Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach

Detalhes bibliográficos
Autor(a) principal: Andrade, Claúdia
Data de Publicação: 2021
Outros Autores: Oliveira, Bárbara, Guatelli, Silvia, Martinez, Pedro, Simões, Beatriz, Bispo, Claúdia, Ferrario, Cinzia, Bonasoro, Francesco, Rino, José, Sugni, Michela, Gardner, Rui, Zilhão, Rita, Coelho, Ana Varela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116404
Resumo: Funding: To the “Maristem COST Action” (CA16203), supported by COST (European Cooperation in Science and Technology), for funding PM STSM visits (February 2019 and November 2020) to the AVC Laboratory.
id RCAP_4105908c061139711facbd3a34631192
oai_identifier_str oai:run.unl.pt:10362/116404
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approachcoelomocytesechinodermsflow cytometry and imaging flow cytometryfluorescence and transmission electron microscopyMarthasterias glacialisImmunology and AllergyImmunologyFunding: To the “Maristem COST Action” (CA16203), supported by COST (European Cooperation in Science and Technology), for funding PM STSM visits (February 2019 and November 2020) to the AVC Laboratory.Coelomocytes is the generic name for a collection of cellular morphotypes, present in many coelomate animals, and highly variable among echinoderm classes. The roles attributed to the major types of these free circulating cells present in the coelomic fluid of echinoderms include immune response, phagocytic digestion and clotting. Our main aim in this study was to characterize coelomocytes found in the coelomic fluid of Marthasterias glacialis (class Asteroidea) by using a combination of flow cytometry (FC), imaging flow cytometry (IFC) and fluorescence plus transmission electron microscopy (TEM). Two coelomocyte populations (P1 and P2) identified through flow cytometry were subsequently studied in terms of abundance, morphology, ultrastructure, cell viability and cell cycle profiles. Ultrastructurally, P2 diploid cells were present as two main morphotypes, similar to phagocytes and vertebrate thrombocytes, whereas the smaller P1 cellular population was characterized by low mitotic activity, a relatively undifferentiated cytotype and a high nucleus/cytoplasm ratio. In the present study we could not rule out possible similarities between haploid P1 cells and stem-cell types in other animals. Additionally, we report the presence of two other morphotypes in P2 that could only be detected by fluorescence microscopy, as well as a morphotype revealed via combined microscopy/FC. This integrative experimental workflow combined cells physical separation with different microscopic image capture technologies, enabling us to better tackle the characterization of the heterogeneous composition of coelomocytes populations.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNAndrade, ClaúdiaOliveira, BárbaraGuatelli, SilviaMartinez, PedroSimões, BeatrizBispo, ClaúdiaFerrario, CinziaBonasoro, FrancescoRino, JoséSugni, MichelaGardner, RuiZilhão, RitaCoelho, Ana Varela2021-04-29T23:03:14Z2021-03-182021-03-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/116404eng1664-3224PURE: 29165320https://doi.org/10.3389/fimmu.2021.641664info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:58:51Zoai:run.unl.pt:10362/116404Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:02.946384Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach
title Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach
spellingShingle Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach
Andrade, Claúdia
coelomocytes
echinoderms
flow cytometry and imaging flow cytometry
fluorescence and transmission electron microscopy
Marthasterias glacialis
Immunology and Allergy
Immunology
title_short Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach
title_full Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach
title_fullStr Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach
title_full_unstemmed Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach
title_sort Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach
author Andrade, Claúdia
author_facet Andrade, Claúdia
Oliveira, Bárbara
Guatelli, Silvia
Martinez, Pedro
Simões, Beatriz
Bispo, Claúdia
Ferrario, Cinzia
Bonasoro, Francesco
Rino, José
Sugni, Michela
Gardner, Rui
Zilhão, Rita
Coelho, Ana Varela
author_role author
author2 Oliveira, Bárbara
Guatelli, Silvia
Martinez, Pedro
Simões, Beatriz
Bispo, Claúdia
Ferrario, Cinzia
Bonasoro, Francesco
Rino, José
Sugni, Michela
Gardner, Rui
Zilhão, Rita
Coelho, Ana Varela
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Andrade, Claúdia
Oliveira, Bárbara
Guatelli, Silvia
Martinez, Pedro
Simões, Beatriz
Bispo, Claúdia
Ferrario, Cinzia
Bonasoro, Francesco
Rino, José
Sugni, Michela
Gardner, Rui
Zilhão, Rita
Coelho, Ana Varela
dc.subject.por.fl_str_mv coelomocytes
echinoderms
flow cytometry and imaging flow cytometry
fluorescence and transmission electron microscopy
Marthasterias glacialis
Immunology and Allergy
Immunology
topic coelomocytes
echinoderms
flow cytometry and imaging flow cytometry
fluorescence and transmission electron microscopy
Marthasterias glacialis
Immunology and Allergy
Immunology
description Funding: To the “Maristem COST Action” (CA16203), supported by COST (European Cooperation in Science and Technology), for funding PM STSM visits (February 2019 and November 2020) to the AVC Laboratory.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-29T23:03:14Z
2021-03-18
2021-03-18T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116404
url http://hdl.handle.net/10362/116404
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-3224
PURE: 29165320
https://doi.org/10.3389/fimmu.2021.641664
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138041198018560

Registros relacionados