Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/23319 |
Resumo: | Research Areas: Parasitology ; Tropical Medicine |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, PortugalCanine vector-borne diseases (CVBD)DogsMolecular methodsSouthern PortugalResearch Areas: Parasitology ; Tropical MedicineABSTRACT - Background: Canine vector-borne diseases (CVBDs) are caused by a wide range of pathogens transmitted by arthropods. They have been an issue of growing importance in recent years; however, there is limited information about the vector-borne pathogens circulating in Portugal. The aim of the present study was to detect canine vector-borne bacteria and protozoa of veterinary and zoonotic importance using molecular methods. Methods: One hundred and forty-two dogs from Lisbon, southern Portugal, were tested: 48 dogs from a veterinary hospital clinically suspected of vector-borne diseases and 94 apparently healthy dogs from shelters. Anaplasma spp./Ehrlichia spp., Babesia/Theileria spp., Hepatozoon spp., and Mycoplasma spp. infections were detected by PCR from blood samples and examined under light microscopy. Other information including clinical status and diagnostic test results were collected for each animal. Results: Infections were detected by PCR in 48 (33.80%) dogs. Single infections were found in 35 dogs (24.64%), and co-infections were found in 13 (9.15%) dogs. Twenty-nine (20.42%) dogs were positive for Hepatozoon spp., 15 (10.56%) for Mycoplasma spp., 11 (7.75%) for Anaplasma spp./Ehrlichia spp., and six (4.21%) for Babesia spp. DNA sequencing was used to identify Babesia vogeli (2.81%), Babesia canis (1.40%), Hepatozoon canis (20.42%), Mycoplasma haematoparvum (2.11%), Mycoplasma haemocanis (8.45%), Anaplasma platys (7.04%), and Ehrlichia canis (0.70%). Conclusions: This is the frst molecular identifcation of B. canis and M. haematoparvum in dogs from southern Portugal. This study highlights the importance of molecular methods to identify CVBD pathogens in endemic areas and helps to guide the clinical approach of veterinarians in practice.BMCRepositório da Universidade de LisboaDordio, Ana MafaldaBeck, ReljaNunes, TelmoPereira da Fonseca, IsabelGomes, Jacinto2022-01-30T23:20:43Z2021-03-182021-03-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23319engDordio AM, Beck R, Nunes T, da Fonseca IP, Gomes J. 2021. Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal. Parasites & Vectors. V. 14(1):163. Doi: 10.1186/s13071-021-04650-41756-330510.1186/s13071-021-04650-4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:50Zoai:www.repository.utl.pt:10400.5/23319Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:34.829452Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal |
title |
Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal |
spellingShingle |
Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal Dordio, Ana Mafalda Canine vector-borne diseases (CVBD) Dogs Molecular methods Southern Portugal |
title_short |
Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal |
title_full |
Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal |
title_fullStr |
Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal |
title_full_unstemmed |
Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal |
title_sort |
Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal |
author |
Dordio, Ana Mafalda |
author_facet |
Dordio, Ana Mafalda Beck, Relja Nunes, Telmo Pereira da Fonseca, Isabel Gomes, Jacinto |
author_role |
author |
author2 |
Beck, Relja Nunes, Telmo Pereira da Fonseca, Isabel Gomes, Jacinto |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Dordio, Ana Mafalda Beck, Relja Nunes, Telmo Pereira da Fonseca, Isabel Gomes, Jacinto |
dc.subject.por.fl_str_mv |
Canine vector-borne diseases (CVBD) Dogs Molecular methods Southern Portugal |
topic |
Canine vector-borne diseases (CVBD) Dogs Molecular methods Southern Portugal |
description |
Research Areas: Parasitology ; Tropical Medicine |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-18 2021-03-18T00:00:00Z 2022-01-30T23:20:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/23319 |
url |
http://hdl.handle.net/10400.5/23319 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dordio AM, Beck R, Nunes T, da Fonseca IP, Gomes J. 2021. Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal. Parasites & Vectors. V. 14(1):163. Doi: 10.1186/s13071-021-04650-4 1756-3305 10.1186/s13071-021-04650-4 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
BMC |
publisher.none.fl_str_mv |
BMC |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131169960230912 |