Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/91391 |
Resumo: | Periplasmic c-type cytochromes are essential for the electron transport between the cytoplasmic membrane bound menquinol oxidase CymA and the terminal ferric iron reductase MtrABC in the outer membrane of Shewanella oneidensis cells. Either STC or FccA are necessary for periplasmic electron transfer. We followed the hypothesis that the elimination of potential competing reactions in the periplasm and the simultaneous overexpression of STC (cctA)could lead to an accelerated electron transfer to the cell surface. The genes nrfA, ccpA, napB and napA were replaced by cctA. This led to a 1.7-fold increased ferric iron reduction rate and a 23% higher current generation in a bioelectrochemical system. Moreover, the quadruple mutant had a higher periplasmic flavin content. Further deletion of fccA and its replacement by cctA resulted in a strain with ferric iron reduction rates similar to the wild type and a lower concentration of periplasmic flavin compared to the quadruple mutant. A transcriptomic analysis revealed that the quadruple mutant had a 3.7-fold higher cctA expression which could not be further increased by the replacement of fccA. This work indicates that a synthetic adaptation of Shewanella towards extracellular respiration holds potential for increased respiratory rates and consequently higher current densities. |
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Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein compositionBioelectrochemical systemCurrent densityElectron transferGenetic engineeringPeriplasmic cytochromeShewanella oneidensisBiophysicsPhysical and Theoretical ChemistryElectrochemistryPeriplasmic c-type cytochromes are essential for the electron transport between the cytoplasmic membrane bound menquinol oxidase CymA and the terminal ferric iron reductase MtrABC in the outer membrane of Shewanella oneidensis cells. Either STC or FccA are necessary for periplasmic electron transfer. We followed the hypothesis that the elimination of potential competing reactions in the periplasm and the simultaneous overexpression of STC (cctA)could lead to an accelerated electron transfer to the cell surface. The genes nrfA, ccpA, napB and napA were replaced by cctA. This led to a 1.7-fold increased ferric iron reduction rate and a 23% higher current generation in a bioelectrochemical system. Moreover, the quadruple mutant had a higher periplasmic flavin content. Further deletion of fccA and its replacement by cctA resulted in a strain with ferric iron reduction rates similar to the wild type and a lower concentration of periplasmic flavin compared to the quadruple mutant. A transcriptomic analysis revealed that the quadruple mutant had a 3.7-fold higher cctA expression which could not be further increased by the replacement of fccA. This work indicates that a synthetic adaptation of Shewanella towards extracellular respiration holds potential for increased respiratory rates and consequently higher current densities.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNDelgado, Veronica PalmaPaquete, Catarina M.Sturm, GunnarGescher, Johannes2024-01-01T01:31:47Z2019-10-012019-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://hdl.handle.net/10362/91391eng1567-5394PURE: 13702085https://doi.org/10.1016/j.bioelechem.2019.04.022info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:40:38Zoai:run.unl.pt:10362/91391Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:37:20.495206Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition |
title |
Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition |
spellingShingle |
Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition Delgado, Veronica Palma Bioelectrochemical system Current density Electron transfer Genetic engineering Periplasmic cytochrome Shewanella oneidensis Biophysics Physical and Theoretical Chemistry Electrochemistry |
title_short |
Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition |
title_full |
Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition |
title_fullStr |
Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition |
title_full_unstemmed |
Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition |
title_sort |
Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition |
author |
Delgado, Veronica Palma |
author_facet |
Delgado, Veronica Palma Paquete, Catarina M. Sturm, Gunnar Gescher, Johannes |
author_role |
author |
author2 |
Paquete, Catarina M. Sturm, Gunnar Gescher, Johannes |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
Delgado, Veronica Palma Paquete, Catarina M. Sturm, Gunnar Gescher, Johannes |
dc.subject.por.fl_str_mv |
Bioelectrochemical system Current density Electron transfer Genetic engineering Periplasmic cytochrome Shewanella oneidensis Biophysics Physical and Theoretical Chemistry Electrochemistry |
topic |
Bioelectrochemical system Current density Electron transfer Genetic engineering Periplasmic cytochrome Shewanella oneidensis Biophysics Physical and Theoretical Chemistry Electrochemistry |
description |
Periplasmic c-type cytochromes are essential for the electron transport between the cytoplasmic membrane bound menquinol oxidase CymA and the terminal ferric iron reductase MtrABC in the outer membrane of Shewanella oneidensis cells. Either STC or FccA are necessary for periplasmic electron transfer. We followed the hypothesis that the elimination of potential competing reactions in the periplasm and the simultaneous overexpression of STC (cctA)could lead to an accelerated electron transfer to the cell surface. The genes nrfA, ccpA, napB and napA were replaced by cctA. This led to a 1.7-fold increased ferric iron reduction rate and a 23% higher current generation in a bioelectrochemical system. Moreover, the quadruple mutant had a higher periplasmic flavin content. Further deletion of fccA and its replacement by cctA resulted in a strain with ferric iron reduction rates similar to the wild type and a lower concentration of periplasmic flavin compared to the quadruple mutant. A transcriptomic analysis revealed that the quadruple mutant had a 3.7-fold higher cctA expression which could not be further increased by the replacement of fccA. This work indicates that a synthetic adaptation of Shewanella towards extracellular respiration holds potential for increased respiratory rates and consequently higher current densities. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-01 2019-10-01T00:00:00Z 2024-01-01T01:31:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/91391 |
url |
http://hdl.handle.net/10362/91391 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1567-5394 PURE: 13702085 https://doi.org/10.1016/j.bioelechem.2019.04.022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137990322159617 |