Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/7665 |
Resumo: | Food contaminants are usually studied as isolated molecules, not considering the overall meal components. Notwithstanding, contaminants are not ingested individually, therefore their risks should be assessed in the context of the overall diet. In the present study the influence of three well known dietary patterns, Western (W), Mediterranean (M) and vegetarian (V), on the bioaccessibility and intestinal transport of polycyclic aromatic hydrocarbons (PAHs) (benzo[a]pyrene (BaP) and benzo[b]fluoranthene (BbF)), heterocyclic aromatic amines (HAAs) (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and 2-Amino-9H-pyrido[2,3-b]indole (AAC)) and mycotoxins (MY) (aflatoxin B2 (AB2) and ochratoxin A (OTA)) was evaluated. Whole meals representative of W, M and V patterns were spiked with 100 µg kg-1 of each contaminant and subjected to the Infogest in vitro digestion method. Intestinal transport was performed using Caco-2 cells in apical/basolateral inserts. Contaminants were quantified by QuEChERS/HPLC/Fluorescence analysis. The dietary pattern itself influenced significantly the bioaccessibility of some contaminants, since higher bioaccessibility of HAAs (PhIP and AAC) was observed for V diet, while higher bioaccessibility of PAHs (BBF and BAP) and the MY (OTA) was observed for W diet. Concerning intestinal transport, the effect of the diet matrices was less noticed. Notwithstanding, AAC transport increased with W diet, while AB2s transport increased with the V diet. Regarding PAHs the three patterns either blocked (BbF) or reduced (BaP) the transport. Besides the well known nutritional, protective or deleterious effects of the different dietary patterns, the increased bioaccessibility or intestinal transport of some food contaminants, can have an additional influence on the global health impact. |
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Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assaysCaco-2 CellsHeterocyclic AminesINFOGEST Digestion MethodIntestinal Transport; Mediterranean DietMycotoxinsPolycyclic Aromatic HydrocarbonsVegetarian DietWestern DietÁgua e SoloFood contaminants are usually studied as isolated molecules, not considering the overall meal components. Notwithstanding, contaminants are not ingested individually, therefore their risks should be assessed in the context of the overall diet. In the present study the influence of three well known dietary patterns, Western (W), Mediterranean (M) and vegetarian (V), on the bioaccessibility and intestinal transport of polycyclic aromatic hydrocarbons (PAHs) (benzo[a]pyrene (BaP) and benzo[b]fluoranthene (BbF)), heterocyclic aromatic amines (HAAs) (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and 2-Amino-9H-pyrido[2,3-b]indole (AAC)) and mycotoxins (MY) (aflatoxin B2 (AB2) and ochratoxin A (OTA)) was evaluated. Whole meals representative of W, M and V patterns were spiked with 100 µg kg-1 of each contaminant and subjected to the Infogest in vitro digestion method. Intestinal transport was performed using Caco-2 cells in apical/basolateral inserts. Contaminants were quantified by QuEChERS/HPLC/Fluorescence analysis. The dietary pattern itself influenced significantly the bioaccessibility of some contaminants, since higher bioaccessibility of HAAs (PhIP and AAC) was observed for V diet, while higher bioaccessibility of PAHs (BBF and BAP) and the MY (OTA) was observed for W diet. Concerning intestinal transport, the effect of the diet matrices was less noticed. Notwithstanding, AAC transport increased with W diet, while AB2s transport increased with the V diet. Regarding PAHs the three patterns either blocked (BbF) or reduced (BaP) the transport. Besides the well known nutritional, protective or deleterious effects of the different dietary patterns, the increased bioaccessibility or intestinal transport of some food contaminants, can have an additional influence on the global health impact.Highlights: Dietary pattern influenced the bioaccessibility of some studied food contaminants; The impact of dietary pattern on intestinal transport was less notorious; Dietary pattern affects contaminants bioaccessibility/absorption and health.M. A. Faria acknowledges Fundação para a Ciência e a Tecnologia and Norte-01-0145-FEDER-0001 the researcher contract. This work was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia in the framework of the project PTDC/SAU-NUT/30322/2017.Elsevier/ Canadian Institute of Food Science and TechnologyRepositório Científico do Instituto Nacional de SaúdeFaria, M.A.Melo, A.Ferreira, Isabel I.M.P.L.V.O.2021-04-08T10:08:25Z2020-112020-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7665engFood Res Int. 2020 Nov;137:109358. doi: 10.1016/j.foodres.2020.109358. Epub 2020 May 28.0963-996910.1016/j.foodres.2020.109358info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:08Zoai:repositorio.insa.pt:10400.18/7665Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:18.653558Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays |
title |
Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays |
spellingShingle |
Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays Faria, M.A. Caco-2 Cells Heterocyclic Amines INFOGEST Digestion Method Intestinal Transport; Mediterranean Diet Mycotoxins Polycyclic Aromatic Hydrocarbons Vegetarian Diet Western Diet Água e Solo |
title_short |
Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays |
title_full |
Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays |
title_fullStr |
Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays |
title_full_unstemmed |
Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays |
title_sort |
Influence of dietary patterns on contaminants bioaccessibility and intestinal transport by in vitro assays |
author |
Faria, M.A. |
author_facet |
Faria, M.A. Melo, A. Ferreira, Isabel I.M.P.L.V.O. |
author_role |
author |
author2 |
Melo, A. Ferreira, Isabel I.M.P.L.V.O. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Faria, M.A. Melo, A. Ferreira, Isabel I.M.P.L.V.O. |
dc.subject.por.fl_str_mv |
Caco-2 Cells Heterocyclic Amines INFOGEST Digestion Method Intestinal Transport; Mediterranean Diet Mycotoxins Polycyclic Aromatic Hydrocarbons Vegetarian Diet Western Diet Água e Solo |
topic |
Caco-2 Cells Heterocyclic Amines INFOGEST Digestion Method Intestinal Transport; Mediterranean Diet Mycotoxins Polycyclic Aromatic Hydrocarbons Vegetarian Diet Western Diet Água e Solo |
description |
Food contaminants are usually studied as isolated molecules, not considering the overall meal components. Notwithstanding, contaminants are not ingested individually, therefore their risks should be assessed in the context of the overall diet. In the present study the influence of three well known dietary patterns, Western (W), Mediterranean (M) and vegetarian (V), on the bioaccessibility and intestinal transport of polycyclic aromatic hydrocarbons (PAHs) (benzo[a]pyrene (BaP) and benzo[b]fluoranthene (BbF)), heterocyclic aromatic amines (HAAs) (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and 2-Amino-9H-pyrido[2,3-b]indole (AAC)) and mycotoxins (MY) (aflatoxin B2 (AB2) and ochratoxin A (OTA)) was evaluated. Whole meals representative of W, M and V patterns were spiked with 100 µg kg-1 of each contaminant and subjected to the Infogest in vitro digestion method. Intestinal transport was performed using Caco-2 cells in apical/basolateral inserts. Contaminants were quantified by QuEChERS/HPLC/Fluorescence analysis. The dietary pattern itself influenced significantly the bioaccessibility of some contaminants, since higher bioaccessibility of HAAs (PhIP and AAC) was observed for V diet, while higher bioaccessibility of PAHs (BBF and BAP) and the MY (OTA) was observed for W diet. Concerning intestinal transport, the effect of the diet matrices was less noticed. Notwithstanding, AAC transport increased with W diet, while AB2s transport increased with the V diet. Regarding PAHs the three patterns either blocked (BbF) or reduced (BaP) the transport. Besides the well known nutritional, protective or deleterious effects of the different dietary patterns, the increased bioaccessibility or intestinal transport of some food contaminants, can have an additional influence on the global health impact. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11 2020-11-01T00:00:00Z 2021-04-08T10:08:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/7665 |
url |
http://hdl.handle.net/10400.18/7665 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Res Int. 2020 Nov;137:109358. doi: 10.1016/j.foodres.2020.109358. Epub 2020 May 28. 0963-9969 10.1016/j.foodres.2020.109358 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier/ Canadian Institute of Food Science and Technology |
publisher.none.fl_str_mv |
Elsevier/ Canadian Institute of Food Science and Technology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132168221360128 |