of these systems. The bacterial resistance profile of parental and mutant strains to several antimicrobials was assessed, as was the intracellular accumulation of the ethidium bromide dye. Regarding bacterial virulence, the role of these three efflux pumps on growth, strain fitness, motility, biofilm formation ability, survival in adverse conditions (oxidative stress and bile salts) and human serum and in vitro adhesion and invasion to Caco-2 cells was evaluated. We observed that the mutants from the three efflux pumps were more susceptible to several classes of antimicrobials than the parental strain and presented an increase in the accumulation of ethidium bromide, indicating a potential role of the efflux pumps in the extrusion of antimicrobials. The mutant strains had no bacterial growth defects; nonetheless, they presented a reduction in relative fitness. For the three mutants, an increase in the susceptibility to oxidative stress was observed, while only the mutant for AreGHI efflux pump showed a relevant role in bile stress survival. All the mutant strains showed an impairment in biofilm formation ability, were more susceptible to human serum and were less adherent to intestinal epithelial cells. Overall, the results support the contribution of the efflux pumps AreABC, AreDEF and AreGHI of A. butzleri to antimicrobial resistance, as well as to bacterial virulence.This work was financed by the Foundation for Science and Technology (FCT), through funds from the State Budget, and by the European Regional Development Fund (ERDF), under the Portugal 2020 Program, through the Regional Operational Program of the Center (Centro2020), through the Project with the reference UIDB/00709/2020. Cristiana Mateus is recipient of a doctoral fellowship (UI/BD/151023/2021) under the scope of the CICS-UBI Programmatic Funding (UIDP/00709/2020). Susana Ferreira acknowledges UBI and FCT by the contract of Scientific Employment according to DL57/2016.MDPIRepositório Científico do Instituto Nacional de SaúdeMateus, CristianaNunes, Ana RitaOleastro, MónicaDomingues, FernandaFerreira, Susana2022-07-03T15:45:15Z2021-07-062021-07-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8048engAntibiotics (Basel). 2021 Jul 6;10(7):823. doi: 10.3390/antibiotics100708232079-638210.3390/antibiotics10070823info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:25Zoai:repositorio.insa.pt:10400.18/8048Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:49.777321Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri
title RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri
spellingShingle RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri
Mateus, Cristiana
Aliarcobacter butzleri
RND Efflux Pumps
Resistance
Virulence
Infecções Gastrointestinais
title_short RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri
title_full RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri
title_fullStr RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri
title_full_unstemmed RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri
title_sort RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri
author Mateus, Cristiana
author_facet Mateus, Cristiana
Nunes, Ana Rita
Oleastro, Mónica
Domingues, Fernanda
Ferreira, Susana
author_role author
author2 Nunes, Ana Rita
Oleastro, Mónica
Domingues, Fernanda
Ferreira, Susana
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Mateus, Cristiana
Nunes, Ana Rita
Oleastro, Mónica
Domingues, Fernanda
Ferreira, Susana
dc.subject.por.fl_str_mv Aliarcobacter butzleri
RND Efflux Pumps
Resistance
Virulence
Infecções Gastrointestinais
topic Aliarcobacter butzleri
RND Efflux Pumps
Resistance
Virulence
Infecções Gastrointestinais
description (This article belongs to the Special Issue The Structural and Functional Study of Efflux Pumps Belonging to the RND Transporters Family from Gram-Negative Bacteria)
publishDate 2021
dc.date.none.fl_str_mv 2021-07-06
2021-07-06T00:00:00Z
2022-07-03T15:45:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8048
url http://hdl.handle.net/10400.18/8048
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antibiotics (Basel). 2021 Jul 6;10(7):823. doi: 10.3390/antibiotics10070823
2079-6382
10.3390/antibiotics10070823
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132174672199680

Registros relacionados