Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/43244 |
Resumo: | The fishing industry produces substantial by-products, such as heads, skins, bones, and scales, rich in collagen—a prevalent protein in these materials. However, further application of deep eutectic solvent-based extraction remains unexplored. In this study, we extracted collagen with urea: propanoic acid mixture (U:PA; 1:2) with a 2.2% yield, followed by enzymatic hydrolysis with alcalase for 120 min. The resulting bioactive peptides demonstrated notable antioxidant activity (961 µmol TE) and antihypertensive properties (39.3% ACE inhibition). Subsequently, we encapsulated 39.3% of these hydrolysates in chitosan-TPP capsules, which released about 58% of their content, primarily in the intestine, as mimicked in the in vitro model of the gastrointestinal tract. Although the digestion process did not significantly alter the size of the non-encapsulated collagen peptides, it did influence their health benefits. The promising results suggest that further research could optimize the use of collagen from fish by-products, potentially offering a sustainable source for health products. |
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Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysisCodfish skinDeep eutectic solventsMarine collagenBioactive peptidesColon deliveryFishery byproductsNutraceutical releaseAtlantic codfishAtlantic codfishThe fishing industry produces substantial by-products, such as heads, skins, bones, and scales, rich in collagen—a prevalent protein in these materials. However, further application of deep eutectic solvent-based extraction remains unexplored. In this study, we extracted collagen with urea: propanoic acid mixture (U:PA; 1:2) with a 2.2% yield, followed by enzymatic hydrolysis with alcalase for 120 min. The resulting bioactive peptides demonstrated notable antioxidant activity (961 µmol TE) and antihypertensive properties (39.3% ACE inhibition). Subsequently, we encapsulated 39.3% of these hydrolysates in chitosan-TPP capsules, which released about 58% of their content, primarily in the intestine, as mimicked in the in vitro model of the gastrointestinal tract. Although the digestion process did not significantly alter the size of the non-encapsulated collagen peptides, it did influence their health benefits. The promising results suggest that further research could optimize the use of collagen from fish by-products, potentially offering a sustainable source for health products.Veritati - Repositório Institucional da Universidade Católica PortuguesaSilva, IsaVaz, Bárbara M. C.Sousa, SérgioPintado, Maria ManuelaCoscueta, Ezequiel R.Ventura, Sónia P. M.2023-11-30T12:05:17Z2024-01-012024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/43244eng0963-996910.1016/j.foodres.2023.11372985178567050001129931000001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-16T01:45:13Zoai:repositorio.ucp.pt:10400.14/43244Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:41:14.958951Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysis |
title |
Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysis |
spellingShingle |
Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysis Silva, Isa Codfish skin Deep eutectic solvents Marine collagen Bioactive peptides Colon delivery Fishery byproducts Nutraceutical release Atlantic codfish Atlantic codfish |
title_short |
Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysis |
title_full |
Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysis |
title_fullStr |
Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysis |
title_full_unstemmed |
Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysis |
title_sort |
Gastrointestinal delivery of codfish skin-derived collagen hydrolysates: deep eutectic solvent extraction and bioactivity analysis |
author |
Silva, Isa |
author_facet |
Silva, Isa Vaz, Bárbara M. C. Sousa, Sérgio Pintado, Maria Manuela Coscueta, Ezequiel R. Ventura, Sónia P. M. |
author_role |
author |
author2 |
Vaz, Bárbara M. C. Sousa, Sérgio Pintado, Maria Manuela Coscueta, Ezequiel R. Ventura, Sónia P. M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Silva, Isa Vaz, Bárbara M. C. Sousa, Sérgio Pintado, Maria Manuela Coscueta, Ezequiel R. Ventura, Sónia P. M. |
dc.subject.por.fl_str_mv |
Codfish skin Deep eutectic solvents Marine collagen Bioactive peptides Colon delivery Fishery byproducts Nutraceutical release Atlantic codfish Atlantic codfish |
topic |
Codfish skin Deep eutectic solvents Marine collagen Bioactive peptides Colon delivery Fishery byproducts Nutraceutical release Atlantic codfish Atlantic codfish |
description |
The fishing industry produces substantial by-products, such as heads, skins, bones, and scales, rich in collagen—a prevalent protein in these materials. However, further application of deep eutectic solvent-based extraction remains unexplored. In this study, we extracted collagen with urea: propanoic acid mixture (U:PA; 1:2) with a 2.2% yield, followed by enzymatic hydrolysis with alcalase for 120 min. The resulting bioactive peptides demonstrated notable antioxidant activity (961 µmol TE) and antihypertensive properties (39.3% ACE inhibition). Subsequently, we encapsulated 39.3% of these hydrolysates in chitosan-TPP capsules, which released about 58% of their content, primarily in the intestine, as mimicked in the in vitro model of the gastrointestinal tract. Although the digestion process did not significantly alter the size of the non-encapsulated collagen peptides, it did influence their health benefits. The promising results suggest that further research could optimize the use of collagen from fish by-products, potentially offering a sustainable source for health products. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-30T12:05:17Z 2024-01-01 2024-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/43244 |
url |
http://hdl.handle.net/10400.14/43244 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0963-9969 10.1016/j.foodres.2023.113729 85178567050 001129931000001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136315970682880 |