Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogels
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/18711 |
Resumo: | A variety of hydrogels, which correspond to a class of highly hydrated polymeric materials, are being used as biomaterials. In particular, chitosan hydrogels stand out for being an adhesive matrix that presents antibacterial, osteogenic and non-toxic properties. And they are able to retain, release and deliver therapeutic agents at the application site. The production of chitosan-based hydrogels has been achieved by mixing chitosan solutions with crosslinking agents or charged polymers under specific reaction conditions. The most commonly used reagent is glutaraldehyde, however, it has high cytotoxicity, and therefore, it has been replaced by less toxic and biologically safe crosslinkers, such as genipin, a natural reagent. Therefore, the objective of this work was to promote the extraction and purification of genipin in solid form from green fruits of Jenipapo, carrying out its identification through thin layer chromatography (TLC), mass spectrometry, infrared spectroscopy with transform Fourier test (FTIR) and quantification by gravimetric analysis. And the production of chitosan hydrogels using the obtained genipin as a crosslinking agent. The samples obtained were characterized by water absorption, Differential Scanning Calorimetry (DSC), FTIR and Rheology. The extracted genipin showed 0.47% extractive yield, while the hydrogels produced with 3% genipin showed a characteristic profile of a polymeric gel. |
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Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogelsObtención, caracterización y uso de genipina como reticulante de hidrogeles de quitosanoObtenção, caracterização e uso de genipina como agente reticulante de hidrogeis de quitosanaHidrogéisGenipinaQuitosanaAgente reticulante.HidrogelesGenipinoQuitosanoAgente de reticulación.HydrogelsGenipineChitosanCrosslinking agent.A variety of hydrogels, which correspond to a class of highly hydrated polymeric materials, are being used as biomaterials. In particular, chitosan hydrogels stand out for being an adhesive matrix that presents antibacterial, osteogenic and non-toxic properties. And they are able to retain, release and deliver therapeutic agents at the application site. The production of chitosan-based hydrogels has been achieved by mixing chitosan solutions with crosslinking agents or charged polymers under specific reaction conditions. The most commonly used reagent is glutaraldehyde, however, it has high cytotoxicity, and therefore, it has been replaced by less toxic and biologically safe crosslinkers, such as genipin, a natural reagent. Therefore, the objective of this work was to promote the extraction and purification of genipin in solid form from green fruits of Jenipapo, carrying out its identification through thin layer chromatography (TLC), mass spectrometry, infrared spectroscopy with transform Fourier test (FTIR) and quantification by gravimetric analysis. And the production of chitosan hydrogels using the obtained genipin as a crosslinking agent. The samples obtained were characterized by water absorption, Differential Scanning Calorimetry (DSC), FTIR and Rheology. The extracted genipin showed 0.47% extractive yield, while the hydrogels produced with 3% genipin showed a characteristic profile of a polymeric gel.Se están utilizando como biomateriales una variedad de hidrogeles, que corresponden a una clase de materiales poliméricos altamente hidratados. En particular, los hidrogeles de quitosano destacan por ser una matriz adhesiva que presenta propiedades antibacterianas, osteogénicas y no tóxicas. Y son capaces de retener, liberar y administrar agentes terapéuticos en el sitio de aplicación. La producción de hidrogeles a base de quitosano se ha logrado mezclando soluciones de quitosano con agentes reticulantes o polímeros cargados en condiciones de reacción específicas. El reactivo más comúnmente utilizado es el glutaraldehído, sin embargo, tiene una alta citotoxicidad y, por lo tanto, ha sido reemplazado por reticulantes menos tóxicos y biológicamente seguros, como el genipin, un reactivo natural. Por tanto, el objetivo de este trabajo fue promover la extracción y purificación de genipina en forma sólida a partir de frutos verdes de Jenipapo, realizando su identificación mediante cromatografía en capa fina (TLC), espectrometría de masas, espectroscopia infrarroja con transformada prueba de Fourier (FTIR) y cuantificación mediante análisis gravimétrico. Y la producción de hidrogeles de quitosano utilizando la genipina obtenida como reticulante. Las muestras obtenidas se caracterizaron por absorción de agua, Calorimetría Diferencial de Barrido (DSC), FTIR y Reología. La genipina extraída mostró un rendimiento extractivo del 0,47%, mientras que los hidrogeles producidos con genipina al 3% mostraron un perfil característico de un gel polimérico.Uma variedade de hidrogéis, que correspondem a uma classe de materiais poliméricos altamente hidratados, estão sendo empregados como biomateriais. De modo particular, hidrogéis de quitosana, destaca-se por ser uma matriz adesiva que apresenta, propriedades antibacterianas, osteogênicas e não toxidade. E são capazes de reter, liberar e distribuir agentes terapêuticos no local de aplicação. A produção de hidrogéis à base de quitosana tem sido alcançada por meio da mistura de soluções de quitosana com agentes reticulantes ou polímeros carregados sob condições de reações específicas. O reagente mais comumente utilizado é o glutaraldeído, no entanto, apresenta elevada citotoxicidade, e por isso, vem sendo substituídos por reticulantes menos tóxicos, e biologicamente seguros, a exemplo da genipina, um reagente natural. Portanto, o objetivo deste trabalho foi promover a extração e purificação da genipina na forma sólida a partir de frutos verdes de Jenipapo, realizando sua identificação por meio de cromatografia de camada delgada (CCD), espectrometria de massa, espectroscopia na região do infravermelho com transformada de Fourier (FTIR) e quantificação por análise gravimétrica. E a produção de hidrogéis de quitosana usando a genipina obtida, como agente reticulante. As amostras obtidas, foram caracterizadas por absorção de água, Calorimetria Exploratória Diferencial (DSC), FTIR e Reologia. A genipina extraída apresentou 0,47% de rendimento extrativo, enquanto os hidrogéis produzidos com 3% de genipina apresentaram um perfil característico de gel polimérico. Research, Society and Development2021-08-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1871110.33448/rsd-v10i10.18711Research, Society and Development; Vol. 10 No. 10; e183101018711Research, Society and Development; Vol. 10 Núm. 10; e183101018711Research, Society and Development; v. 10 n. 10; e1831010187112525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/18711/16721Copyright (c) 2021 Rafael Braga da Cunha; João Emidio da Silva Neto; Bruna de Freitas Leite; José Filipe Bacalhau Rodrigues; Maria Roberta de Oliveira Pinto; Marcus Vinicius Lia Fookhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCunha, Rafael Braga daSilva Neto, João Emidio da Leite, Bruna de FreitasRodrigues, José Filipe BacalhauPinto, Maria Roberta de Oliveira Fook, Marcus Vinicius Lia2021-10-02T21:49:16Zoai:ojs.pkp.sfu.ca:article/18711Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:46.741949Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogels Obtención, caracterización y uso de genipina como reticulante de hidrogeles de quitosano Obtenção, caracterização e uso de genipina como agente reticulante de hidrogeis de quitosana |
| title |
Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogels |
| spellingShingle |
Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogels Cunha, Rafael Braga da Hidrogéis Genipina Quitosana Agente reticulante. Hidrogeles Genipino Quitosano Agente de reticulación. Hydrogels Genipine Chitosan Crosslinking agent. |
| title_short |
Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogels |
| title_full |
Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogels |
| title_fullStr |
Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogels |
| title_full_unstemmed |
Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogels |
| title_sort |
Obtaining, characterizing and using genipin as a crosslinking agent for chitosan hydrogels |
| author |
Cunha, Rafael Braga da |
| author_facet |
Cunha, Rafael Braga da Silva Neto, João Emidio da Leite, Bruna de Freitas Rodrigues, José Filipe Bacalhau Pinto, Maria Roberta de Oliveira Fook, Marcus Vinicius Lia |
| author_role |
author |
| author2 |
Silva Neto, João Emidio da Leite, Bruna de Freitas Rodrigues, José Filipe Bacalhau Pinto, Maria Roberta de Oliveira Fook, Marcus Vinicius Lia |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Cunha, Rafael Braga da Silva Neto, João Emidio da Leite, Bruna de Freitas Rodrigues, José Filipe Bacalhau Pinto, Maria Roberta de Oliveira Fook, Marcus Vinicius Lia |
| dc.subject.por.fl_str_mv |
Hidrogéis Genipina Quitosana Agente reticulante. Hidrogeles Genipino Quitosano Agente de reticulación. Hydrogels Genipine Chitosan Crosslinking agent. |
| topic |
Hidrogéis Genipina Quitosana Agente reticulante. Hidrogeles Genipino Quitosano Agente de reticulación. Hydrogels Genipine Chitosan Crosslinking agent. |
| description |
A variety of hydrogels, which correspond to a class of highly hydrated polymeric materials, are being used as biomaterials. In particular, chitosan hydrogels stand out for being an adhesive matrix that presents antibacterial, osteogenic and non-toxic properties. And they are able to retain, release and deliver therapeutic agents at the application site. The production of chitosan-based hydrogels has been achieved by mixing chitosan solutions with crosslinking agents or charged polymers under specific reaction conditions. The most commonly used reagent is glutaraldehyde, however, it has high cytotoxicity, and therefore, it has been replaced by less toxic and biologically safe crosslinkers, such as genipin, a natural reagent. Therefore, the objective of this work was to promote the extraction and purification of genipin in solid form from green fruits of Jenipapo, carrying out its identification through thin layer chromatography (TLC), mass spectrometry, infrared spectroscopy with transform Fourier test (FTIR) and quantification by gravimetric analysis. And the production of chitosan hydrogels using the obtained genipin as a crosslinking agent. The samples obtained were characterized by water absorption, Differential Scanning Calorimetry (DSC), FTIR and Rheology. The extracted genipin showed 0.47% extractive yield, while the hydrogels produced with 3% genipin showed a characteristic profile of a polymeric gel. |
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2021 |
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2021-08-08 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/18711 10.33448/rsd-v10i10.18711 |
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https://rsdjournal.org/index.php/rsd/article/view/18711 |
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10.33448/rsd-v10i10.18711 |
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por |
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por |
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https://rsdjournal.org/index.php/rsd/article/view/18711/16721 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 10 No. 10; e183101018711 Research, Society and Development; Vol. 10 Núm. 10; e183101018711 Research, Society and Development; v. 10 n. 10; e183101018711 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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