The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/58826 |
Resumo: | Background: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased ( almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease ( approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression ( 2-fold) in the hippocampus that was delayed until 1 day after injury.Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury. |
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The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injuryAnimalsBase SequenceBrain InjuriesChoroid PlexusGene Expression ProfilingHippocampusHumansImmunohistochemistryIn Situ HybridizationInflammationKeratinsLipopolysaccharidesMaleMembrane GlycoproteinsMembrane ProteinsMicePlatelet Glycoprotein GPIb-IX ComplexRNA, MessengerRatsReceptors, G-Protein-CoupledTime FactorsUp-RegulationScience & TechnologyBackground: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased ( almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease ( approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression ( 2-fold) in the hippocampus that was delayed until 1 day after injury.Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury.The authors wish to thank Anders Hellström and Julie Sarri for technical assistance. ML was supported by Hjärnfonden (Brain Research Foundation). Studies were supported by grants from the Swedish Research Foundation and Novo Nordisk to HBS and TBI studies were supported by grant NS-49479 from the NIH to AC. JAP studies were supported by grant POCTI/SAU-NEU/56618/2004 from FEDER/Portuguese Foundation for Science and Technology (FCT). FM has a PhD fellowship from FCT.info:eu-repo/semantics/publishedVersionBioMed Central (BMC)Universidade do MinhoPickering, ChrisHägglund, MariaSzmydynger-Chodobska, JoannaMarques, FernandaPalha, Joana AlmeidaWaller, LinnChodobski, AdamFredriksson, RobertLagerström, Malin C.Schiöth, Helgi B.20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/58826eng1471-220210.1186/1471-2202-9-9718834514info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:56Zoai:repositorium.sdum.uminho.pt:1822/58826Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:23:50.243101Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury |
title |
The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury |
spellingShingle |
The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury Pickering, Chris Animals Base Sequence Brain Injuries Choroid Plexus Gene Expression Profiling Hippocampus Humans Immunohistochemistry In Situ Hybridization Inflammation Keratins Lipopolysaccharides Male Membrane Glycoproteins Membrane Proteins Mice Platelet Glycoprotein GPIb-IX Complex RNA, Messenger Rats Receptors, G-Protein-Coupled Time Factors Up-Regulation Science & Technology |
title_short |
The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury |
title_full |
The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury |
title_fullStr |
The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury |
title_full_unstemmed |
The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury |
title_sort |
The Adhesion GPCR GPR125 is specifically expressed in the choroid plexus and is upregulated following brain injury |
author |
Pickering, Chris |
author_facet |
Pickering, Chris Hägglund, Maria Szmydynger-Chodobska, Joanna Marques, Fernanda Palha, Joana Almeida Waller, Linn Chodobski, Adam Fredriksson, Robert Lagerström, Malin C. Schiöth, Helgi B. |
author_role |
author |
author2 |
Hägglund, Maria Szmydynger-Chodobska, Joanna Marques, Fernanda Palha, Joana Almeida Waller, Linn Chodobski, Adam Fredriksson, Robert Lagerström, Malin C. Schiöth, Helgi B. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pickering, Chris Hägglund, Maria Szmydynger-Chodobska, Joanna Marques, Fernanda Palha, Joana Almeida Waller, Linn Chodobski, Adam Fredriksson, Robert Lagerström, Malin C. Schiöth, Helgi B. |
dc.subject.por.fl_str_mv |
Animals Base Sequence Brain Injuries Choroid Plexus Gene Expression Profiling Hippocampus Humans Immunohistochemistry In Situ Hybridization Inflammation Keratins Lipopolysaccharides Male Membrane Glycoproteins Membrane Proteins Mice Platelet Glycoprotein GPIb-IX Complex RNA, Messenger Rats Receptors, G-Protein-Coupled Time Factors Up-Regulation Science & Technology |
topic |
Animals Base Sequence Brain Injuries Choroid Plexus Gene Expression Profiling Hippocampus Humans Immunohistochemistry In Situ Hybridization Inflammation Keratins Lipopolysaccharides Male Membrane Glycoproteins Membrane Proteins Mice Platelet Glycoprotein GPIb-IX Complex RNA, Messenger Rats Receptors, G-Protein-Coupled Time Factors Up-Regulation Science & Technology |
description |
Background: GPR125 belongs to the family of Adhesion G protein-coupled receptors (GPCRs). A single copy of GPR125 was found in many vertebrate genomes. We also identified a Drosophila sequence, DmCG15744, which shares a common ancestor with the entire Group III of Adhesion GPCRs, and also contains Ig, LRR and HBD domains which were observed in mammalian GPR125.Results: We found specific expression of GPR125 in cells of the choroid plexus using in situ hybridization and protein-specific antibodies and combined in situ/immunohistochemistry co-localization using cytokeratin, a marker specific for epithelial cells. Induction of inflammation by LPS did not change GPR125 expression. However, GPR125 expression was transiently increased ( almost 2-fold) at 4 h after traumatic brain injury (TBI) followed by a decrease ( approximately 4-fold) from 2 days onwards in the choroid plexus as well as increased expression ( 2-fold) in the hippocampus that was delayed until 1 day after injury.Conclusion: These findings suggest that GPR125 plays a functional role in choroidal and hippocampal response to injury. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/58826 |
url |
http://hdl.handle.net/1822/58826 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1471-2202 10.1186/1471-2202-9-97 18834514 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central (BMC) |
publisher.none.fl_str_mv |
BioMed Central (BMC) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132715049549824 |