Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal

Detalhes bibliográficos
Autor(a) principal: Dordio, Ana Mafalda
Data de Publicação: 2021
Outros Autores: Beck, Relja, Nunes, Telmo, Pereira da Fonseca, Isabel, Gomes, Jacinto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23319
Resumo: Research Areas: Parasitology ; Tropical Medicine
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spelling Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, PortugalCanine vector-borne diseases (CVBD)DogsMolecular methodsSouthern PortugalResearch Areas: Parasitology ; Tropical MedicineABSTRACT - Background: Canine vector-borne diseases (CVBDs) are caused by a wide range of pathogens transmitted by arthropods. They have been an issue of growing importance in recent years; however, there is limited information about the vector-borne pathogens circulating in Portugal. The aim of the present study was to detect canine vector-borne bacteria and protozoa of veterinary and zoonotic importance using molecular methods. Methods: One hundred and forty-two dogs from Lisbon, southern Portugal, were tested: 48 dogs from a veterinary hospital clinically suspected of vector-borne diseases and 94 apparently healthy dogs from shelters. Anaplasma spp./Ehrlichia spp., Babesia/Theileria spp., Hepatozoon spp., and Mycoplasma spp. infections were detected by PCR from blood samples and examined under light microscopy. Other information including clinical status and diagnostic test results were collected for each animal. Results: Infections were detected by PCR in 48 (33.80%) dogs. Single infections were found in 35 dogs (24.64%), and co-infections were found in 13 (9.15%) dogs. Twenty-nine (20.42%) dogs were positive for Hepatozoon spp., 15 (10.56%) for Mycoplasma spp., 11 (7.75%) for Anaplasma spp./Ehrlichia spp., and six (4.21%) for Babesia spp. DNA sequencing was used to identify Babesia vogeli (2.81%), Babesia canis (1.40%), Hepatozoon canis (20.42%), Mycoplasma haematoparvum (2.11%), Mycoplasma haemocanis (8.45%), Anaplasma platys (7.04%), and Ehrlichia canis (0.70%). Conclusions: This is the frst molecular identifcation of B. canis and M. haematoparvum in dogs from southern Portugal. This study highlights the importance of molecular methods to identify CVBD pathogens in endemic areas and helps to guide the clinical approach of veterinarians in practice.BMCRepositório da Universidade de LisboaDordio, Ana MafaldaBeck, ReljaNunes, TelmoPereira da Fonseca, IsabelGomes, Jacinto2022-01-30T23:20:43Z2021-03-182021-03-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23319engDordio AM, Beck R, Nunes T, da Fonseca IP, Gomes J. 2021. Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal. Parasites & Vectors. V. 14(1):163. Doi: 10.1186/s13071-021-04650-41756-330510.1186/s13071-021-04650-4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:50Zoai:www.repository.utl.pt:10400.5/23319Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:34.829452Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
title Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
spellingShingle Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
Dordio, Ana Mafalda
Canine vector-borne diseases (CVBD)
Dogs
Molecular methods
Southern Portugal
title_short Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
title_full Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
title_fullStr Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
title_full_unstemmed Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
title_sort Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
author Dordio, Ana Mafalda
author_facet Dordio, Ana Mafalda
Beck, Relja
Nunes, Telmo
Pereira da Fonseca, Isabel
Gomes, Jacinto
author_role author
author2 Beck, Relja
Nunes, Telmo
Pereira da Fonseca, Isabel
Gomes, Jacinto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Dordio, Ana Mafalda
Beck, Relja
Nunes, Telmo
Pereira da Fonseca, Isabel
Gomes, Jacinto
dc.subject.por.fl_str_mv Canine vector-borne diseases (CVBD)
Dogs
Molecular methods
Southern Portugal
topic Canine vector-borne diseases (CVBD)
Dogs
Molecular methods
Southern Portugal
description Research Areas: Parasitology ; Tropical Medicine
publishDate 2021
dc.date.none.fl_str_mv 2021-03-18
2021-03-18T00:00:00Z
2022-01-30T23:20:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23319
url http://hdl.handle.net/10400.5/23319
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dordio AM, Beck R, Nunes T, da Fonseca IP, Gomes J. 2021. Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal. Parasites & Vectors. V. 14(1):163. Doi: 10.1186/s13071-021-04650-4
1756-3305
10.1186/s13071-021-04650-4
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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