Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents

Detalhes bibliográficos
Autor(a) principal: Batista, Miguel P.
Data de Publicação: 2022
Outros Autores: Fernández, Naiara, Gaspar, Frédéric B., Bronze, Maria do Rosário, Duarte, Ana Rita C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/143554
Resumo: ERC-2016-CoG 725034
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spelling Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solventsblue sharkskin collagenextract characterizationextraction process intensificationmarine waste valorizationnatural deep eutectic solvent (NADES)Chemistry(all)SDG 14 - Life Below WaterERC-2016-CoG 725034The disposal of large amounts of skin waste resulting from the blue shark fishing industry presents several industrial and environmental waste management concerns. In addition, these marine subproducts are interesting sources of collagen, a fibrous protein that shows high social and economic interest in a broad range of biomedical, pharmaceutical, and cosmetic applications. However, blue shark wasted skins are a poorly explored matrix for this purpose, and conventional collagen recovery methodologies involve several pre-treatment steps, long extraction times and low temperatures. This work presents a new green and sustainable collagen extraction approach using a natural deep eutectic solvent composed of citric acid:xylitol:water at a 1:1:10 molar ratio, and the chemical characterization of the extracted collagen by discontinuous electrophoresis, thermogravimetric analysis, Fourier transformed infrared spectroscopy and circular dichroism. The extracted material was a pure type I collagen, and the novel approach presented an extraction yield 2.5 times higher than the conventional one, without pre-treatment of raw material and reducing the procedure time from 96 to 1 h. Furthermore, the in vitro cytotoxicity evaluation, performed with a mouse fibroblasts cell line, has proven the biocompatibility of the extracted material. Overall, the obtained results demonstrate a simple, quick, cheap and environmentally sustainable process to obtain marine collagen with promising properties for biomedical and cosmetic applications.DQ - Departamento de QuímicaLAQV@REQUIMTEInstituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNBatista, Miguel P.Fernández, NaiaraGaspar, Frédéric B.Bronze, Maria do RosárioDuarte, Ana Rita C.2022-09-06T22:44:41Z2022-06-162022-06-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/143554eng2296-2646PURE: 46300446https://doi.org/10.3389/fchem.2022.937036info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:22:03Zoai:run.unl.pt:10362/143554Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:00.753634Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents
title Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents
spellingShingle Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents
Batista, Miguel P.
blue sharkskin collagen
extract characterization
extraction process intensification
marine waste valorization
natural deep eutectic solvent (NADES)
Chemistry(all)
SDG 14 - Life Below Water
title_short Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents
title_full Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents
title_fullStr Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents
title_full_unstemmed Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents
title_sort Extraction of Biocompatible Collagen From Blue Shark Skins Through the Conventional Extraction Process Intensification Using Natural Deep Eutectic Solvents
author Batista, Miguel P.
author_facet Batista, Miguel P.
Fernández, Naiara
Gaspar, Frédéric B.
Bronze, Maria do Rosário
Duarte, Ana Rita C.
author_role author
author2 Fernández, Naiara
Gaspar, Frédéric B.
Bronze, Maria do Rosário
Duarte, Ana Rita C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
LAQV@REQUIMTE
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Batista, Miguel P.
Fernández, Naiara
Gaspar, Frédéric B.
Bronze, Maria do Rosário
Duarte, Ana Rita C.
dc.subject.por.fl_str_mv blue sharkskin collagen
extract characterization
extraction process intensification
marine waste valorization
natural deep eutectic solvent (NADES)
Chemistry(all)
SDG 14 - Life Below Water
topic blue sharkskin collagen
extract characterization
extraction process intensification
marine waste valorization
natural deep eutectic solvent (NADES)
Chemistry(all)
SDG 14 - Life Below Water
description ERC-2016-CoG 725034
publishDate 2022
dc.date.none.fl_str_mv 2022-09-06T22:44:41Z
2022-06-16
2022-06-16T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143554
url http://hdl.handle.net/10362/143554
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2296-2646
PURE: 46300446
https://doi.org/10.3389/fchem.2022.937036
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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