A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection

Detalhes bibliográficos
Autor(a) principal: Kitowski, Annabel Katharina
Data de Publicação: 2020
Outros Autores: Bernardes, Gonçalo J. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/50034
Resumo: © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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spelling A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infectionNioorthogonal chemistryCarbohydratesGLUT1 transportersInverse electron-demand Diels-AlderMalaria© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation. We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria. Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells. Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former. This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.This project has received funding from the European Union's Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement no. 675671 (A.K. and G.J.L.B.). Funding from the Royal Society (URF\R\180019 to G.J.L.B.) and FCT Portugal (iFCT IF/00624/2015 to G.J.L.B.) is also acknowledged.John Wiley & Sons, Inc.Repositório da Universidade de LisboaKitowski, Annabel KatharinaBernardes, Gonçalo J. L.2021-10-27T14:21:13Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/50034engChembiochem. 2020 Sep 14;21(18):2696-27001439-422710.1002/cbic.2020002261439-7633info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:54:03Zoai:repositorio.ul.pt:10451/50034Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:33.260663Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
title A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
spellingShingle A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
Kitowski, Annabel Katharina
Nioorthogonal chemistry
Carbohydrates
GLUT1 transporters
Inverse electron-demand Diels-Alder
Malaria
title_short A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
title_full A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
title_fullStr A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
title_full_unstemmed A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
title_sort A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
author Kitowski, Annabel Katharina
author_facet Kitowski, Annabel Katharina
Bernardes, Gonçalo J. L.
author_role author
author2 Bernardes, Gonçalo J. L.
author2_role author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Kitowski, Annabel Katharina
Bernardes, Gonçalo J. L.
dc.subject.por.fl_str_mv Nioorthogonal chemistry
Carbohydrates
GLUT1 transporters
Inverse electron-demand Diels-Alder
Malaria
topic Nioorthogonal chemistry
Carbohydrates
GLUT1 transporters
Inverse electron-demand Diels-Alder
Malaria
description © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-10-27T14:21:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/50034
url http://hdl.handle.net/10451/50034
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chembiochem. 2020 Sep 14;21(18):2696-2700
1439-4227
10.1002/cbic.202000226
1439-7633
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons, Inc.
publisher.none.fl_str_mv John Wiley & Sons, Inc.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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