Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10216/114485 |
Resumo: | Listeria monocytogenes (Lm) is a human intracellular pathogen widely used to uncover the mechanisms evolved by pathogens to establish infection. However, its capacity to perturb the host cell cycle was never reported. We show that Lm infection affects the host cell cycle progression, increasing its overall duration but allowing consecutive rounds of division. A complete Lm infectious cycle induces a S-phase delay accompanied by a slower rate of DNA synthesis and increased levels of host DNA strand breaks. Additionally, DNA damage/replication checkpoint responses are triggered in an Lm dose-dependent manner through the phosphorylation of DNA-PK, H2A.X, and CDC25A and independently from ATM/ATR. While host DNA damage induced exogenously favors Lm dissemination, the override of checkpoint pathways limits infection. We propose that host DNA replication disturbed by Lm infection culminates in DNA strand breaks, triggering DNA damage/replication responses, and ensuring a cell cycle delay that favors Lm propagation. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infectionCalcium-Binding Proteins/metabolismCell Cycle/physiologyCell Line, TumorDNA BreaksDNA DamageDNA RepairDNA ReplicationHost-Pathogen InteractionsHumansListeria monocytogenes/physiologyListeriosis/metabolismListeriosis/microbiologyListeriosis/pathologyS Phasecdc25 Phosphatases/metabolismListeria monocytogenes (Lm) is a human intracellular pathogen widely used to uncover the mechanisms evolved by pathogens to establish infection. However, its capacity to perturb the host cell cycle was never reported. We show that Lm infection affects the host cell cycle progression, increasing its overall duration but allowing consecutive rounds of division. A complete Lm infectious cycle induces a S-phase delay accompanied by a slower rate of DNA synthesis and increased levels of host DNA strand breaks. Additionally, DNA damage/replication checkpoint responses are triggered in an Lm dose-dependent manner through the phosphorylation of DNA-PK, H2A.X, and CDC25A and independently from ATM/ATR. While host DNA damage induced exogenously favors Lm dissemination, the override of checkpoint pathways limits infection. We propose that host DNA replication disturbed by Lm infection culminates in DNA strand breaks, triggering DNA damage/replication responses, and ensuring a cell cycle delay that favors Lm propagation.Taylor & Francis20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10216/114485eng1538-410110.4161/cc.27780Leitão, ECosta, ACBrito, CCosta, LPombinho, RCabanes, DSousa, Sinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T12:29:23Zoai:repositorio-aberto.up.pt:10216/114485Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:21:16.748487Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection |
title |
Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection |
spellingShingle |
Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection Leitão, E Calcium-Binding Proteins/metabolism Cell Cycle/physiology Cell Line, Tumor DNA Breaks DNA Damage DNA Repair DNA Replication Host-Pathogen Interactions Humans Listeria monocytogenes/physiology Listeriosis/metabolism Listeriosis/microbiology Listeriosis/pathology S Phase cdc25 Phosphatases/metabolism |
title_short |
Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection |
title_full |
Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection |
title_fullStr |
Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection |
title_full_unstemmed |
Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection |
title_sort |
Listeria monocytogenes induces host DNA damage and delays the host cell cycle to promote infection |
author |
Leitão, E |
author_facet |
Leitão, E Costa, AC Brito, C Costa, L Pombinho, R Cabanes, D Sousa, S |
author_role |
author |
author2 |
Costa, AC Brito, C Costa, L Pombinho, R Cabanes, D Sousa, S |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Leitão, E Costa, AC Brito, C Costa, L Pombinho, R Cabanes, D Sousa, S |
dc.subject.por.fl_str_mv |
Calcium-Binding Proteins/metabolism Cell Cycle/physiology Cell Line, Tumor DNA Breaks DNA Damage DNA Repair DNA Replication Host-Pathogen Interactions Humans Listeria monocytogenes/physiology Listeriosis/metabolism Listeriosis/microbiology Listeriosis/pathology S Phase cdc25 Phosphatases/metabolism |
topic |
Calcium-Binding Proteins/metabolism Cell Cycle/physiology Cell Line, Tumor DNA Breaks DNA Damage DNA Repair DNA Replication Host-Pathogen Interactions Humans Listeria monocytogenes/physiology Listeriosis/metabolism Listeriosis/microbiology Listeriosis/pathology S Phase cdc25 Phosphatases/metabolism |
description |
Listeria monocytogenes (Lm) is a human intracellular pathogen widely used to uncover the mechanisms evolved by pathogens to establish infection. However, its capacity to perturb the host cell cycle was never reported. We show that Lm infection affects the host cell cycle progression, increasing its overall duration but allowing consecutive rounds of division. A complete Lm infectious cycle induces a S-phase delay accompanied by a slower rate of DNA synthesis and increased levels of host DNA strand breaks. Additionally, DNA damage/replication checkpoint responses are triggered in an Lm dose-dependent manner through the phosphorylation of DNA-PK, H2A.X, and CDC25A and independently from ATM/ATR. While host DNA damage induced exogenously favors Lm dissemination, the override of checkpoint pathways limits infection. We propose that host DNA replication disturbed by Lm infection culminates in DNA strand breaks, triggering DNA damage/replication responses, and ensuring a cell cycle delay that favors Lm propagation. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10216/114485 |
url |
http://hdl.handle.net/10216/114485 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1538-4101 10.4161/cc.27780 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799135512519245824 |