Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response

Detalhes bibliográficos
Autor(a) principal: Valério-Bolas, Ana
Data de Publicação: 2024
Outros Autores: Meunier, Mafalda, Palma-Marques, Joana, Rodrigues, Armanda, Santos, Ana Margarida, Nunes, Telmo, Ferreira, Rui, Armada, Ana, Alves, João Carlos, Antunes, Wilson, Cardoso, Inês, Mesquita-Gabriel, Sofia, Lobo, Lis, Alexandre-Pires, Graça, Marques, Luís, Pereira da Fonseca, Isabel, Santos-Gomes, Gabriela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/169516
Resumo: Funding Information: This research was funded by the FCT-Foundation for Science and Technology, I.P., through research grants PTDC/CVT-CVT/28908/2017 (http://doi.org/10.54499/PTDC/CVT-CVT/28908/2017) and PTDC/CVT-CVT/0228/2020 (http://doi.org/10.54499/PTDC/CVT-CVT/0228/2020) and by national funds within the scope of Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020), AL4AnimalS—LA/P/0059/2020, Global Health and Tropical Medicine (GHTM, UID/04413/2020), and LA-REAL—LA/P/0117/2020. Publisher Copyright: © 2024 by the authors.
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spelling Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Responsecytokinesdoginnate immunityL. infantumLeishmania amazonensisLeishmania extracellular vesiclesmajor histocompatibility complexpattern recognition receptorsperipheral blood-derived dendritic cells (moDCs)Biochemistry, Genetics and Molecular Biology(all)Parasitologyveterinary(all)SDG 3 - Good Health and Well-beingFunding Information: This research was funded by the FCT-Foundation for Science and Technology, I.P., through research grants PTDC/CVT-CVT/28908/2017 (http://doi.org/10.54499/PTDC/CVT-CVT/28908/2017) and PTDC/CVT-CVT/0228/2020 (http://doi.org/10.54499/PTDC/CVT-CVT/0228/2020) and by national funds within the scope of Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020), AL4AnimalS—LA/P/0059/2020, Global Health and Tropical Medicine (GHTM, UID/04413/2020), and LA-REAL—LA/P/0117/2020. Publisher Copyright: © 2024 by the authors.Dendritic cells (DCs) capture pathogens and process antigens, playing a crucial role in activating naïve T cells, bridging the gap between innate and acquired immunity. However, little is known about DC activation when facing Leishmania parasites. Thus, this study investigates in vitro activity of canine peripheral blood-derived DCs (moDCs) exposed to L. infantum and L. amazonensis parasites and their extracellular vesicles (EVs). L. infantum increased toll-like receptor 4 gene expression in synergy with nuclear factor κB activation and the generation of pro-inflammatory cytokines. This parasite also induced the expression of class II molecules of major histocompatibility complex (MHC) and upregulated co-stimulatory molecule CD86, which, together with the release of chemokine CXCL16, can attract and help in T lymphocyte activation. In contrast, L. amazonensis induced moDCs to generate a mix of pro- and anti-inflammatory cytokines, indicating that this parasite can establish a different immune relationship with DCs. EVs promoted moDCs to express class I MHC associated with the upregulation of co-stimulatory molecules and the release of CXCL16, suggesting that EVs can modulate moDCs to attract cytotoxic CD8+ T cells. Thus, these parasites and their EVs can shape DC activation. A detailed understanding of DC activation may open new avenues for the development of advanced leishmaniasis control strategies.Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)TB, HIV and opportunistic diseases and pathogens (THOP)RUNValério-Bolas, AnaMeunier, MafaldaPalma-Marques, JoanaRodrigues, ArmandaSantos, Ana MargaridaNunes, TelmoFerreira, RuiArmada, AnaAlves, João CarlosAntunes, WilsonCardoso, InêsMesquita-Gabriel, SofiaLobo, LisAlexandre-Pires, GraçaMarques, LuísPereira da Fonseca, IsabelSantos-Gomes, Gabriela2024-07-10T22:19:12Z2024-032024-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/169516eng2073-4409PURE: 93641904https://doi.org/10.3390/cells13050445info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-07-22T01:38:05Zoai:run.unl.pt:10362/169516Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-07-22T01:38:05Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response
title Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response
spellingShingle Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response
Valério-Bolas, Ana
cytokines
dog
innate immunity
L. infantum
Leishmania amazonensis
Leishmania extracellular vesicles
major histocompatibility complex
pattern recognition receptors
peripheral blood-derived dendritic cells (moDCs)
Biochemistry, Genetics and Molecular Biology(all)
Parasitology
veterinary(all)
SDG 3 - Good Health and Well-being
title_short Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response
title_full Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response
title_fullStr Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response
title_full_unstemmed Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response
title_sort Exploiting Leishmania—Primed Dendritic Cells as Potential Immunomodulators of Canine Immune Response
author Valério-Bolas, Ana
author_facet Valério-Bolas, Ana
Meunier, Mafalda
Palma-Marques, Joana
Rodrigues, Armanda
Santos, Ana Margarida
Nunes, Telmo
Ferreira, Rui
Armada, Ana
Alves, João Carlos
Antunes, Wilson
Cardoso, Inês
Mesquita-Gabriel, Sofia
Lobo, Lis
Alexandre-Pires, Graça
Marques, Luís
Pereira da Fonseca, Isabel
Santos-Gomes, Gabriela
author_role author
author2 Meunier, Mafalda
Palma-Marques, Joana
Rodrigues, Armanda
Santos, Ana Margarida
Nunes, Telmo
Ferreira, Rui
Armada, Ana
Alves, João Carlos
Antunes, Wilson
Cardoso, Inês
Mesquita-Gabriel, Sofia
Lobo, Lis
Alexandre-Pires, Graça
Marques, Luís
Pereira da Fonseca, Isabel
Santos-Gomes, Gabriela
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
Vector borne diseases and pathogens (VBD)
TB, HIV and opportunistic diseases and pathogens (THOP)
RUN
dc.contributor.author.fl_str_mv Valério-Bolas, Ana
Meunier, Mafalda
Palma-Marques, Joana
Rodrigues, Armanda
Santos, Ana Margarida
Nunes, Telmo
Ferreira, Rui
Armada, Ana
Alves, João Carlos
Antunes, Wilson
Cardoso, Inês
Mesquita-Gabriel, Sofia
Lobo, Lis
Alexandre-Pires, Graça
Marques, Luís
Pereira da Fonseca, Isabel
Santos-Gomes, Gabriela
dc.subject.por.fl_str_mv cytokines
dog
innate immunity
L. infantum
Leishmania amazonensis
Leishmania extracellular vesicles
major histocompatibility complex
pattern recognition receptors
peripheral blood-derived dendritic cells (moDCs)
Biochemistry, Genetics and Molecular Biology(all)
Parasitology
veterinary(all)
SDG 3 - Good Health and Well-being
topic cytokines
dog
innate immunity
L. infantum
Leishmania amazonensis
Leishmania extracellular vesicles
major histocompatibility complex
pattern recognition receptors
peripheral blood-derived dendritic cells (moDCs)
Biochemistry, Genetics and Molecular Biology(all)
Parasitology
veterinary(all)
SDG 3 - Good Health and Well-being
description Funding Information: This research was funded by the FCT-Foundation for Science and Technology, I.P., through research grants PTDC/CVT-CVT/28908/2017 (http://doi.org/10.54499/PTDC/CVT-CVT/28908/2017) and PTDC/CVT-CVT/0228/2020 (http://doi.org/10.54499/PTDC/CVT-CVT/0228/2020) and by national funds within the scope of Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020), AL4AnimalS—LA/P/0059/2020, Global Health and Tropical Medicine (GHTM, UID/04413/2020), and LA-REAL—LA/P/0117/2020. Publisher Copyright: © 2024 by the authors.
publishDate 2024
dc.date.none.fl_str_mv 2024-07-10T22:19:12Z
2024-03
2024-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/169516
url http://hdl.handle.net/10362/169516
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2073-4409
PURE: 93641904
https://doi.org/10.3390/cells13050445
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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