Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus

Detalhes bibliográficos
Autor(a) principal: Ouni, Omar Abdelaziz
Data de Publicação: 2020
Outros Autores: Jorge, Lurdes, Moura, Luísa, Coelho, Valentim, Gouveia, Eugénia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.19/6619
Resumo: Introduction: Cryphonectria parasitica, the causal agent of chestnut blight, causes necrotic lesions (so-called cankers) on the bark of stems and branches of susceptible host trees. Cryphonectria hypovirus 1 (CHV1) infects C. parasitica and reduces the fungus virulence (hypovirulence) and alters the fungus morphology in culture (pigmentation and sporulation capacity). By these characteristics, the strains with hypovirus CHV1 are used in Europe as a biological control agent of chestnut blight. Objectives: The aim of this work is to understand the effect of hypovirus on fungi virulence by comparing the production of some lignin-degrading enzymes and the metabolic profiles of some isogenic virulent and hypovirulent (converted and original) strains. Methods: The virulence of each strain was evaluated by mycelial inoculation on apple fruits (cv. Golden Delicious) and on detached, one year old, chestnut branches. To detect the activity of ligninolytic enzymes (laccases, peroxidases and cellulases), various substrates and indicator compounds were used. The metabolic profile of C. parasitica was evaluated by the Biolog FF system using 95 different carbon sources. Results: Virulent strains were found to cause more significant necrotic lesions in chestnut branches (p<0.05) and to produce larger lignin-degrading enzymes. The use of Biolog FF MicroPlates indicated that the use of 95 carbon sources five isolates of C. parasitica were significantly different (p <0.001), when the substrates were grouped into six types of chemical compounds. The highest AWCD values were obtained for carbohydrates, carboxylic acids and polymers, and the lowest values for amines/amides, amino acids and miscellaneous. Conclusions: Virulence evaluation of C. parasitica strains is important to study the hypovirulence processes mediated by the hypovirus CHV1. The detached branches of chestnut were, in our study, more suitable than apple fruits test in discriminating hypovirulent from virulent strains of C. parasitica. Virulent strains showed higher activity of acid-tannic inducible laccase (Lac3) and other lignin-degrading enzymes (LiP, MnP, and cellulase) when compared with hypovirulent ones. The results of the metabolic profiles studies may lead to new perspectives for understanding the biological process used by the hypovirus therefore, this may suggest a method for discriminating hypovirulent strains and study ecology and field fitness in this fungal strains.
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spelling Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirusCryphonectria parasiticavirulencemetabolic profileBiolog FF MicroPlatesligninolytic enzymesCryphonectria hypovirus 1 (CHV1)Introduction: Cryphonectria parasitica, the causal agent of chestnut blight, causes necrotic lesions (so-called cankers) on the bark of stems and branches of susceptible host trees. Cryphonectria hypovirus 1 (CHV1) infects C. parasitica and reduces the fungus virulence (hypovirulence) and alters the fungus morphology in culture (pigmentation and sporulation capacity). By these characteristics, the strains with hypovirus CHV1 are used in Europe as a biological control agent of chestnut blight. Objectives: The aim of this work is to understand the effect of hypovirus on fungi virulence by comparing the production of some lignin-degrading enzymes and the metabolic profiles of some isogenic virulent and hypovirulent (converted and original) strains. Methods: The virulence of each strain was evaluated by mycelial inoculation on apple fruits (cv. Golden Delicious) and on detached, one year old, chestnut branches. To detect the activity of ligninolytic enzymes (laccases, peroxidases and cellulases), various substrates and indicator compounds were used. The metabolic profile of C. parasitica was evaluated by the Biolog FF system using 95 different carbon sources. Results: Virulent strains were found to cause more significant necrotic lesions in chestnut branches (p<0.05) and to produce larger lignin-degrading enzymes. The use of Biolog FF MicroPlates indicated that the use of 95 carbon sources five isolates of C. parasitica were significantly different (p <0.001), when the substrates were grouped into six types of chemical compounds. The highest AWCD values were obtained for carbohydrates, carboxylic acids and polymers, and the lowest values for amines/amides, amino acids and miscellaneous. Conclusions: Virulence evaluation of C. parasitica strains is important to study the hypovirulence processes mediated by the hypovirus CHV1. The detached branches of chestnut were, in our study, more suitable than apple fruits test in discriminating hypovirulent from virulent strains of C. parasitica. Virulent strains showed higher activity of acid-tannic inducible laccase (Lac3) and other lignin-degrading enzymes (LiP, MnP, and cellulase) when compared with hypovirulent ones. The results of the metabolic profiles studies may lead to new perspectives for understanding the biological process used by the hypovirus therefore, this may suggest a method for discriminating hypovirulent strains and study ecology and field fitness in this fungal strains.Instituto Politécnico de ViseuRepositório Científico do Instituto Politécnico de ViseuOuni, Omar AbdelazizJorge, LurdesMoura, LuísaCoelho, ValentimGouveia, Eugénia2021-02-23T17:18:23Z20202021-02-23T16:44:15Z2020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.19/6619engOuni, O. A., Jorge, L., Moura, L., Coelho, V., & Gouveia, E. (2020). Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains converted by CHV1 hypovirus. Millenium, 2(13), 11-21. DOI: 10.29352/mill0213.01.0028510.29352/mill0213.01.00285info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-01-16T15:28:51Zoai:repositorio.ipv.pt:10400.19/6619Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:44:27.311166Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus
title Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus
spellingShingle Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus
Ouni, Omar Abdelaziz
Cryphonectria parasitica
virulence
metabolic profile
Biolog FF MicroPlates
ligninolytic enzymes
Cryphonectria hypovirus 1 (CHV1)
title_short Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus
title_full Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus
title_fullStr Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus
title_full_unstemmed Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus
title_sort Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus
author Ouni, Omar Abdelaziz
author_facet Ouni, Omar Abdelaziz
Jorge, Lurdes
Moura, Luísa
Coelho, Valentim
Gouveia, Eugénia
author_role author
author2 Jorge, Lurdes
Moura, Luísa
Coelho, Valentim
Gouveia, Eugénia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico de Viseu
dc.contributor.author.fl_str_mv Ouni, Omar Abdelaziz
Jorge, Lurdes
Moura, Luísa
Coelho, Valentim
Gouveia, Eugénia
dc.subject.por.fl_str_mv Cryphonectria parasitica
virulence
metabolic profile
Biolog FF MicroPlates
ligninolytic enzymes
Cryphonectria hypovirus 1 (CHV1)
topic Cryphonectria parasitica
virulence
metabolic profile
Biolog FF MicroPlates
ligninolytic enzymes
Cryphonectria hypovirus 1 (CHV1)
description Introduction: Cryphonectria parasitica, the causal agent of chestnut blight, causes necrotic lesions (so-called cankers) on the bark of stems and branches of susceptible host trees. Cryphonectria hypovirus 1 (CHV1) infects C. parasitica and reduces the fungus virulence (hypovirulence) and alters the fungus morphology in culture (pigmentation and sporulation capacity). By these characteristics, the strains with hypovirus CHV1 are used in Europe as a biological control agent of chestnut blight. Objectives: The aim of this work is to understand the effect of hypovirus on fungi virulence by comparing the production of some lignin-degrading enzymes and the metabolic profiles of some isogenic virulent and hypovirulent (converted and original) strains. Methods: The virulence of each strain was evaluated by mycelial inoculation on apple fruits (cv. Golden Delicious) and on detached, one year old, chestnut branches. To detect the activity of ligninolytic enzymes (laccases, peroxidases and cellulases), various substrates and indicator compounds were used. The metabolic profile of C. parasitica was evaluated by the Biolog FF system using 95 different carbon sources. Results: Virulent strains were found to cause more significant necrotic lesions in chestnut branches (p<0.05) and to produce larger lignin-degrading enzymes. The use of Biolog FF MicroPlates indicated that the use of 95 carbon sources five isolates of C. parasitica were significantly different (p <0.001), when the substrates were grouped into six types of chemical compounds. The highest AWCD values were obtained for carbohydrates, carboxylic acids and polymers, and the lowest values for amines/amides, amino acids and miscellaneous. Conclusions: Virulence evaluation of C. parasitica strains is important to study the hypovirulence processes mediated by the hypovirus CHV1. The detached branches of chestnut were, in our study, more suitable than apple fruits test in discriminating hypovirulent from virulent strains of C. parasitica. Virulent strains showed higher activity of acid-tannic inducible laccase (Lac3) and other lignin-degrading enzymes (LiP, MnP, and cellulase) when compared with hypovirulent ones. The results of the metabolic profiles studies may lead to new perspectives for understanding the biological process used by the hypovirus therefore, this may suggest a method for discriminating hypovirulent strains and study ecology and field fitness in this fungal strains.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-02-23T17:18:23Z
2021-02-23T16:44:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.19/6619
url http://hdl.handle.net/10400.19/6619
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ouni, O. A., Jorge, L., Moura, L., Coelho, V., & Gouveia, E. (2020). Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains converted by CHV1 hypovirus. Millenium, 2(13), 11-21. DOI: 10.29352/mill0213.01.00285
10.29352/mill0213.01.00285
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Politécnico de Viseu
publisher.none.fl_str_mv Instituto Politécnico de Viseu
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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