Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Sousa, C. A.
Data de Publicação: 2019
Outros Autores: Soares, Helena M. V. M., Soares, Eduardo V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/59341
Resumo: The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.chemrestox.8b00265. Comparison of the evolution of cell death, plasma membrane integrity, and double staining [DiOC6(3) and propidium iodide] of S. cerevisiae BY4741 exposed to NiO (PDF)
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spelling Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiaeScience & TechnologyThe Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.chemrestox.8b00265. Comparison of the evolution of cell death, plasma membrane integrity, and double staining [DiOC6(3) and propidium iodide] of S. cerevisiae BY4741 exposed to NiO (PDF)The expansion of industrial use of nickel oxide (NiO) nanoparticles (NPs) raises concerns about their potential adverse effects. The present work aimed to investigate the mechanisms of toxicity induced by NiO NPs, using the yeast Saccharomyces cerevisiae, as cell model. Yeast cells exposed to NiO NPs exhibited typical hallmarks of regulated cell death (RCD) by apoptosis [loss of cell proliferation capacity (cell viability), exposure of phosphatidylserine at the outer cytoplasmic membrane leaflet, nuclear chromatin condensation and DNA damage] in a process which required de novo protein synthesis. The execution of yeast cell death induced by NiO NPs is Yca1p metacaspase-dependent. NiO NPs also induced the reduction of the mitochondrial membrane potential and the increase of the frequency of respiratory deficient mutants, which supports the involvement of mitochondria in the cell death process. Cells deficient in the apoptosis-inducing factor (aif1) displayed higher tolerance to NiO NPs, which reinforces the involvement of mitochondria in the RCD by apoptosis. In resume, this study shows that NiO NPs induce caspase- and mitochondria-dependent apoptosis in yeast. The presented results warn to the possible harmful effects associated with the use of NiO NPs.This work was performed in the framework of the financing by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145FEDER-006684), and BioTecNorte operation (NORTE-010145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020Programa Operacional Regional do Norte and LAQV (UID/QUI/50006/2013-POCI/01/0145/FEDER/007265) with financial support from FCT/MEC through national funds and cofinanced by FEDER, under Partnership Agreement PT2020. C.A.S. gratefully acknowledges the doctoral grant (SFRH/BD/101452/2014) from FCT.info:eu-repo/semantics/publishedVersionAmerican Chemical SocietyUniversidade do MinhoSousa, C. A.Soares, Helena M. V. M.Soares, Eduardo V.2019-01-182019-01-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/59341engSousa, C. A.; Soares, Helena M. V. M.; Soares, Eduardo V., Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae. Chemical Research in Toxicology, 32(2), 245-254, 20190893-228X1520-501010.1021/acs.chemrestox.8b0026530656935https://pubs.acs.org/journal/crtoecinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:55Zoai:repositorium.sdum.uminho.pt:1822/59341Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:25.470671Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae
title Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae
spellingShingle Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae
Sousa, C. A.
Science & Technology
title_short Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae
title_full Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae
title_fullStr Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae
title_full_unstemmed Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae
title_sort Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae
author Sousa, C. A.
author_facet Sousa, C. A.
Soares, Helena M. V. M.
Soares, Eduardo V.
author_role author
author2 Soares, Helena M. V. M.
Soares, Eduardo V.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sousa, C. A.
Soares, Helena M. V. M.
Soares, Eduardo V.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.chemrestox.8b00265. Comparison of the evolution of cell death, plasma membrane integrity, and double staining [DiOC6(3) and propidium iodide] of S. cerevisiae BY4741 exposed to NiO (PDF)
publishDate 2019
dc.date.none.fl_str_mv 2019-01-18
2019-01-18T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/59341
url http://hdl.handle.net/1822/59341
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sousa, C. A.; Soares, Helena M. V. M.; Soares, Eduardo V., Nickel oxide nanoparticles trigger caspase- and mitochondria-dependent apoptosis in the yeast Saccharomyces cerevisiae. Chemical Research in Toxicology, 32(2), 245-254, 2019
0893-228X
1520-5010
10.1021/acs.chemrestox.8b00265
30656935
https://pubs.acs.org/journal/crtoec
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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