Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/169516 |
Resumo: | Funding Information: This research was funded by the FCT-Foundation for Science and Technology, I.P., through research grants PTDC/CVT-CVT/28908/2017 (http://doi.org/10.54499/PTDC/CVT-CVT/28908/2017) and PTDC/CVT-CVT/0228/2020 (http://doi.org/10.54499/PTDC/CVT-CVT/0228/2020) and by national funds within the scope of Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020), AL4AnimalS—LA/P/0059/2020, Global Health and Tropical Medicine (GHTM, UID/04413/2020), and LA-REAL—LA/P/0117/2020. Publisher Copyright: © 2024 by the authors. |
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Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Responsecytokinesdoginnate immunityL. infantumLeishmania amazonensisLeishmania extracellular vesiclesmajor histocompatibility complexpattern recognition receptorsperipheral blood-derived dendritic cells (moDCs)Biochemistry, Genetics and Molecular Biology(all)Parasitologyveterinary(all)SDG 3 - Good Health and Well-beingFunding Information: This research was funded by the FCT-Foundation for Science and Technology, I.P., through research grants PTDC/CVT-CVT/28908/2017 (http://doi.org/10.54499/PTDC/CVT-CVT/28908/2017) and PTDC/CVT-CVT/0228/2020 (http://doi.org/10.54499/PTDC/CVT-CVT/0228/2020) and by national funds within the scope of Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020), AL4AnimalS—LA/P/0059/2020, Global Health and Tropical Medicine (GHTM, UID/04413/2020), and LA-REAL—LA/P/0117/2020. Publisher Copyright: © 2024 by the authors.Dendritic cells (DCs) capture pathogens and process antigens, playing a crucial role in activating naïve T cells, bridging the gap between innate and acquired immunity. However, little is known about DC activation when facing Leishmania parasites. Thus, this study investigates in vitro activity of canine peripheral blood-derived DCs (moDCs) exposed to L. infantum and L. amazonensis parasites and their extracellular vesicles (EVs). L. infantum increased toll-like receptor 4 gene expression in synergy with nuclear factor κB activation and the generation of pro-inflammatory cytokines. This parasite also induced the expression of class II molecules of major histocompatibility complex (MHC) and upregulated co-stimulatory molecule CD86, which, together with the release of chemokine CXCL16, can attract and help in T lymphocyte activation. In contrast, L. amazonensis induced moDCs to generate a mix of pro- and anti-inflammatory cytokines, indicating that this parasite can establish a different immune relationship with DCs. EVs promoted moDCs to express class I MHC associated with the upregulation of co-stimulatory molecules and the release of CXCL16, suggesting that EVs can modulate moDCs to attract cytotoxic CD8+ T cells. Thus, these parasites and their EVs can shape DC activation. A detailed understanding of DC activation may open new avenues for the development of advanced leishmaniasis control strategies.Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)TB, HIV and opportunistic diseases and pathogens (THOP)RUNValério-Bolas, AnaMeunier, MafaldaPalma-Marques, JoanaRodrigues, ArmandaSantos, Ana MargaridaNunes, TelmoFerreira, RuiArmada, AnaAlves, João CarlosAntunes, WilsonCardoso, InêsMesquita-Gabriel, SofiaLobo, LisAlexandre-Pires, GraçaMarques, LuísPereira da Fonseca, IsabelSantos-Gomes, Gabriela2024-07-10T22:19:12Z2024-032024-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/169516eng2073-4409PURE: 93641904https://doi.org/10.3390/cells13050445info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-07-22T01:38:05Zoai:run.unl.pt:10362/169516Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-07-22T01:38:05Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response |
title |
Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response |
spellingShingle |
Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response Valério-Bolas, Ana cytokines dog innate immunity L. infantum Leishmania amazonensis Leishmania extracellular vesicles major histocompatibility complex pattern recognition receptors peripheral blood-derived dendritic cells (moDCs) Biochemistry, Genetics and Molecular Biology(all) Parasitology veterinary(all) SDG 3 - Good Health and Well-being |
title_short |
Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response |
title_full |
Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response |
title_fullStr |
Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response |
title_full_unstemmed |
Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response |
title_sort |
Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response |
author |
Valério-Bolas, Ana |
author_facet |
Valério-Bolas, Ana Meunier, Mafalda Palma-Marques, Joana Rodrigues, Armanda Santos, Ana Margarida Nunes, Telmo Ferreira, Rui Armada, Ana Alves, João Carlos Antunes, Wilson Cardoso, Inês Mesquita-Gabriel, Sofia Lobo, Lis Alexandre-Pires, Graça Marques, Luís Pereira da Fonseca, Isabel Santos-Gomes, Gabriela |
author_role |
author |
author2 |
Meunier, Mafalda Palma-Marques, Joana Rodrigues, Armanda Santos, Ana Margarida Nunes, Telmo Ferreira, Rui Armada, Ana Alves, João Carlos Antunes, Wilson Cardoso, Inês Mesquita-Gabriel, Sofia Lobo, Lis Alexandre-Pires, Graça Marques, Luís Pereira da Fonseca, Isabel Santos-Gomes, Gabriela |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Global Health and Tropical Medicine (GHTM) Instituto de Higiene e Medicina Tropical (IHMT) Vector borne diseases and pathogens (VBD) TB, HIV and opportunistic diseases and pathogens (THOP) RUN |
dc.contributor.author.fl_str_mv |
Valério-Bolas, Ana Meunier, Mafalda Palma-Marques, Joana Rodrigues, Armanda Santos, Ana Margarida Nunes, Telmo Ferreira, Rui Armada, Ana Alves, João Carlos Antunes, Wilson Cardoso, Inês Mesquita-Gabriel, Sofia Lobo, Lis Alexandre-Pires, Graça Marques, Luís Pereira da Fonseca, Isabel Santos-Gomes, Gabriela |
dc.subject.por.fl_str_mv |
cytokines dog innate immunity L. infantum Leishmania amazonensis Leishmania extracellular vesicles major histocompatibility complex pattern recognition receptors peripheral blood-derived dendritic cells (moDCs) Biochemistry, Genetics and Molecular Biology(all) Parasitology veterinary(all) SDG 3 - Good Health and Well-being |
topic |
cytokines dog innate immunity L. infantum Leishmania amazonensis Leishmania extracellular vesicles major histocompatibility complex pattern recognition receptors peripheral blood-derived dendritic cells (moDCs) Biochemistry, Genetics and Molecular Biology(all) Parasitology veterinary(all) SDG 3 - Good Health and Well-being |
description |
Funding Information: This research was funded by the FCT-Foundation for Science and Technology, I.P., through research grants PTDC/CVT-CVT/28908/2017 (http://doi.org/10.54499/PTDC/CVT-CVT/28908/2017) and PTDC/CVT-CVT/0228/2020 (http://doi.org/10.54499/PTDC/CVT-CVT/0228/2020) and by national funds within the scope of Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020), AL4AnimalS—LA/P/0059/2020, Global Health and Tropical Medicine (GHTM, UID/04413/2020), and LA-REAL—LA/P/0117/2020. Publisher Copyright: © 2024 by the authors. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-07-10T22:19:12Z 2024-03 2024-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/169516 |
url |
http://hdl.handle.net/10362/169516 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2073-4409 PURE: 93641904 https://doi.org/10.3390/cells13050445 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817546601642590208 |