N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranes

Detalhes bibliográficos
Autor(a) principal: Palomares-Jerez, M. Francisca
Data de Publicação: 2013
Outros Autores: Nemesio, Henrique, Franquelim, Henri G., Castanho, Miguel A. R. B., Villalaín, José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10690
Resumo: © 2013 Elsevier B.V. All rights reserved.
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spelling N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranesHCVNS4BPhospholipidMembrane© 2013 Elsevier B.V. All rights reserved.HCV NS4B, a highly hydrophobic protein involved in the alteration of the intracellular host membranes forming the replication complex, plays a critical role in the HCV life cycle. NS4B is a multifunctional membrane protein that possesses different regions where diverse and significant functions are located. One of these important regions is the AH2 segment, which besides being highly conserved has been shown to play a significant role in NS4B functioning. We have carried out an in-depth biophysical study aimed at the elucidation of the capacity of this region to interact, modulate and disrupt membranes, as well as to study the structural and dynamic features relevant for that disruption. We show that a peptide derived from this region, NS4BAH2, is capable of specifically binding phosphatidyl inositol phosphates with high affinity, and its interfacial properties suggest that this segment could behave similarly to a pre-transmembrane domain partitioning into and interacting with the membrane depending on the membrane composition and/or other proteins. Moreover, NS4BAH2 is capable of rupturing membranes even at very low peptide-to-lipid ratios and its membrane-activity is modulated by lipid composition. NS4BAH2 is located in a shallow position in the membrane but it is able to affect the lipid environment from the membrane surface down to the hydrophobic core. The NS4B region where peptide NS4BAH2 resides might have an essential role in the membrane replication and/or assembly of the viral particle through the modulation of the membrane structure and hence the replication complex.This work was partially supported by grants REEQ/140/BIO/2005, PTDC/QUI-BIQ/114774/2009 and PTDC/QUI-BIQ/112929/2009 to M.R.B.C (FCT-MES, Portugal). H. N. is supported by a “Santiago Grisolía” fellowship from Generalitat Valenciana Autonomous Government, Spain. F.P.J. acknowledges FEBS for a Short Term Fellowship.ElsevierRepositório da Universidade de LisboaPalomares-Jerez, M. FranciscaNemesio, HenriqueFranquelim, Henri G.Castanho, Miguel A. R. B.Villalaín, José2014-03-06T11:17:20Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10690engBiochimica et Biophysica Acta 1828 (2013) 1938–19520006-3002http://dx.doi.org/10.1016/j.bbamem.2013.04.020metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:56:21Zoai:repositorio.ul.pt:10451/10690Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:34:37.827361Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranes
title N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranes
spellingShingle N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranes
Palomares-Jerez, M. Francisca
HCV
NS4B
Phospholipid
Membrane
title_short N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranes
title_full N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranes
title_fullStr N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranes
title_full_unstemmed N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranes
title_sort N-terminal AH2 segment of protein NS4B from hepatitis C virus : binding to and interaction with model biomembranes
author Palomares-Jerez, M. Francisca
author_facet Palomares-Jerez, M. Francisca
Nemesio, Henrique
Franquelim, Henri G.
Castanho, Miguel A. R. B.
Villalaín, José
author_role author
author2 Nemesio, Henrique
Franquelim, Henri G.
Castanho, Miguel A. R. B.
Villalaín, José
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Palomares-Jerez, M. Francisca
Nemesio, Henrique
Franquelim, Henri G.
Castanho, Miguel A. R. B.
Villalaín, José
dc.subject.por.fl_str_mv HCV
NS4B
Phospholipid
Membrane
topic HCV
NS4B
Phospholipid
Membrane
description © 2013 Elsevier B.V. All rights reserved.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-03-06T11:17:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10690
url http://hdl.handle.net/10451/10690
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochimica et Biophysica Acta 1828 (2013) 1938–1952
0006-3002
http://dx.doi.org/10.1016/j.bbamem.2013.04.020
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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