Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/116404 |
Resumo: | Funding: To the “Maristem COST Action” (CA16203), supported by COST (European Cooperation in Science and Technology), for funding PM STSM visits (February 2019 and November 2020) to the AVC Laboratory. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approachcoelomocytesechinodermsflow cytometry and imaging flow cytometryfluorescence and transmission electron microscopyMarthasterias glacialisImmunology and AllergyImmunologyFunding: To the “Maristem COST Action” (CA16203), supported by COST (European Cooperation in Science and Technology), for funding PM STSM visits (February 2019 and November 2020) to the AVC Laboratory.Coelomocytes is the generic name for a collection of cellular morphotypes, present in many coelomate animals, and highly variable among echinoderm classes. The roles attributed to the major types of these free circulating cells present in the coelomic fluid of echinoderms include immune response, phagocytic digestion and clotting. Our main aim in this study was to characterize coelomocytes found in the coelomic fluid of Marthasterias glacialis (class Asteroidea) by using a combination of flow cytometry (FC), imaging flow cytometry (IFC) and fluorescence plus transmission electron microscopy (TEM). Two coelomocyte populations (P1 and P2) identified through flow cytometry were subsequently studied in terms of abundance, morphology, ultrastructure, cell viability and cell cycle profiles. Ultrastructurally, P2 diploid cells were present as two main morphotypes, similar to phagocytes and vertebrate thrombocytes, whereas the smaller P1 cellular population was characterized by low mitotic activity, a relatively undifferentiated cytotype and a high nucleus/cytoplasm ratio. In the present study we could not rule out possible similarities between haploid P1 cells and stem-cell types in other animals. Additionally, we report the presence of two other morphotypes in P2 that could only be detected by fluorescence microscopy, as well as a morphotype revealed via combined microscopy/FC. This integrative experimental workflow combined cells physical separation with different microscopic image capture technologies, enabling us to better tackle the characterization of the heterogeneous composition of coelomocytes populations.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNAndrade, ClaúdiaOliveira, BárbaraGuatelli, SilviaMartinez, PedroSimões, BeatrizBispo, ClaúdiaFerrario, CinziaBonasoro, FrancescoRino, JoséSugni, MichelaGardner, RuiZilhão, RitaCoelho, Ana Varela2021-04-29T23:03:14Z2021-03-182021-03-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/116404eng1664-3224PURE: 29165320https://doi.org/10.3389/fimmu.2021.641664info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:58:51Zoai:run.unl.pt:10362/116404Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:02.946384Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach |
title |
Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach |
spellingShingle |
Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach Andrade, Claúdia coelomocytes echinoderms flow cytometry and imaging flow cytometry fluorescence and transmission electron microscopy Marthasterias glacialis Immunology and Allergy Immunology |
title_short |
Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach |
title_full |
Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach |
title_fullStr |
Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach |
title_full_unstemmed |
Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach |
title_sort |
Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach |
author |
Andrade, Claúdia |
author_facet |
Andrade, Claúdia Oliveira, Bárbara Guatelli, Silvia Martinez, Pedro Simões, Beatriz Bispo, Claúdia Ferrario, Cinzia Bonasoro, Francesco Rino, José Sugni, Michela Gardner, Rui Zilhão, Rita Coelho, Ana Varela |
author_role |
author |
author2 |
Oliveira, Bárbara Guatelli, Silvia Martinez, Pedro Simões, Beatriz Bispo, Claúdia Ferrario, Cinzia Bonasoro, Francesco Rino, José Sugni, Michela Gardner, Rui Zilhão, Rita Coelho, Ana Varela |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
Andrade, Claúdia Oliveira, Bárbara Guatelli, Silvia Martinez, Pedro Simões, Beatriz Bispo, Claúdia Ferrario, Cinzia Bonasoro, Francesco Rino, José Sugni, Michela Gardner, Rui Zilhão, Rita Coelho, Ana Varela |
dc.subject.por.fl_str_mv |
coelomocytes echinoderms flow cytometry and imaging flow cytometry fluorescence and transmission electron microscopy Marthasterias glacialis Immunology and Allergy Immunology |
topic |
coelomocytes echinoderms flow cytometry and imaging flow cytometry fluorescence and transmission electron microscopy Marthasterias glacialis Immunology and Allergy Immunology |
description |
Funding: To the “Maristem COST Action” (CA16203), supported by COST (European Cooperation in Science and Technology), for funding PM STSM visits (February 2019 and November 2020) to the AVC Laboratory. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-29T23:03:14Z 2021-03-18 2021-03-18T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/116404 |
url |
http://hdl.handle.net/10362/116404 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1664-3224 PURE: 29165320 https://doi.org/10.3389/fimmu.2021.641664 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138041198018560 |