Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/23413 |
Resumo: | Research Areas: Microbiology ; Pharmacology & Pharmacy |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metalsStaphylococcus saprophyticusUrinary tract infectionEnvironmentHeavy metalsWhole-genome sequencingPan-GWASMetal resistance determinantsCopperZincArsenicCadmiumMetal resistanceResearch Areas: Microbiology ; Pharmacology & PharmacyStaphylococcus saprophyticus is a common pathogen of the urinary tract, a heavy metal-rich environment, but information regarding its heavy metal resistance is unknown. We investigated 422 S. saprophyticus isolates from human infection and colonization/contamination, animals, and environmental sources for resistance to copper, zinc, arsenic, and cadmium using the agar dilution method. To identify the genes associated with metal resistance and assess possible links to pathogenicity, we accessed the wholegenome sequence of all isolates and used in silico and pangenome-wide association approaches. The MIC values for copper and zinc were uniformly high (1,600mg/liter). Genes encoding copper efflux pumps (copA, copB, copZ, mco, and csoR) and zinc transporters (zinT, czrAB, znuBC, and zur) were abundant in the population (20 to 100%). Arsenic and cadmium showed various susceptibility levels. Genes encoding the ars operon (arsRDABC), an ABC transporter and a two-component permease, were linked to resistance to arsenic (MICs$ 1,600mg/liter; 14% [58/422]; P, 0.05). At least three cad genes (cadA or cadC and cadD-cadX or czrC) and genes encoding multidrug efflux pumps and hyperosmoregulation in acidified conditions were associated with resistance to cadmium (MICs$ 200mg/liter; 20% [85/422]; P, 0.05). These resistance genes were frequently carried by mobile genetic elements. Resistance to arsenic and cadmium were linked to human infection and a clonal lineage originating in animals (P, 0.05). Altogether, S. saprophyticus was highly resistant to heavy metals and accumulated multiple metal resistance determinants. The highest arsenic and cadmium resistance levels were associated with infection, suggesting resistance to these metals is relevant for S. saprophyticus pathogenicityAmerican Society for MicrobiologyRepositório da Universidade de LisboaLawal, Opeyemi U.Fraqueza, M. J.Worning, PederBouchami, Ons2022-02-06T22:43:12Z2021-06-172021-06-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23413engLawal OU, Fraqueza MJ, Worning P, Bouchami O, Bartels MD, Goncalves L, Paixão P, Goncalves E, Toscano C, Empel J, Urbans M, Domínguez MA, Westh H, de Lencastre H, Miragaia M. 2021. Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals. Antimicrobial Agents Chemotherapy, 65(7):e02685-20. Doi: 10.1128/AAC.02685-200066-480410.1128/AAC.02685-201098-6596info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:58Zoai:www.repository.utl.pt:10400.5/23413Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:39.181473Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals |
title |
Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals |
spellingShingle |
Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals Lawal, Opeyemi U. Staphylococcus saprophyticus Urinary tract infection Environment Heavy metals Whole-genome sequencing Pan-GWAS Metal resistance determinants Copper Zinc Arsenic Cadmium Metal resistance |
title_short |
Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals |
title_full |
Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals |
title_fullStr |
Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals |
title_full_unstemmed |
Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals |
title_sort |
Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals |
author |
Lawal, Opeyemi U. |
author_facet |
Lawal, Opeyemi U. Fraqueza, M. J. Worning, Peder Bouchami, Ons |
author_role |
author |
author2 |
Fraqueza, M. J. Worning, Peder Bouchami, Ons |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Lawal, Opeyemi U. Fraqueza, M. J. Worning, Peder Bouchami, Ons |
dc.subject.por.fl_str_mv |
Staphylococcus saprophyticus Urinary tract infection Environment Heavy metals Whole-genome sequencing Pan-GWAS Metal resistance determinants Copper Zinc Arsenic Cadmium Metal resistance |
topic |
Staphylococcus saprophyticus Urinary tract infection Environment Heavy metals Whole-genome sequencing Pan-GWAS Metal resistance determinants Copper Zinc Arsenic Cadmium Metal resistance |
description |
Research Areas: Microbiology ; Pharmacology & Pharmacy |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-17 2021-06-17T00:00:00Z 2022-02-06T22:43:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/23413 |
url |
http://hdl.handle.net/10400.5/23413 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lawal OU, Fraqueza MJ, Worning P, Bouchami O, Bartels MD, Goncalves L, Paixão P, Goncalves E, Toscano C, Empel J, Urbans M, Domínguez MA, Westh H, de Lencastre H, Miragaia M. 2021. Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals. Antimicrobial Agents Chemotherapy, 65(7):e02685-20. Doi: 10.1128/AAC.02685-20 0066-4804 10.1128/AAC.02685-20 1098-6596 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131170987835392 |