Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/10212 |
Resumo: | Matrix-assisted laser desorption ⁄ ionization time-of-flight intact cell mass spectrometry (MALDI-TOF ICMS) is coming of age for the identification and characterization of fungi. The procedure has been used extensively with bacteria. UV-absorbing matrices function as energy mediators that transfer the absorbed photoenergy from an irradiation source to the surrounding sample molecules, resulting in minimum fragmentation. A surprisingly high number of fungal groups have been studied: (i) the terverticillate penicillia, (ii) aflatoxigenic, black and other aspergilli, (iii) Fusarium, (iv) Trichoderma, (iv) wood rotting fungi (e.g. Serpula lacrymans) and (v) dermatophytes. The technique has been suggested for optimizing quality control of fungal Chinese medicines (e.g. Cordyceps). MALDI-TOF ICMS offers advantages over PCR. The method is now used in taxonomic assessments (e.g. Trichoderma) as distinct from only strain characterization. Low and high molecular mass natural products (e.g. peptaibols) can be analysed. The procedure is rapid and requires minimal pretreatment. However, issues of reproducibility need to be addressed further in terms of strains of species tested and between run variability. More studies into the capabilities of MALDI-TOF ICMS to identify fungi are required. |
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Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometryFilamentous fungiFungal identificationMALDI-TOF ICMSRapid techniquesSpectral analysisScience & TechnologyMatrix-assisted laser desorption ⁄ ionization time-of-flight intact cell mass spectrometry (MALDI-TOF ICMS) is coming of age for the identification and characterization of fungi. The procedure has been used extensively with bacteria. UV-absorbing matrices function as energy mediators that transfer the absorbed photoenergy from an irradiation source to the surrounding sample molecules, resulting in minimum fragmentation. A surprisingly high number of fungal groups have been studied: (i) the terverticillate penicillia, (ii) aflatoxigenic, black and other aspergilli, (iii) Fusarium, (iv) Trichoderma, (iv) wood rotting fungi (e.g. Serpula lacrymans) and (v) dermatophytes. The technique has been suggested for optimizing quality control of fungal Chinese medicines (e.g. Cordyceps). MALDI-TOF ICMS offers advantages over PCR. The method is now used in taxonomic assessments (e.g. Trichoderma) as distinct from only strain characterization. Low and high molecular mass natural products (e.g. peptaibols) can be analysed. The procedure is rapid and requires minimal pretreatment. However, issues of reproducibility need to be addressed further in terms of strains of species tested and between run variability. More studies into the capabilities of MALDI-TOF ICMS to identify fungi are required.R.R.M. Paterson is grateful for FCT-Portugal Grant (SFRH/BPD/34879/2007) and IOI Professorial Chair, Malaysia where some of this review was first revised. C. Santos acknowledges FACEPE/CNPq-Brazil financial support. N. Lima appreciates the stay in the Biochemistry Department of UFPE-Brazil as Visiting Full Professor where this review was drafted. C. Santos and N. Lima acknowledge Laboratorio de Imunopatologia Keizo Asami (LIKA/UFPE) for the consent and support given to use their MALDI-TOF MS equipment.Wiley-BlackwellUniversidade do MinhoSantos, C.Paterson, R. R. M.Venâncio, ArmandoLima, Nelson2010-022010-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/10212eng"Journal of Applied Microbiology". ISSN 1364-5072. 108:2 (Febr. 2010) 375-385.1364-507210.1111/j.1365-2672.2009.04448.x19659699info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:21:45Zoai:repositorium.sdum.uminho.pt:1822/10212Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:21:45Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title |
Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
spellingShingle |
Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Santos, C. Filamentous fungi Fungal identification MALDI-TOF ICMS Rapid techniques Spectral analysis Science & Technology |
title_short |
Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_full |
Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_fullStr |
Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_full_unstemmed |
Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_sort |
Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
author |
Santos, C. |
author_facet |
Santos, C. Paterson, R. R. M. Venâncio, Armando Lima, Nelson |
author_role |
author |
author2 |
Paterson, R. R. M. Venâncio, Armando Lima, Nelson |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, C. Paterson, R. R. M. Venâncio, Armando Lima, Nelson |
dc.subject.por.fl_str_mv |
Filamentous fungi Fungal identification MALDI-TOF ICMS Rapid techniques Spectral analysis Science & Technology |
topic |
Filamentous fungi Fungal identification MALDI-TOF ICMS Rapid techniques Spectral analysis Science & Technology |
description |
Matrix-assisted laser desorption ⁄ ionization time-of-flight intact cell mass spectrometry (MALDI-TOF ICMS) is coming of age for the identification and characterization of fungi. The procedure has been used extensively with bacteria. UV-absorbing matrices function as energy mediators that transfer the absorbed photoenergy from an irradiation source to the surrounding sample molecules, resulting in minimum fragmentation. A surprisingly high number of fungal groups have been studied: (i) the terverticillate penicillia, (ii) aflatoxigenic, black and other aspergilli, (iii) Fusarium, (iv) Trichoderma, (iv) wood rotting fungi (e.g. Serpula lacrymans) and (v) dermatophytes. The technique has been suggested for optimizing quality control of fungal Chinese medicines (e.g. Cordyceps). MALDI-TOF ICMS offers advantages over PCR. The method is now used in taxonomic assessments (e.g. Trichoderma) as distinct from only strain characterization. Low and high molecular mass natural products (e.g. peptaibols) can be analysed. The procedure is rapid and requires minimal pretreatment. However, issues of reproducibility need to be addressed further in terms of strains of species tested and between run variability. More studies into the capabilities of MALDI-TOF ICMS to identify fungi are required. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02 2010-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/10212 |
url |
http://hdl.handle.net/1822/10212 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of Applied Microbiology". ISSN 1364-5072. 108:2 (Febr. 2010) 375-385. 1364-5072 10.1111/j.1365-2672.2009.04448.x 19659699 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817544597107113984 |