Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)

Detalhes bibliográficos
Autor(a) principal: Sabença, Carolina
Data de Publicação: 2020
Outros Autores: de Sousa, Telma, Oliveira, Soraia, Viala, Didier, Théron, Laetitia, Chambon, Christophe, Hébraud, Michel, Beyrouthy, Racha, Bonnet, Richard, Caniça, Manuela, Poeta, Patrícia, Igrejas, Gilberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/120027
Resumo: Ação n.º: TC-14 / 2017 UID/QUI/50006/2020 UIDB/00211/2020 UMR1071 USC-2018
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spelling Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)Antibiotic resistanceEnterococcus sppMALDI-TOF MSNext-generation sequencingProcessed meatBiochemistry, Genetics and Molecular Biology(all)Immunology and Microbiology(all)Agricultural and Biological Sciences(all)Ação n.º: TC-14 / 2017 UID/QUI/50006/2020 UIDB/00211/2020 UMR1071 USC-2018Vancomycin-resistant enterococci (VRE), due to their intrinsic resistance to various commonly used antibiotics and their malleable genome, make the treatment of infections caused by these bacteria less effective. The aims of this work were to characterize isolates of Enterococcus spp. that originated from processed meat, through phenotypic and genotypic techniques, as well as to detect putative antibiotic resistance biomarkers. The 19 VRE identified had high resistance to teicoplanin (89%), tetracycline (94%), and erythromycin (84%) and a low resistance to kanamycin (11%), gentamicin (11%), and streptomycin (5%). Based on a Next-Generation Sequencing NGS technique, most isolates were vanA-positive. The most prevalent resistance genes detected were erm(B) and aac(6')-Ii, conferring resistance to the classes of macrolides and aminoglycosides, respectively. MALDI-TOF mass spectrometry (MS) analysis detected an exclusive peak of the Enterococcus genus at m/z (mass-to-charge-ratio) 4428 ± 3, and a peak at m/z 6048 ± 1 allowed us to distinguish Enterococcus faecium from the other species. Several statistically significant protein masses associated with resistance were detected, such as peaks at m/z 6358.27 and m/z 13237.3 in ciprofloxacin resistance isolates. These results reinforce the relevance of the combined and complementary NGS and MALDI-TOF MS techniques for bacterial characterization.LAQV@REQUIMTERUNSabença, Carolinade Sousa, TelmaOliveira, SoraiaViala, DidierThéron, LaetitiaChambon, ChristopheHébraud, MichelBeyrouthy, RachaBonnet, RichardCaniça, ManuelaPoeta, PatríciaIgrejas, Gilberto2021-06-25T22:18:36Z2020-052020-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/120027engPURE: 32184625https://doi.org/10.3390/biology9050089info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:54:13Zoai:run.unl.pt:10362/120027Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:54:13Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)
title Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)
spellingShingle Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)
Sabença, Carolina
Antibiotic resistance
Enterococcus spp
MALDI-TOF MS
Next-generation sequencing
Processed meat
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Agricultural and Biological Sciences(all)
title_short Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)
title_full Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)
title_fullStr Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)
title_full_unstemmed Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)
title_sort Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)
author Sabença, Carolina
author_facet Sabença, Carolina
de Sousa, Telma
Oliveira, Soraia
Viala, Didier
Théron, Laetitia
Chambon, Christophe
Hébraud, Michel
Beyrouthy, Racha
Bonnet, Richard
Caniça, Manuela
Poeta, Patrícia
Igrejas, Gilberto
author_role author
author2 de Sousa, Telma
Oliveira, Soraia
Viala, Didier
Théron, Laetitia
Chambon, Christophe
Hébraud, Michel
Beyrouthy, Racha
Bonnet, Richard
Caniça, Manuela
Poeta, Patrícia
Igrejas, Gilberto
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
RUN
dc.contributor.author.fl_str_mv Sabença, Carolina
de Sousa, Telma
Oliveira, Soraia
Viala, Didier
Théron, Laetitia
Chambon, Christophe
Hébraud, Michel
Beyrouthy, Racha
Bonnet, Richard
Caniça, Manuela
Poeta, Patrícia
Igrejas, Gilberto
dc.subject.por.fl_str_mv Antibiotic resistance
Enterococcus spp
MALDI-TOF MS
Next-generation sequencing
Processed meat
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Agricultural and Biological Sciences(all)
topic Antibiotic resistance
Enterococcus spp
MALDI-TOF MS
Next-generation sequencing
Processed meat
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Agricultural and Biological Sciences(all)
description Ação n.º: TC-14 / 2017 UID/QUI/50006/2020 UIDB/00211/2020 UMR1071 USC-2018
publishDate 2020
dc.date.none.fl_str_mv 2020-05
2020-05-01T00:00:00Z
2021-06-25T22:18:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/120027
url http://hdl.handle.net/10362/120027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 32184625
https://doi.org/10.3390/biology9050089
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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