A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/50034 |
Resumo: | © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infectionNioorthogonal chemistryCarbohydratesGLUT1 transportersInverse electron-demand Diels-AlderMalaria© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation. We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria. Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells. Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former. This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.This project has received funding from the European Union's Horizon 2020 research and innovation programme under Marie Skłodowska-Curie grant agreement no. 675671 (A.K. and G.J.L.B.). Funding from the Royal Society (URF\R\180019 to G.J.L.B.) and FCT Portugal (iFCT IF/00624/2015 to G.J.L.B.) is also acknowledged.John Wiley & Sons, Inc.Repositório da Universidade de LisboaKitowski, Annabel KatharinaBernardes, Gonçalo J. L.2021-10-27T14:21:13Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/50034engChembiochem. 2020 Sep 14;21(18):2696-27001439-422710.1002/cbic.2020002261439-7633info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:54:03Zoai:repositorio.ul.pt:10451/50034Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:33.260663Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection |
title |
A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection |
spellingShingle |
A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection Kitowski, Annabel Katharina Nioorthogonal chemistry Carbohydrates GLUT1 transporters Inverse electron-demand Diels-Alder Malaria |
title_short |
A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection |
title_full |
A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection |
title_fullStr |
A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection |
title_full_unstemmed |
A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection |
title_sort |
A sweet galactose transfer: metabolic oligosaccharide engineering as a tool to study glycans in plasmodium infection |
author |
Kitowski, Annabel Katharina |
author_facet |
Kitowski, Annabel Katharina Bernardes, Gonçalo J. L. |
author_role |
author |
author2 |
Bernardes, Gonçalo J. L. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Kitowski, Annabel Katharina Bernardes, Gonçalo J. L. |
dc.subject.por.fl_str_mv |
Nioorthogonal chemistry Carbohydrates GLUT1 transporters Inverse electron-demand Diels-Alder Malaria |
topic |
Nioorthogonal chemistry Carbohydrates GLUT1 transporters Inverse electron-demand Diels-Alder Malaria |
description |
© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-10-27T14:21:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/50034 |
url |
http://hdl.handle.net/10451/50034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chembiochem. 2020 Sep 14;21(18):2696-2700 1439-4227 10.1002/cbic.202000226 1439-7633 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley & Sons, Inc. |
publisher.none.fl_str_mv |
John Wiley & Sons, Inc. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799134563733078016 |