Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/37171 |
Resumo: | Our aim in this study was to evaluate how the chemical stability of silver nanoparticles (SNs) influences their efficacy against Candida albicans and C. glabrata biofilms. Several parameters of SN stability were tested, namely, temperature (50ºC, 70ºC, and 100ºC), pH (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. The control was defined as SNs without temperature treatment, pH 7, and 24 h of contact. These colloidal suspensions at 54 mg/L were used to treat mature Candida biofilms (48 h) formed on acrylic. Their efficacy was determined by total biomass and colony-forming unit quantification. Data were analyzed using analysis of variance and the Bonferroni post hoc test (=0.05). The temperature and pH variations of SNs did not affect their efficacy against the viable cells of Candida biofilms (P > 0.05). Moreover, the treatment periods were not decisive in terms of the susceptibility of Candida biofilms to SNs. These findings provide an important advantage of SNs that may be useful in the treatment of Candida-associated denture stomatitis. |
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Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylicSilver nanoparticlesChemical stabilityBiofilmsCandida albicansCandida glabrataScience & TechnologyOur aim in this study was to evaluate how the chemical stability of silver nanoparticles (SNs) influences their efficacy against Candida albicans and C. glabrata biofilms. Several parameters of SN stability were tested, namely, temperature (50ºC, 70ºC, and 100ºC), pH (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. The control was defined as SNs without temperature treatment, pH 7, and 24 h of contact. These colloidal suspensions at 54 mg/L were used to treat mature Candida biofilms (48 h) formed on acrylic. Their efficacy was determined by total biomass and colony-forming unit quantification. Data were analyzed using analysis of variance and the Bonferroni post hoc test (=0.05). The temperature and pH variations of SNs did not affect their efficacy against the viable cells of Candida biofilms (P > 0.05). Moreover, the treatment periods were not decisive in terms of the susceptibility of Candida biofilms to SNs. These findings provide an important advantage of SNs that may be useful in the treatment of Candida-associated denture stomatitis.We thank Dr David Williams, Cardiff University, Cardiff, UK, for providing the strain 324LA/94. The authors also thank Sao Paulo Research Foundation (FAPESP, process 2009/15146-5), Brazil, for supporting the work of D. R. M. The authors are indebted to Laboratorio Interdisciplinar de Eletroquimica e Ceramica, Federal University of Sao Carlos, Brazil, in the name of Andressa Kubo, for preparing and characterizing the colloidal suspensions of silver nanoparticles.Taylor and FrancisUniversidade do MinhoMonteiro, D. R.Takamiya, A. S.Feresin, L. P.Gorup, L. F.Camargo, E. R.Delbem, A. C. B.Henriques, MarianaBarbosa, D. B.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/37171engMonteiro, D.; Takamiya, A. S.; Feresin, L. P.; Gorup, L. F.; Camargo, E. R.; Delbem, A. C. B.; Henriques, Mariana; Barbosa, D. B., Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic. Medical Mycology, 52(6), 627-635, 20141369-37861460-270910.1093/mmy/myu02124951722info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:42:17Zoai:repositorium.sdum.uminho.pt:1822/37171Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:42:17Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic |
title |
Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic |
spellingShingle |
Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic Monteiro, D. R. Silver nanoparticles Chemical stability Biofilms Candida albicans Candida glabrata Science & Technology |
title_short |
Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic |
title_full |
Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic |
title_fullStr |
Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic |
title_full_unstemmed |
Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic |
title_sort |
Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic |
author |
Monteiro, D. R. |
author_facet |
Monteiro, D. R. Takamiya, A. S. Feresin, L. P. Gorup, L. F. Camargo, E. R. Delbem, A. C. B. Henriques, Mariana Barbosa, D. B. |
author_role |
author |
author2 |
Takamiya, A. S. Feresin, L. P. Gorup, L. F. Camargo, E. R. Delbem, A. C. B. Henriques, Mariana Barbosa, D. B. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Monteiro, D. R. Takamiya, A. S. Feresin, L. P. Gorup, L. F. Camargo, E. R. Delbem, A. C. B. Henriques, Mariana Barbosa, D. B. |
dc.subject.por.fl_str_mv |
Silver nanoparticles Chemical stability Biofilms Candida albicans Candida glabrata Science & Technology |
topic |
Silver nanoparticles Chemical stability Biofilms Candida albicans Candida glabrata Science & Technology |
description |
Our aim in this study was to evaluate how the chemical stability of silver nanoparticles (SNs) influences their efficacy against Candida albicans and C. glabrata biofilms. Several parameters of SN stability were tested, namely, temperature (50ºC, 70ºC, and 100ºC), pH (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. The control was defined as SNs without temperature treatment, pH 7, and 24 h of contact. These colloidal suspensions at 54 mg/L were used to treat mature Candida biofilms (48 h) formed on acrylic. Their efficacy was determined by total biomass and colony-forming unit quantification. Data were analyzed using analysis of variance and the Bonferroni post hoc test (=0.05). The temperature and pH variations of SNs did not affect their efficacy against the viable cells of Candida biofilms (P > 0.05). Moreover, the treatment periods were not decisive in terms of the susceptibility of Candida biofilms to SNs. These findings provide an important advantage of SNs that may be useful in the treatment of Candida-associated denture stomatitis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/37171 |
url |
http://hdl.handle.net/1822/37171 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Monteiro, D.; Takamiya, A. S.; Feresin, L. P.; Gorup, L. F.; Camargo, E. R.; Delbem, A. C. B.; Henriques, Mariana; Barbosa, D. B., Silver colloidal nanoparticle stability: influence on Candida biofilms formed on denture acrylic. Medical Mycology, 52(6), 627-635, 2014 1369-3786 1460-2709 10.1093/mmy/myu021 24951722 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Taylor and Francis |
publisher.none.fl_str_mv |
Taylor and Francis |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545063445561344 |