Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer

Detalhes bibliográficos
Autor(a) principal: Santacruz,C. P.
Data de Publicação: 2006
Outros Autores: Ayala,E., Costa-Vera,C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Physics
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-97332006000500037
Resumo: A matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer has been built with the capabilities to perform structural analyzes. The first time-of-flight mass spectrometer includes a timing ion gate to select the ions to be analyzed. After the selection process, the ions are activated using an electrically floating collision cell. The fragments produced in the collision cell are analyzed in the second time-of-flight stage. A two-step mass calibration procedure is introduced that allows one to obtain accurate mass values. The characteristics of this calibration procedure make possible to automate it. The experimental results of the tandem mass spectrometer and calibration procedure using different standard peptides are presented.
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spelling Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometerTadem mass spectrometryTime-of-flightTOF/TOFMS/MSMALDIAutomationMass calibrationA matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer has been built with the capabilities to perform structural analyzes. The first time-of-flight mass spectrometer includes a timing ion gate to select the ions to be analyzed. After the selection process, the ions are activated using an electrically floating collision cell. The fragments produced in the collision cell are analyzed in the second time-of-flight stage. A two-step mass calibration procedure is introduced that allows one to obtain accurate mass values. The characteristics of this calibration procedure make possible to automate it. The experimental results of the tandem mass spectrometer and calibration procedure using different standard peptides are presented.Sociedade Brasileira de Física2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-97332006000500037Brazilian Journal of Physics v.36 n.3a 2006reponame:Brazilian Journal of Physicsinstname:Sociedade Brasileira de Física (SBF)instacron:SBF10.1590/S0103-97332006000500037info:eu-repo/semantics/openAccessSantacruz,C. P.Ayala,E.Costa-Vera,C.eng2006-10-16T00:00:00Zoai:scielo:S0103-97332006000500037Revistahttp://www.sbfisica.org.br/v1/home/index.php/pt/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbfisica@sbfisica.org.br||sbfisica@sbfisica.org.br1678-44480103-9733opendoar:2006-10-16T00:00Brazilian Journal of Physics - Sociedade Brasileira de Física (SBF)false
dc.title.none.fl_str_mv Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
title Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
spellingShingle Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
Santacruz,C. P.
Tadem mass spectrometry
Time-of-flight
TOF/TOF
MS/MS
MALDI
Automation
Mass calibration
title_short Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
title_full Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
title_fullStr Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
title_full_unstemmed Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
title_sort Design and performance of a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
author Santacruz,C. P.
author_facet Santacruz,C. P.
Ayala,E.
Costa-Vera,C.
author_role author
author2 Ayala,E.
Costa-Vera,C.
author2_role author
author
dc.contributor.author.fl_str_mv Santacruz,C. P.
Ayala,E.
Costa-Vera,C.
dc.subject.por.fl_str_mv Tadem mass spectrometry
Time-of-flight
TOF/TOF
MS/MS
MALDI
Automation
Mass calibration
topic Tadem mass spectrometry
Time-of-flight
TOF/TOF
MS/MS
MALDI
Automation
Mass calibration
description A matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer has been built with the capabilities to perform structural analyzes. The first time-of-flight mass spectrometer includes a timing ion gate to select the ions to be analyzed. After the selection process, the ions are activated using an electrically floating collision cell. The fragments produced in the collision cell are analyzed in the second time-of-flight stage. A two-step mass calibration procedure is introduced that allows one to obtain accurate mass values. The characteristics of this calibration procedure make possible to automate it. The experimental results of the tandem mass spectrometer and calibration procedure using different standard peptides are presented.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-97332006000500037
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-97332006000500037
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-97332006000500037
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Física
publisher.none.fl_str_mv Sociedade Brasileira de Física
dc.source.none.fl_str_mv Brazilian Journal of Physics v.36 n.3a 2006
reponame:Brazilian Journal of Physics
instname:Sociedade Brasileira de Física (SBF)
instacron:SBF
instname_str Sociedade Brasileira de Física (SBF)
instacron_str SBF
institution SBF
reponame_str Brazilian Journal of Physics
collection Brazilian Journal of Physics
repository.name.fl_str_mv Brazilian Journal of Physics - Sociedade Brasileira de Física (SBF)
repository.mail.fl_str_mv sbfisica@sbfisica.org.br||sbfisica@sbfisica.org.br
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