Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Rui S.
Data de Publicação: 2017
Outros Autores: Ribeiro, Filipa, Ferreira, Filipa, Vaz, Sandra H., Sebastião, Ana M, Xapelli, Sara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49125
Resumo: Copyright © 2017 Rodrigues, Ribeiro, Ferreira, Vaz, Sebastião and Xapelli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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spelling Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesisCannabinoid type 1 receptorCannabinoid type 2 receptorDentate gyrusPostnatal neurogenesisSubventricular zoneCopyright © 2017 Rodrigues, Ribeiro, Ferreira, Vaz, Sebastião and Xapelli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Neurogenesis in the adult mammalian brain occurs mainly in two neurogenic niches, the subventricular zone (SVZ) and the subgranular zone (SGZ) of the dentate gyrus (DG). Cannabinoid type 1 and 2 receptors (CB1R and CB2R) have been shown to differently modulate neurogenesis. However, low attention has been given to the interaction between CB1R and CB2R in modulating postnatal neurogenesis (proliferation, neuronal differentiation and maturation). We focused on a putative crosstalk between CB1R and CB2R to modulate neurogenesis and cultured SVZ and DG stem/progenitor cells from early postnatal (P1-3) Sprague-Dawley rats. Data showed that the non-selective cannabinoid receptor agonist WIN55,212-2 promotes DG cell proliferation (measured by BrdU staining), an effect blocked by either CB1R or CB2R selective antagonists. Experiments with selective agonists showed that facilitation of DG cell proliferation requires co-activation of both CB1R and CB2R. Cell proliferation in the SVZ was not affected by the non-selective receptor agonist, but it was enhanced by CB1R selective activation. However, either CB1R or CB2R selective antagonists abolished the effect of the CB1R agonist in SVZ cell proliferation. Neuronal differentiation (measured by immunocytochemistry against neuronal markers of different stages and calcium imaging) was facilitated by WIN55,212-2 at both SVZ and DG. This effect was mimicked by either CB1R or CB2R selective agonists and blocked by either CB1R or CB2R selective antagonists, cross-antagonism being evident. In summary, our findings indicate a tight interaction between CB1R and CB2R to modulate neurogenesis in the two major neurogenic niches, thus contributing to further unraveling the mechanisms behind the action of endocannabinoids in the brain.This work was supported by LISBOA-01-0145-FEDER-007391, project co-funded by FEDER through POR Lisboa 2020 (Programa Operacional Regional de Lisboa) from PORTUGAL 2020, and by Fundação para a Ciência e a Tecnologia (FCT). AS thanks the following supports: PTDC/DTP-FTO/3346/2014 from FCT and H2020 Twinning Action from EU (SynaNet 692340). SX is grateful for the support by the COST action BM1402. RR (IMM/BI/42-2016), FR (SFRH/BD/74662/2010), SV (SFRH/BPD/81627/2011), and SX (SFRH/BPD/76642/2011 and IF/01227/2015) were in receipt of a fellowship from FCT.FrontiersRepositório da Universidade de LisboaRodrigues, Rui S.Ribeiro, FilipaFerreira, FilipaVaz, Sandra H.Sebastião, Ana MXapelli, Sara2021-07-26T14:55:59Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49125engFront Pharmacol. 2017 Aug 10;8:516.10.3389/fphar.2017.005161663-9812info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:52:32Zoai:repositorio.ul.pt:10451/49125Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:42.393062Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis
title Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis
spellingShingle Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis
Rodrigues, Rui S.
Cannabinoid type 1 receptor
Cannabinoid type 2 receptor
Dentate gyrus
Postnatal neurogenesis
Subventricular zone
title_short Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis
title_full Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis
title_fullStr Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis
title_full_unstemmed Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis
title_sort Interaction between cannabinoid type 1 and type 2 receptors in the modulation of subventricular zone and dentate Gyrus neurogenesis
author Rodrigues, Rui S.
author_facet Rodrigues, Rui S.
Ribeiro, Filipa
Ferreira, Filipa
Vaz, Sandra H.
Sebastião, Ana M
Xapelli, Sara
author_role author
author2 Ribeiro, Filipa
Ferreira, Filipa
Vaz, Sandra H.
Sebastião, Ana M
Xapelli, Sara
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rodrigues, Rui S.
Ribeiro, Filipa
Ferreira, Filipa
Vaz, Sandra H.
Sebastião, Ana M
Xapelli, Sara
dc.subject.por.fl_str_mv Cannabinoid type 1 receptor
Cannabinoid type 2 receptor
Dentate gyrus
Postnatal neurogenesis
Subventricular zone
topic Cannabinoid type 1 receptor
Cannabinoid type 2 receptor
Dentate gyrus
Postnatal neurogenesis
Subventricular zone
description Copyright © 2017 Rodrigues, Ribeiro, Ferreira, Vaz, Sebastião and Xapelli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2021-07-26T14:55:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49125
url http://hdl.handle.net/10451/49125
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Front Pharmacol. 2017 Aug 10;8:516.
10.3389/fphar.2017.00516
1663-9812
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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