Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis

Detalhes bibliográficos
Autor(a) principal: Tarafder, Abul K.
Data de Publicação: 2014
Outros Autores: Bolasco, Giulia, Correia, Maria S., Pereira, Francisco J.C., Iannone, Lucio, Hume, Alistair N., Kirkpatrick, Niall, Picardo, Mauro, Torrisi, Maria R., Rodrigues, Inês P., Ramalho, José S., Futter, Clare E., Barral, Duarte C., Seabra, Miguel C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/161728
Resumo: Funding Information: We thank Peter Munro (University College London) for his assistance with high-pressure freezing and freeze substitution. This work was supported by the Welcome Trust (0575498/Z/04/Z), BBSRC (BB/E021689/1), and Fundação para a Ciência e a Tecnologia (PTDC/BIA-BCM/111735/2009 and SFRH/BD/65381/2009).
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spelling Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosisBiochemistryMolecular BiologyDermatologyCell BiologyFunding Information: We thank Peter Munro (University College London) for his assistance with high-pressure freezing and freeze substitution. This work was supported by the Welcome Trust (0575498/Z/04/Z), BBSRC (BB/E021689/1), and Fundação para a Ciência e a Tecnologia (PTDC/BIA-BCM/111735/2009 and SFRH/BD/65381/2009).The transfer of melanin from melanocytes to keratinocytes is a crucial process underlying maintenance of skin pigmentation and photoprotection against UV damage. Here, we present evidence supporting coupled exocytosis of the melanin core, or melanocore, by melanocytes and subsequent endocytosis by keratinocytes as a predominant mechanism of melanin transfer. Electron microscopy analysis of human skin samples revealed three lines of evidence supporting this: (1) the presence of melanocores in the extracellular space; (2) within keratinocytes, melanin was surrounded by a single membrane; and (3) this membrane lacked the melanosomal membrane protein tyrosinase-related protein 1 (TYRP1). Moreover, co-culture of melanocytes and keratinocytes suggests that melanin exocytosis is specifically induced by keratinocytes. Furthermore, depletion of Rab11b, but not Rab27a, caused a marked decrease in both keratinocyte-stimulated melanin exocytosis and transfer to keratinocytes. Thus, we propose that the predominant mechanism of melanin transfer is keratinocyte-induced exocytosis, mediated by Rab11b through remodeling of the melanosome membrane, followed by subsequent endocytosis by keratinocytes.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNTarafder, Abul K.Bolasco, GiuliaCorreia, Maria S.Pereira, Francisco J.C.Iannone, LucioHume, Alistair N.Kirkpatrick, NiallPicardo, MauroTorrisi, Maria R.Rodrigues, Inês P.Ramalho, José S.Futter, Clare E.Barral, Duarte C.Seabra, Miguel C.2023-12-28T22:33:33Z2014-042014-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/161728eng0022-202XPURE: 79336112https://doi.org/10.1038/jid.2013.432info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:44:36Zoai:run.unl.pt:10362/161728Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:37.918101Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis
title Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis
spellingShingle Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis
Tarafder, Abul K.
Biochemistry
Molecular Biology
Dermatology
Cell Biology
title_short Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis
title_full Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis
title_fullStr Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis
title_full_unstemmed Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis
title_sort Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis
author Tarafder, Abul K.
author_facet Tarafder, Abul K.
Bolasco, Giulia
Correia, Maria S.
Pereira, Francisco J.C.
Iannone, Lucio
Hume, Alistair N.
Kirkpatrick, Niall
Picardo, Mauro
Torrisi, Maria R.
Rodrigues, Inês P.
Ramalho, José S.
Futter, Clare E.
Barral, Duarte C.
Seabra, Miguel C.
author_role author
author2 Bolasco, Giulia
Correia, Maria S.
Pereira, Francisco J.C.
Iannone, Lucio
Hume, Alistair N.
Kirkpatrick, Niall
Picardo, Mauro
Torrisi, Maria R.
Rodrigues, Inês P.
Ramalho, José S.
Futter, Clare E.
Barral, Duarte C.
Seabra, Miguel C.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
RUN
dc.contributor.author.fl_str_mv Tarafder, Abul K.
Bolasco, Giulia
Correia, Maria S.
Pereira, Francisco J.C.
Iannone, Lucio
Hume, Alistair N.
Kirkpatrick, Niall
Picardo, Mauro
Torrisi, Maria R.
Rodrigues, Inês P.
Ramalho, José S.
Futter, Clare E.
Barral, Duarte C.
Seabra, Miguel C.
dc.subject.por.fl_str_mv Biochemistry
Molecular Biology
Dermatology
Cell Biology
topic Biochemistry
Molecular Biology
Dermatology
Cell Biology
description Funding Information: We thank Peter Munro (University College London) for his assistance with high-pressure freezing and freeze substitution. This work was supported by the Welcome Trust (0575498/Z/04/Z), BBSRC (BB/E021689/1), and Fundação para a Ciência e a Tecnologia (PTDC/BIA-BCM/111735/2009 and SFRH/BD/65381/2009).
publishDate 2014
dc.date.none.fl_str_mv 2014-04
2014-04-01T00:00:00Z
2023-12-28T22:33:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/161728
url http://hdl.handle.net/10362/161728
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0022-202X
PURE: 79336112
https://doi.org/10.1038/jid.2013.432
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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