Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/8610 |
Resumo: | Inorganic Chemistry 50(21):10600-7 |
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Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3Inorganic Chemistry 50(21):10600-7Two cyclen-derived Gd probes, [Gd-DOTAM](3+) and [Gd-DOTP](5-) (DOTAM = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetamide; DOTP = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylenephosphonate)), were assessed as paramagnetic relaxation enhancement (PRE)-inducing probes for characterization of protein-protein interactions. Two proteins, Desulfovibrio gigas rubredoxin and Desulfovibrio gigas cytochrome c(3), were used as model partners. In a (1)H NMR titration it was shown that [Gd-DOTP](5-) binds to cytochrome c(3) near heme IV, causing pronounced PREs, characterized by line width broadenings of the heme methyl resonances at ratios as low as 0.08. A K(d) of 23 ± 1 μM was calculated based on chemical shift perturbation of selected heme methyl resonances belonging to three different heme groups, caused by allosteric effects upon [Gd-DOTP](5-) binding to cytochrome c(3) at a molar ratio of 2. The other probe, [Gd-DOTAM](3+), caused PREs on a well-defined patch near the metal center of rubredoxin (especially the patch constituted by residues D19-G23 and W37-S45, which broaden beyond detection). This effect was partially reversed for some resonances (C6-Y11, in particular) when cytochrome c(3) was added to this system. Both probes were successful in causing reversible PREs at the partner binding site, thus showing to be good probes to identify partners' binding sites and since the interaction is reversible to structurally characterize protein complexes by better defining the complex interface.American Chemical SocietyRUNAlmeida, Rui M.Geraldes, Carlos F. G. C.Pauleta, Sofia R.Moura, José J. G.2013-01-30T09:50:15Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8610eng0020-1669info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:25Zoai:run.unl.pt:10362/8610Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:19.050981Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3 |
title |
Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3 |
spellingShingle |
Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3 Almeida, Rui M. |
title_short |
Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3 |
title_full |
Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3 |
title_fullStr |
Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3 |
title_full_unstemmed |
Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3 |
title_sort |
Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rubredoxin and cytochrome c3 |
author |
Almeida, Rui M. |
author_facet |
Almeida, Rui M. Geraldes, Carlos F. G. C. Pauleta, Sofia R. Moura, José J. G. |
author_role |
author |
author2 |
Geraldes, Carlos F. G. C. Pauleta, Sofia R. Moura, José J. G. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
RUN |
dc.contributor.author.fl_str_mv |
Almeida, Rui M. Geraldes, Carlos F. G. C. Pauleta, Sofia R. Moura, José J. G. |
description |
Inorganic Chemistry 50(21):10600-7 |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z 2013-01-30T09:50:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/8610 |
url |
http://hdl.handle.net/10362/8610 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0020-1669 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137829149736960 |