Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3

Detalhes bibliográficos
Autor(a) principal: Almeida, Rui M.
Data de Publicação: 2011
Outros Autores: Geraldes, Carlos F. G. C., Pauleta, Sofia R., Moura, José J. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8610
Resumo: Inorganic Chemistry 50(21):10600-7
id RCAP_4203280f39ecd1693ee4d83c4d2eee16
oai_identifier_str oai:run.unl.pt:10362/8610
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3Inorganic Chemistry 50(21):10600-7Two cyclen-derived Gd probes, [Gd-DOTAM](3+) and [Gd-DOTP](5-) (DOTAM = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetamide; DOTP = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylenephosphonate)), were assessed as paramagnetic relaxation enhancement (PRE)-inducing probes for characterization of protein-protein interactions. Two proteins, Desulfovibrio gigas rubredoxin and Desulfovibrio gigas cytochrome c(3), were used as model partners. In a (1)H NMR titration it was shown that [Gd-DOTP](5-) binds to cytochrome c(3) near heme IV, causing pronounced PREs, characterized by line width broadenings of the heme methyl resonances at ratios as low as 0.08. A K(d) of 23 ± 1 μM was calculated based on chemical shift perturbation of selected heme methyl resonances belonging to three different heme groups, caused by allosteric effects upon [Gd-DOTP](5-) binding to cytochrome c(3) at a molar ratio of 2. The other probe, [Gd-DOTAM](3+), caused PREs on a well-defined patch near the metal center of rubredoxin (especially the patch constituted by residues D19-G23 and W37-S45, which broaden beyond detection). This effect was partially reversed for some resonances (C6-Y11, in particular) when cytochrome c(3) was added to this system. Both probes were successful in causing reversible PREs at the partner binding site, thus showing to be good probes to identify partners' binding sites and since the interaction is reversible to structurally characterize protein complexes by better defining the complex interface.American Chemical SocietyRUNAlmeida, Rui M.Geraldes, Carlos F. G. C.Pauleta, Sofia R.Moura, José J. G.2013-01-30T09:50:15Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8610eng0020-1669info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:25Zoai:run.unl.pt:10362/8610Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:19.050981Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3
title Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3
spellingShingle Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3
Almeida, Rui M.
title_short Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3
title_full Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3
title_fullStr Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3
title_full_unstemmed Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3
title_sort Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3
author Almeida, Rui M.
author_facet Almeida, Rui M.
Geraldes, Carlos F. G. C.
Pauleta, Sofia R.
Moura, José J. G.
author_role author
author2 Geraldes, Carlos F. G. C.
Pauleta, Sofia R.
Moura, José J. G.
author2_role author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Almeida, Rui M.
Geraldes, Carlos F. G. C.
Pauleta, Sofia R.
Moura, José J. G.
description Inorganic Chemistry 50(21):10600-7
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2013-01-30T09:50:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8610
url http://hdl.handle.net/10362/8610
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0020-1669
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137829149736960

Registros relacionados