Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/28800 |
Resumo: | Chestnut blight, caused by Cryphonectria parasitica, is a severe disease that may be biologically controlled by the use of hypovirulent strains, but the diversity of the pathogen population affects biocontrol feasibility. Villuercas–Ibores–Jara, the Jerte Valley and La Vera are the main chestnut production districts in Cáceres (central-western Spain). The purpose of this study is to determine the Vegetative Compatibility Groups (VCGs) and mating types in these districts and to identify hypovirulent isolates to be used in biological control. The VCGs were determined by the merging/ barrage response; PCR tests were used for the mating type determination and CHV-1 hypovirus detection. In total, 40 sites were surveyed and 269 isolates were obtained, most of them (227) from the Villuercas–Ibores–Jara district, where EU11 was the predominant VCG (88.1%) with EU1 (6.6%) and EU12 (4.4%) being also present. In the Jerte Valley and La Vera, EU1 (61.9%), EU11 (11.9%) and EU12 (11.9%) were the predominant VCGs. Both mating types were detected (48% MAT-1; 50% MAT-2) and in general, only one mating type was found in each site. The presence of Cryphonectria hypovirus 1 (CHV-1), subtype I, was identified in only one isolate (VCG EU11) from Villuercas–Ibores–Jara. The characteristics of the C. parasitica population in this district and the occurrence of CHV-1 hypovirus support the potential of successful biological control in Villuercas–Ibores–Jara using hypovirulent strains, while in the Jerte Valley and La Vera only preventive measures are recommended. |
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Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strainsCryphonectria hypovirusHypovirulenceMating typesPreventive measuresVegetative compatibility groupsChestnut blight, caused by Cryphonectria parasitica, is a severe disease that may be biologically controlled by the use of hypovirulent strains, but the diversity of the pathogen population affects biocontrol feasibility. Villuercas–Ibores–Jara, the Jerte Valley and La Vera are the main chestnut production districts in Cáceres (central-western Spain). The purpose of this study is to determine the Vegetative Compatibility Groups (VCGs) and mating types in these districts and to identify hypovirulent isolates to be used in biological control. The VCGs were determined by the merging/ barrage response; PCR tests were used for the mating type determination and CHV-1 hypovirus detection. In total, 40 sites were surveyed and 269 isolates were obtained, most of them (227) from the Villuercas–Ibores–Jara district, where EU11 was the predominant VCG (88.1%) with EU1 (6.6%) and EU12 (4.4%) being also present. In the Jerte Valley and La Vera, EU1 (61.9%), EU11 (11.9%) and EU12 (11.9%) were the predominant VCGs. Both mating types were detected (48% MAT-1; 50% MAT-2) and in general, only one mating type was found in each site. The presence of Cryphonectria hypovirus 1 (CHV-1), subtype I, was identified in only one isolate (VCG EU11) from Villuercas–Ibores–Jara. The characteristics of the C. parasitica population in this district and the occurrence of CHV-1 hypovirus support the potential of successful biological control in Villuercas–Ibores–Jara using hypovirulent strains, while in the Jerte Valley and La Vera only preventive measures are recommended.This research was supported by the Junta de Extremadura and co-funded by the European Regional Development Fund (ERDF) (AGA001 (GR18196); MIPEX and CCESAGROS projects) and the European Agricultural Fund for Rural Development (EAFRD) (Operative Group CASTANEA (PGOF/35/2019)). M.B. García-García was the recipient of a PhD grant from INIA (PRE2019-087630).MDPIBiblioteca Digital do IPBRodríguez-Molina, María del CarmenGarcía-García, María BlancaOsuna, María DoloresGouveia, Maria EugéniaSerrano-Pérez, Paula2023-10-20T10:33:33Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/28800engRodríguez-Molina, María del Carmen; García-García, María Blanca; Osuna, María Dolores; Gouveia, Eugénia; Serrano-Pérez, Paula (2023). Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains. Agronomy. eISSN 2073-4395. 13:5, p. 1-1310.3390/agronomy130512082073-4395info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T11:02:52Zoai:bibliotecadigital.ipb.pt:10198/28800Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:18:45.639963Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains |
title |
Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains |
spellingShingle |
Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains Rodríguez-Molina, María del Carmen Cryphonectria hypovirus Hypovirulence Mating types Preventive measures Vegetative compatibility groups |
title_short |
Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains |
title_full |
Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains |
title_fullStr |
Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains |
title_full_unstemmed |
Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains |
title_sort |
Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains |
author |
Rodríguez-Molina, María del Carmen |
author_facet |
Rodríguez-Molina, María del Carmen García-García, María Blanca Osuna, María Dolores Gouveia, Maria Eugénia Serrano-Pérez, Paula |
author_role |
author |
author2 |
García-García, María Blanca Osuna, María Dolores Gouveia, Maria Eugénia Serrano-Pérez, Paula |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Rodríguez-Molina, María del Carmen García-García, María Blanca Osuna, María Dolores Gouveia, Maria Eugénia Serrano-Pérez, Paula |
dc.subject.por.fl_str_mv |
Cryphonectria hypovirus Hypovirulence Mating types Preventive measures Vegetative compatibility groups |
topic |
Cryphonectria hypovirus Hypovirulence Mating types Preventive measures Vegetative compatibility groups |
description |
Chestnut blight, caused by Cryphonectria parasitica, is a severe disease that may be biologically controlled by the use of hypovirulent strains, but the diversity of the pathogen population affects biocontrol feasibility. Villuercas–Ibores–Jara, the Jerte Valley and La Vera are the main chestnut production districts in Cáceres (central-western Spain). The purpose of this study is to determine the Vegetative Compatibility Groups (VCGs) and mating types in these districts and to identify hypovirulent isolates to be used in biological control. The VCGs were determined by the merging/ barrage response; PCR tests were used for the mating type determination and CHV-1 hypovirus detection. In total, 40 sites were surveyed and 269 isolates were obtained, most of them (227) from the Villuercas–Ibores–Jara district, where EU11 was the predominant VCG (88.1%) with EU1 (6.6%) and EU12 (4.4%) being also present. In the Jerte Valley and La Vera, EU1 (61.9%), EU11 (11.9%) and EU12 (11.9%) were the predominant VCGs. Both mating types were detected (48% MAT-1; 50% MAT-2) and in general, only one mating type was found in each site. The presence of Cryphonectria hypovirus 1 (CHV-1), subtype I, was identified in only one isolate (VCG EU11) from Villuercas–Ibores–Jara. The characteristics of the C. parasitica population in this district and the occurrence of CHV-1 hypovirus support the potential of successful biological control in Villuercas–Ibores–Jara using hypovirulent strains, while in the Jerte Valley and La Vera only preventive measures are recommended. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-20T10:33:33Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/28800 |
url |
http://hdl.handle.net/10198/28800 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rodríguez-Molina, María del Carmen; García-García, María Blanca; Osuna, María Dolores; Gouveia, Eugénia; Serrano-Pérez, Paula (2023). Various population structures of Cryphonectria parasitica in Cáceres (Spain) determine the feasibility of the biological control of chestnut blight with hypovirulent strains. Agronomy. eISSN 2073-4395. 13:5, p. 1-13 10.3390/agronomy13051208 2073-4395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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MDPI |
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MDPI |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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