Transcriptome signature of the adult mouse choroid plexus
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/16938 |
Resumo: | Background: Although the gene expression profile of several tissues in humans and in rodent animal models has been explored, analysis of the complete choroid plexus (CP) transcriptome is still lacking. A better characterization of the CP transcriptome can provide key insights into its functions as one of the barriers that separate the brain from the periphery and in the production of cerebrospinal fluid. Methods: This work extends further what is known about the mouse CP transcriptome through a microarray analysis of CP tissue from normal mice under physiological conditions. Results: We found that the genes most highly expressed are those implicated in energy metabolism (oxidative phosphorylation, glycolysis/gluconeogenesis) and in ribosomal function, which is in agreement with the secretory nature of the CP. On the other hand, genes encoding for immune mediators are among those with lower expression in basal conditions. In addition, we found genes known to be relevant during brain development, and not previously identified to be expressed in the CP, including those encoding for various axonal guidance and angiogenesis molecules and for growth factors. Some of these are known to influence the neural stem cell niche in the subventricular zone, highlighting the involvement of the CP as a likely modulator of neurogenesis. Interestingly, our observations confirm that the CP transcriptome is unique, displaying low homology with that of other tissues. Of note, we describe here that the closest similarity is with the transcriptome of the endothelial cells of the blood-brain barrier. Conclusions: Based on the data presented here, it will now be possible to further explore the function of particular proteins of the CP secretome in health and in disease. |
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Transcriptome signature of the adult mouse choroid plexusChoroid plexusBasal transcriptomeMiceScience & TechnologyBackground: Although the gene expression profile of several tissues in humans and in rodent animal models has been explored, analysis of the complete choroid plexus (CP) transcriptome is still lacking. A better characterization of the CP transcriptome can provide key insights into its functions as one of the barriers that separate the brain from the periphery and in the production of cerebrospinal fluid. Methods: This work extends further what is known about the mouse CP transcriptome through a microarray analysis of CP tissue from normal mice under physiological conditions. Results: We found that the genes most highly expressed are those implicated in energy metabolism (oxidative phosphorylation, glycolysis/gluconeogenesis) and in ribosomal function, which is in agreement with the secretory nature of the CP. On the other hand, genes encoding for immune mediators are among those with lower expression in basal conditions. In addition, we found genes known to be relevant during brain development, and not previously identified to be expressed in the CP, including those encoding for various axonal guidance and angiogenesis molecules and for growth factors. Some of these are known to influence the neural stem cell niche in the subventricular zone, highlighting the involvement of the CP as a likely modulator of neurogenesis. Interestingly, our observations confirm that the CP transcriptome is unique, displaying low homology with that of other tissues. Of note, we describe here that the closest similarity is with the transcriptome of the endothelial cells of the blood-brain barrier. Conclusions: Based on the data presented here, it will now be possible to further explore the function of particular proteins of the CP secretome in health and in disease.Grant from The Dana Foundation (USA); Marques F is recipient of postdoctoral fellowship from Fundação para a Ciência e Tecnologia (Portugal)BioMed Central (BMC)Universidade do MinhoMarques, Fernanda Cristina Gomes de SousaSousa, João CarlosCoppola, GiovanniGao, FuyingPuga, RenatoBrentani, HelenaGeschwind, Daniel H.Sousa, NunoNeves, Margarida CorreiaPalha, Joana Almeida2011-012011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/16938eng2045-811810.1186/2045-8118-8-10http://www.fluidsbarrierscns.com/content/8/1/10info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:57:32Zoai:repositorium.sdum.uminho.pt:1822/16938Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:47:11.558639Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Transcriptome signature of the adult mouse choroid plexus |
title |
Transcriptome signature of the adult mouse choroid plexus |
spellingShingle |
Transcriptome signature of the adult mouse choroid plexus Marques, Fernanda Cristina Gomes de Sousa Choroid plexus Basal transcriptome Mice Science & Technology |
title_short |
Transcriptome signature of the adult mouse choroid plexus |
title_full |
Transcriptome signature of the adult mouse choroid plexus |
title_fullStr |
Transcriptome signature of the adult mouse choroid plexus |
title_full_unstemmed |
Transcriptome signature of the adult mouse choroid plexus |
title_sort |
Transcriptome signature of the adult mouse choroid plexus |
author |
Marques, Fernanda Cristina Gomes de Sousa |
author_facet |
Marques, Fernanda Cristina Gomes de Sousa Sousa, João Carlos Coppola, Giovanni Gao, Fuying Puga, Renato Brentani, Helena Geschwind, Daniel H. Sousa, Nuno Neves, Margarida Correia Palha, Joana Almeida |
author_role |
author |
author2 |
Sousa, João Carlos Coppola, Giovanni Gao, Fuying Puga, Renato Brentani, Helena Geschwind, Daniel H. Sousa, Nuno Neves, Margarida Correia Palha, Joana Almeida |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Marques, Fernanda Cristina Gomes de Sousa Sousa, João Carlos Coppola, Giovanni Gao, Fuying Puga, Renato Brentani, Helena Geschwind, Daniel H. Sousa, Nuno Neves, Margarida Correia Palha, Joana Almeida |
dc.subject.por.fl_str_mv |
Choroid plexus Basal transcriptome Mice Science & Technology |
topic |
Choroid plexus Basal transcriptome Mice Science & Technology |
description |
Background: Although the gene expression profile of several tissues in humans and in rodent animal models has been explored, analysis of the complete choroid plexus (CP) transcriptome is still lacking. A better characterization of the CP transcriptome can provide key insights into its functions as one of the barriers that separate the brain from the periphery and in the production of cerebrospinal fluid. Methods: This work extends further what is known about the mouse CP transcriptome through a microarray analysis of CP tissue from normal mice under physiological conditions. Results: We found that the genes most highly expressed are those implicated in energy metabolism (oxidative phosphorylation, glycolysis/gluconeogenesis) and in ribosomal function, which is in agreement with the secretory nature of the CP. On the other hand, genes encoding for immune mediators are among those with lower expression in basal conditions. In addition, we found genes known to be relevant during brain development, and not previously identified to be expressed in the CP, including those encoding for various axonal guidance and angiogenesis molecules and for growth factors. Some of these are known to influence the neural stem cell niche in the subventricular zone, highlighting the involvement of the CP as a likely modulator of neurogenesis. Interestingly, our observations confirm that the CP transcriptome is unique, displaying low homology with that of other tissues. Of note, we describe here that the closest similarity is with the transcriptome of the endothelial cells of the blood-brain barrier. Conclusions: Based on the data presented here, it will now be possible to further explore the function of particular proteins of the CP secretome in health and in disease. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01 2011-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/16938 |
url |
http://hdl.handle.net/1822/16938 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2045-8118 10.1186/2045-8118-8-10 http://www.fluidsbarrierscns.com/content/8/1/10 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central (BMC) |
publisher.none.fl_str_mv |
BioMed Central (BMC) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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