Effect of crosslinking in chitosan/aloe vera-based membranes for biomedical applications

• Autor(a) principal: Silva, Simone Santos
• Data de Publicação: 2013
• Outros Autores: Caridade, S. G., Mano, J. F., Reis, R. L.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/25102
• topic: Aloe vera; Genipin; Membranes; Crosslinking; Genipin; Blends; Chitosan; Science & Technology

The  positive  interaction  between  polysaccharides  with  active  phytochemicals found  in  medicinal  plants  may  represent  a  strategy  to  create  active  wound dressing  materials  useful  for  skin  repair.  In  the  present  work,  blended membranes  composed  of  chitosan  (Cht)  and  Aloe  vera  gel  were  prepared through the solvent casting, and were crosslinked with genipin to improve their properties. Topography, swelling, wettability, mechanical properties and in vitro cellular response of the membranes were investigated. With the incorporation of aloe  vera  gel  into  chitosan  solution,  the  developed  chitosan/aloe-based membranes  displayed  increased  roughness  and  wettability;  while  the  genipin crosslinking  promoted  the  formation  of  stiffer  membranes  in  comparison  to those  of  the  non-modified  membranes.  Moreover, in  vitro cell  culture  studies evidenced that the L929 cells have high cell viability, confirmed by MTS test and calcein-AM staining. The findings suggested that both blend compositions and crosslinking  affected  the  physico-chemical  properties  and  cellular  behavior  of the developed membranes.