Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals

Detalhes bibliográficos
Autor(a) principal: Lawal, Opeyemi U.
Data de Publicação: 2021
Outros Autores: Fraqueza, M. J., Worning, Peder, Bouchami, Ons
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23413
Resumo: Research Areas: Microbiology ; Pharmacology & Pharmacy
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spelling Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metalsStaphylococcus saprophyticusUrinary tract infectionEnvironmentHeavy metalsWhole-genome sequencingPan-GWASMetal resistance determinantsCopperZincArsenicCadmiumMetal resistanceResearch Areas: Microbiology ; Pharmacology & PharmacyStaphylococcus saprophyticus is a common pathogen of the urinary tract, a heavy metal-rich environment, but information regarding its heavy metal resistance is unknown. We investigated 422 S. saprophyticus isolates from human infection and colonization/contamination, animals, and environmental sources for resistance to copper, zinc, arsenic, and cadmium using the agar dilution method. To identify the genes associated with metal resistance and assess possible links to pathogenicity, we accessed the wholegenome sequence of all isolates and used in silico and pangenome-wide association approaches. The MIC values for copper and zinc were uniformly high (1,600mg/liter). Genes encoding copper efflux pumps (copA, copB, copZ, mco, and csoR) and zinc transporters (zinT, czrAB, znuBC, and zur) were abundant in the population (20 to 100%). Arsenic and cadmium showed various susceptibility levels. Genes encoding the ars operon (arsRDABC), an ABC transporter and a two-component permease, were linked to resistance to arsenic (MICs$ 1,600mg/liter; 14% [58/422]; P, 0.05). At least three cad genes (cadA or cadC and cadD-cadX or czrC) and genes encoding multidrug efflux pumps and hyperosmoregulation in acidified conditions were associated with resistance to cadmium (MICs$ 200mg/liter; 20% [85/422]; P, 0.05). These resistance genes were frequently carried by mobile genetic elements. Resistance to arsenic and cadmium were linked to human infection and a clonal lineage originating in animals (P, 0.05). Altogether, S. saprophyticus was highly resistant to heavy metals and accumulated multiple metal resistance determinants. The highest arsenic and cadmium resistance levels were associated with infection, suggesting resistance to these metals is relevant for S. saprophyticus pathogenicityAmerican Society for MicrobiologyRepositório da Universidade de LisboaLawal, Opeyemi U.Fraqueza, M. J.Worning, PederBouchami, Ons2022-02-06T22:43:12Z2021-06-172021-06-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23413engLawal OU, Fraqueza MJ, Worning P, Bouchami O, Bartels MD, Goncalves L, Paixão P, Goncalves E, Toscano C, Empel J, Urbans M, Domínguez MA, Westh H, de Lencastre H, Miragaia M. 2021. Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals. Antimicrobial Agents Chemotherapy, 65(7):e02685-20. Doi: 10.1128/AAC.02685-200066-480410.1128/AAC.02685-201098-6596info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:58Zoai:www.repository.utl.pt:10400.5/23413Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:39.181473Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals
title Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals
spellingShingle Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals
Lawal, Opeyemi U.
Staphylococcus saprophyticus
Urinary tract infection
Environment
Heavy metals
Whole-genome sequencing
Pan-GWAS
Metal resistance determinants
Copper
Zinc
Arsenic
Cadmium
Metal resistance
title_short Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals
title_full Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals
title_fullStr Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals
title_full_unstemmed Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals
title_sort Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals
author Lawal, Opeyemi U.
author_facet Lawal, Opeyemi U.
Fraqueza, M. J.
Worning, Peder
Bouchami, Ons
author_role author
author2 Fraqueza, M. J.
Worning, Peder
Bouchami, Ons
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Lawal, Opeyemi U.
Fraqueza, M. J.
Worning, Peder
Bouchami, Ons
dc.subject.por.fl_str_mv Staphylococcus saprophyticus
Urinary tract infection
Environment
Heavy metals
Whole-genome sequencing
Pan-GWAS
Metal resistance determinants
Copper
Zinc
Arsenic
Cadmium
Metal resistance
topic Staphylococcus saprophyticus
Urinary tract infection
Environment
Heavy metals
Whole-genome sequencing
Pan-GWAS
Metal resistance determinants
Copper
Zinc
Arsenic
Cadmium
Metal resistance
description Research Areas: Microbiology ; Pharmacology & Pharmacy
publishDate 2021
dc.date.none.fl_str_mv 2021-06-17
2021-06-17T00:00:00Z
2022-02-06T22:43:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23413
url http://hdl.handle.net/10400.5/23413
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lawal OU, Fraqueza MJ, Worning P, Bouchami O, Bartels MD, Goncalves L, Paixão P, Goncalves E, Toscano C, Empel J, Urbans M, Domínguez MA, Westh H, de Lencastre H, Miragaia M. 2021. Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals. Antimicrobial Agents Chemotherapy, 65(7):e02685-20. Doi: 10.1128/AAC.02685-20
0066-4804
10.1128/AAC.02685-20
1098-6596
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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