Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic

Detalhes bibliográficos
Autor(a) principal: Monteiro, D. R.
Data de Publicação: 2014
Outros Autores: Takamiya, A. S., Feresin, L. P., Gorup, L. F., Camargo, E. R., Delbem, A. C. B., Henriques, Mariana, Barbosa, D. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/37171
Resumo: Our aim in this study was to evaluate how the chemical stability of silver nanoparticles (SNs) influences their efficacy against Candida albicans and C. glabrata biofilms. Several parameters of SN stability were tested, namely, temperature (50ºC, 70ºC, and 100ºC), pH (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. The control was defined as SNs without temperature treatment, pH 7, and 24 h of contact. These colloidal suspensions at 54 mg/L were used to treat mature Candida biofilms (48 h) formed on acrylic. Their efficacy was determined by total biomass and colony-forming unit quantification. Data were analyzed using analysis of variance and the Bonferroni post hoc test (=0.05). The temperature and pH variations of SNs did not affect their efficacy against the viable cells of Candida biofilms (P > 0.05). Moreover, the treatment periods were not decisive in terms of the susceptibility of Candida biofilms to SNs. These findings provide an important advantage of SNs that may be useful in the treatment of Candida-associated denture stomatitis.
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spelling Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylicSilver nanoparticlesChemical stabilityBiofilmsCandida albicansCandida glabrataScience & TechnologyOur aim in this study was to evaluate how the chemical stability of silver nanoparticles (SNs) influences their efficacy against Candida albicans and C. glabrata biofilms. Several parameters of SN stability were tested, namely, temperature (50ºC, 70ºC, and 100ºC), pH (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. The control was defined as SNs without temperature treatment, pH 7, and 24 h of contact. These colloidal suspensions at 54 mg/L were used to treat mature Candida biofilms (48 h) formed on acrylic. Their efficacy was determined by total biomass and colony-forming unit quantification. Data were analyzed using analysis of variance and the Bonferroni post hoc test (=0.05). The temperature and pH variations of SNs did not affect their efficacy against the viable cells of Candida biofilms (P > 0.05). Moreover, the treatment periods were not decisive in terms of the susceptibility of Candida biofilms to SNs. These findings provide an important advantage of SNs that may be useful in the treatment of Candida-associated denture stomatitis.We thank Dr David Williams, Cardiff University, Cardiff, UK, for providing the strain 324LA/94. The authors also thank Sao Paulo Research Foundation (FAPESP, process 2009/15146-5), Brazil, for supporting the work of D. R. M. The authors are indebted to Laboratorio Interdisciplinar de Eletroquimica e Ceramica, Federal University of Sao Carlos, Brazil, in the name of Andressa Kubo, for preparing and characterizing the colloidal suspensions of silver nanoparticles.Taylor and FrancisUniversidade do MinhoMonteiro, D. R.Takamiya, A. S.Feresin, L. P.Gorup, L. F.Camargo, E. R.Delbem, A. C. B.Henriques, MarianaBarbosa, D. B.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/37171engMonteiro, D.; Takamiya, A. S.; Feresin, L. P.; Gorup, L. F.; Camargo, E. R.; Delbem, A. C. B.; Henriques, Mariana; Barbosa, D. B., Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic. Medical Mycology, 52(6), 627-635, 20141369-37861460-270910.1093/mmy/myu02124951722info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:42:17Zoai:repositorium.sdum.uminho.pt:1822/37171Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:42:17Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic
title Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic
spellingShingle Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic
Monteiro, D. R.
Silver nanoparticles
Chemical stability
Biofilms
Candida albicans
Candida glabrata
Science & Technology
title_short Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic
title_full Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic
title_fullStr Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic
title_full_unstemmed Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic
title_sort Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic
author Monteiro, D. R.
author_facet Monteiro, D. R.
Takamiya, A. S.
Feresin, L. P.
Gorup, L. F.
Camargo, E. R.
Delbem, A. C. B.
Henriques, Mariana
Barbosa, D. B.
author_role author
author2 Takamiya, A. S.
Feresin, L. P.
Gorup, L. F.
Camargo, E. R.
Delbem, A. C. B.
Henriques, Mariana
Barbosa, D. B.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Monteiro, D. R.
Takamiya, A. S.
Feresin, L. P.
Gorup, L. F.
Camargo, E. R.
Delbem, A. C. B.
Henriques, Mariana
Barbosa, D. B.
dc.subject.por.fl_str_mv Silver nanoparticles
Chemical stability
Biofilms
Candida albicans
Candida glabrata
Science & Technology
topic Silver nanoparticles
Chemical stability
Biofilms
Candida albicans
Candida glabrata
Science & Technology
description Our aim in this study was to evaluate how the chemical stability of silver nanoparticles (SNs) influences their efficacy against Candida albicans and C. glabrata biofilms. Several parameters of SN stability were tested, namely, temperature (50ºC, 70ºC, and 100ºC), pH (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. The control was defined as SNs without temperature treatment, pH 7, and 24 h of contact. These colloidal suspensions at 54 mg/L were used to treat mature Candida biofilms (48 h) formed on acrylic. Their efficacy was determined by total biomass and colony-forming unit quantification. Data were analyzed using analysis of variance and the Bonferroni post hoc test (=0.05). The temperature and pH variations of SNs did not affect their efficacy against the viable cells of Candida biofilms (P > 0.05). Moreover, the treatment periods were not decisive in terms of the susceptibility of Candida biofilms to SNs. These findings provide an important advantage of SNs that may be useful in the treatment of Candida-associated denture stomatitis.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/37171
url http://hdl.handle.net/1822/37171
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Monteiro, D.; Takamiya, A. S.; Feresin, L. P.; Gorup, L. F.; Camargo, E. R.; Delbem, A. C. B.; Henriques, Mariana; Barbosa, D. B., Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic. Medical Mycology, 52(6), 627-635, 2014
1369-3786
1460-2709
10.1093/mmy/myu021
24951722
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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