Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry

Detalhes bibliográficos
Autor(a) principal: Cerca, Nuno
Data de Publicação: 2005
Outros Autores: Martins, Silvia, Cerca, Filipe, Jefferson, Kimberly K., Pier, Gerald B., Oliveira, Rosário, Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/3648
Resumo: Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy
id RCAP_39754096af429fc96f3d069d51cb447e
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/3648
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetryNosocomial infectionsPathogensBiofilmsAntibiotic resistanceCoNSScience & TechnologyPublished by Oxford University Press on behalf of the British Society for Antimicrobial ChemotherapyObjectives: To quantitatively compare the antibiotic susceptibility of biofilms formed by the coagulasenegative staphylococci (CoNS) Staphylococcus epidermidis and Staphylococcus haemolyticus with the susceptibility of planktonic cultures. Methods: SeveralCoNSstrains were grown planktonically or as biofilms to determine the effect of themode of growth on the level of susceptibility to antibiotics with different mechanisms of action. The utility of a new, rapid colorimetric method that is based on the reduction of a tetrazolium salt (XTT) to measure cell viability was tested by comparison with standard bacterial enumeration techniques. A 6 h kinetic study was performed using dicloxacillin, cefazolin, vancomycin, tetracycline and rifampicin at the peak serum concentration of each antibiotic. Results: In planktonic cells, inhibitors of cell wall synthesis were highly effective over a 3 h period. Biofilms were much less susceptible than planktonic cultures to all antibiotics tested, particularly inhibitors of cell wall synthesis. The susceptibility to inhibitors of protein and RNA synthesis was affected by the biofilm phenotype to a lesser degree. Standard bacterial enumeration techniques and the XTT method produced equivalent results both in biofilms and planktonic assays. Conclusions: This study provides a more accurate comparison between the antibiotic susceptibilities of planktonic versus biofilm populations, because the cell densities in the two populations were similar and because we measured the concentration required to inhibit bacterial metabolism rather than to eradicate the entire bacterial population. While the biofilm phenotype is highly resistant to antibiotics that target cell wall synthesis, it is fairly susceptible to antibiotics that target RNA and protein synthesis.Fundação para a Ciência e a Tecnologia (FCT)Oxford University PressUniversidade do MinhoCerca, NunoMartins, SilviaCerca, FilipeJefferson, Kimberly K.Pier, Gerald B.Oliveira, RosárioAzeredo, Joana20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/3648eng"Journal of antimicrobial chemotherapy". ISSN 0305-7453. 56:2 (2005) 331-336.0305-745310.1093/jac/dki21715980094info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:04:11Zoai:repositorium.sdum.uminho.pt:1822/3648Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:54:25.760569Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry
title Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry
spellingShingle Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry
Cerca, Nuno
Nosocomial infections
Pathogens
Biofilms
Antibiotic resistance
CoNS
Science & Technology
title_short Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry
title_full Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry
title_fullStr Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry
title_full_unstemmed Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry
title_sort Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry
author Cerca, Nuno
author_facet Cerca, Nuno
Martins, Silvia
Cerca, Filipe
Jefferson, Kimberly K.
Pier, Gerald B.
Oliveira, Rosário
Azeredo, Joana
author_role author
author2 Martins, Silvia
Cerca, Filipe
Jefferson, Kimberly K.
Pier, Gerald B.
Oliveira, Rosário
Azeredo, Joana
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Cerca, Nuno
Martins, Silvia
Cerca, Filipe
Jefferson, Kimberly K.
Pier, Gerald B.
Oliveira, Rosário
Azeredo, Joana
dc.subject.por.fl_str_mv Nosocomial infections
Pathogens
Biofilms
Antibiotic resistance
CoNS
Science & Technology
topic Nosocomial infections
Pathogens
Biofilms
Antibiotic resistance
CoNS
Science & Technology
description Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/3648
url http://hdl.handle.net/1822/3648
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of antimicrobial chemotherapy". ISSN 0305-7453. 56:2 (2005) 331-336.
0305-7453
10.1093/jac/dki217
15980094
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132326070845440

Registros relacionados