Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Physics |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-97332006000500037 |
Resumo: | A matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer has been built with the capabilities to perform structural analyzes. The first time-of-flight mass spectrometer includes a timing ion gate to select the ions to be analyzed. After the selection process, the ions are activated using an electrically floating collision cell. The fragments produced in the collision cell are analyzed in the second time-of-flight stage. A two-step mass calibration procedure is introduced that allows one to obtain accurate mass values. The characteristics of this calibration procedure make possible to automate it. The experimental results of the tandem mass spectrometer and calibration procedure using different standard peptides are presented. |
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Brazilian Journal of Physics |
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Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometerTadem mass spectrometryTime-of-flightTOF/TOFMS/MSMALDIAutomationMass calibrationA matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer has been built with the capabilities to perform structural analyzes. The first time-of-flight mass spectrometer includes a timing ion gate to select the ions to be analyzed. After the selection process, the ions are activated using an electrically floating collision cell. The fragments produced in the collision cell are analyzed in the second time-of-flight stage. A two-step mass calibration procedure is introduced that allows one to obtain accurate mass values. The characteristics of this calibration procedure make possible to automate it. The experimental results of the tandem mass spectrometer and calibration procedure using different standard peptides are presented.Sociedade Brasileira de Física2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-97332006000500037Brazilian Journal of Physics v.36 n.3a 2006reponame:Brazilian Journal of Physicsinstname:Sociedade Brasileira de Física (SBF)instacron:SBF10.1590/S0103-97332006000500037info:eu-repo/semantics/openAccessSantacruz,C. P.Ayala,E.Costa-Vera,C.eng2006-10-16T00:00:00Zoai:scielo:S0103-97332006000500037Revistahttp://www.sbfisica.org.br/v1/home/index.php/pt/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbfisica@sbfisica.org.br||sbfisica@sbfisica.org.br1678-44480103-9733opendoar:2006-10-16T00:00Brazilian Journal of Physics - Sociedade Brasileira de Física (SBF)false |
dc.title.none.fl_str_mv |
Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer |
title |
Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer |
spellingShingle |
Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer Santacruz,C. P. Tadem mass spectrometry Time-of-flight TOF/TOF MS/MS MALDI Automation Mass calibration |
title_short |
Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer |
title_full |
Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer |
title_fullStr |
Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer |
title_full_unstemmed |
Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer |
title_sort |
Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer |
author |
Santacruz,C. P. |
author_facet |
Santacruz,C. P. Ayala,E. Costa-Vera,C. |
author_role |
author |
author2 |
Ayala,E. Costa-Vera,C. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Santacruz,C. P. Ayala,E. Costa-Vera,C. |
dc.subject.por.fl_str_mv |
Tadem mass spectrometry Time-of-flight TOF/TOF MS/MS MALDI Automation Mass calibration |
topic |
Tadem mass spectrometry Time-of-flight TOF/TOF MS/MS MALDI Automation Mass calibration |
description |
A matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer has been built with the capabilities to perform structural analyzes. The first time-of-flight mass spectrometer includes a timing ion gate to select the ions to be analyzed. After the selection process, the ions are activated using an electrically floating collision cell. The fragments produced in the collision cell are analyzed in the second time-of-flight stage. A two-step mass calibration procedure is introduced that allows one to obtain accurate mass values. The characteristics of this calibration procedure make possible to automate it. The experimental results of the tandem mass spectrometer and calibration procedure using different standard peptides are presented. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-97332006000500037 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-97332006000500037 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-97332006000500037 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Física |
publisher.none.fl_str_mv |
Sociedade Brasileira de Física |
dc.source.none.fl_str_mv |
Brazilian Journal of Physics v.36 n.3a 2006 reponame:Brazilian Journal of Physics instname:Sociedade Brasileira de Física (SBF) instacron:SBF |
instname_str |
Sociedade Brasileira de Física (SBF) |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Brazilian Journal of Physics |
collection |
Brazilian Journal of Physics |
repository.name.fl_str_mv |
Brazilian Journal of Physics - Sociedade Brasileira de Física (SBF) |
repository.mail.fl_str_mv |
sbfisica@sbfisica.org.br||sbfisica@sbfisica.org.br |
_version_ |
1754734863077867520 |