Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform

Detalhes bibliográficos
Autor(a) principal: Barroso, M. Fátima
Data de Publicação: 2016
Outros Autores: Luna, M. Alejandra, Tabares, Juan S. Flores, Delerue-Matos, Cristina, Correa, N. Mariano , Moyano, Fernando, Molina, Patricia G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/9254
Resumo: In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored onto SH–DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs were successfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using envi-ronmentally friendly procedures.
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spelling Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platformCovalently decorated vesiclesGold nanoparticlesImmunosensors designSynthesisIn this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored onto SH–DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs were successfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using envi-ronmentally friendly procedures.Beilstein-InstitutRepositório Científico do Instituto Politécnico do PortoBarroso, M. FátimaLuna, M. AlejandraTabares, Juan S. FloresDelerue-Matos, CristinaCorrea, N. Mariano Moyano, FernandoMolina, Patricia G.2017-01-13T10:50:15Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/9254eng10.3762/bjnano.7.58info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:50:24Zoai:recipp.ipp.pt:10400.22/9254Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:29:50.721363Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform
title Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform
spellingShingle Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform
Barroso, M. Fátima
Covalently decorated vesicles
Gold nanoparticles
Immunosensors design
Synthesis
title_short Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform
title_full Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform
title_fullStr Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform
title_full_unstemmed Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform
title_sort Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform
author Barroso, M. Fátima
author_facet Barroso, M. Fátima
Luna, M. Alejandra
Tabares, Juan S. Flores
Delerue-Matos, Cristina
Correa, N. Mariano 
Moyano, Fernando
Molina, Patricia G.
author_role author
author2 Luna, M. Alejandra
Tabares, Juan S. Flores
Delerue-Matos, Cristina
Correa, N. Mariano 
Moyano, Fernando
Molina, Patricia G.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Barroso, M. Fátima
Luna, M. Alejandra
Tabares, Juan S. Flores
Delerue-Matos, Cristina
Correa, N. Mariano 
Moyano, Fernando
Molina, Patricia G.
dc.subject.por.fl_str_mv Covalently decorated vesicles
Gold nanoparticles
Immunosensors design
Synthesis
topic Covalently decorated vesicles
Gold nanoparticles
Immunosensors design
Synthesis
description In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored onto SH–DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs were successfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using envi-ronmentally friendly procedures.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2017-01-13T10:50:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/9254
url http://hdl.handle.net/10400.22/9254
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.3762/bjnano.7.58
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Beilstein-Institut
publisher.none.fl_str_mv Beilstein-Institut
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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