In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: de Freitas Oliveira, Johny Wysllas
Data de Publicação: 2022
Outros Autores: da Silva, Mariana Farias Alves, Damasceno, Igor Zumba, Rocha, Hugo Alexandre Oliveira, da Silva Júnior, Arnóbio Antônio, Silva, Marcelo Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/147867
Resumo: Funding Information: This research was funded by Global Health and Tropical Medicine: Grant number IHMT-UID/multi/04413/2013 and Grant number PTDC/CVT-CVT/28908/2017, FCT-Portugal.We would like to thank João Aristeu da Rosa and Aline Rimodi Rimeiro at UNESP (Universidade Estadual de São Paulo) Araraquara (Brazil) for offering four different strains of T. cruzi. JWFO and MFAS give thanks to their financial support (PhD and post-doctoral fellowships) provided by Capes/Brazil; MSS, AASJ, and HAOR give thanks to CNPq/Brazil for the Research Grant (Bolsa de Produtividade). We would also like to thank the Department of Materials Engineering at UFRN for affording us the use of their scanning electron microscope, and the Department of Biochemistry at UFRN for affording us the use of their culture room. We thank Laysa Ohana for reviewing and editing the English version of the manuscript. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruziChagas diseaseNanoparticleNanoprecipitationSodium diethyldithiocarbamateTrypanosoma cruziQR MicrobiologyQD ChemistryPharmaceutical ScienceParasitologyDrug DiscoveryInfectious DiseasesMedical Laboratory TechnologyMolecular BiologySDG 3 - Good Health and Well-beingSDG 9 - Industry, Innovation, and InfrastructureFunding Information: This research was funded by Global Health and Tropical Medicine: Grant number IHMT-UID/multi/04413/2013 and Grant number PTDC/CVT-CVT/28908/2017, FCT-Portugal.We would like to thank João Aristeu da Rosa and Aline Rimodi Rimeiro at UNESP (Universidade Estadual de São Paulo) Araraquara (Brazil) for offering four different strains of T. cruzi. JWFO and MFAS give thanks to their financial support (PhD and post-doctoral fellowships) provided by Capes/Brazil; MSS, AASJ, and HAOR give thanks to CNPq/Brazil for the Research Grant (Bolsa de Produtividade). We would also like to thank the Department of Materials Engineering at UFRN for affording us the use of their scanning electron microscope, and the Department of Biochemistry at UFRN for affording us the use of their culture room. We thank Laysa Ohana for reviewing and editing the English version of the manuscript. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Trypanosoma cruzi is a protozoan parasite responsible for Chagas disease, which affects millions around the world and is not treatable in its chronic stage. Sodium diethyldithiocarbamate is a compound belonging to the carbamate class and, in a previous study, demonstrated high efficacy against T. cruzi, showing itself to be a promising compound for the treatment of Chagas disease. This study investigates the encapsulation of sodium diethyldithiocarbamate by poly-lactic acid in nanoparticles, a system of biodegradable nanoparticles that is capable of reducing the toxicity caused by free DETC against cells and maintaining the antiparasitic activity. The nanosystem PLA-DETC was fabricated using nanoprecipitation, and its physical characterization was measured via DLS, SEM, and AFM, demonstrating a small size around 168 nm and a zeta potential of around −19 mv. Furthermore, the toxicity was determined by MTT reduction against three cell lines (VERO, 3T3, and RAW), and when compared to free DETC, we observed a reduction in cell mortality, demonstrating the importance of DETC nanoencapsulation. In addition, the nanoparticles were stained with FITC and put in contact with cells for 24 h, followed by confirmation of whether the nanosystem was inside the cells. Lastly, the antiparasitic activity against different strains of T. cruzi in trypomastigote forms was determined by resazurin reduction and ROS production, which demonstrated high efficacy towards T. cruzi equal to that of free DETC.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)RUNde Freitas Oliveira, Johny Wysllasda Silva, Mariana Farias AlvesDamasceno, Igor ZumbaRocha, Hugo Alexandre Oliveirada Silva Júnior, Arnóbio AntônioSilva, Marcelo Sousa2023-01-19T22:19:33Z2022-032022-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147867eng1999-4923PURE: 50923872https://doi.org/10.3390/pharmaceutics14030497info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:29:09Zoai:run.unl.pt:10362/147867Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:06.979169Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi
title In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi
spellingShingle In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi
de Freitas Oliveira, Johny Wysllas
Chagas disease
Nanoparticle
Nanoprecipitation
Sodium diethyldithiocarbamate
Trypanosoma cruzi
QR Microbiology
QD Chemistry
Pharmaceutical Science
Parasitology
Drug Discovery
Infectious Diseases
Medical Laboratory Technology
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 9 - Industry, Innovation, and Infrastructure
title_short In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi
title_full In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi
title_fullStr In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi
title_full_unstemmed In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi
title_sort In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against Trypanosoma cruzi
author de Freitas Oliveira, Johny Wysllas
author_facet de Freitas Oliveira, Johny Wysllas
da Silva, Mariana Farias Alves
Damasceno, Igor Zumba
Rocha, Hugo Alexandre Oliveira
da Silva Júnior, Arnóbio Antônio
Silva, Marcelo Sousa
author_role author
author2 da Silva, Mariana Farias Alves
Damasceno, Igor Zumba
Rocha, Hugo Alexandre Oliveira
da Silva Júnior, Arnóbio Antônio
Silva, Marcelo Sousa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv de Freitas Oliveira, Johny Wysllas
da Silva, Mariana Farias Alves
Damasceno, Igor Zumba
Rocha, Hugo Alexandre Oliveira
da Silva Júnior, Arnóbio Antônio
Silva, Marcelo Sousa
dc.subject.por.fl_str_mv Chagas disease
Nanoparticle
Nanoprecipitation
Sodium diethyldithiocarbamate
Trypanosoma cruzi
QR Microbiology
QD Chemistry
Pharmaceutical Science
Parasitology
Drug Discovery
Infectious Diseases
Medical Laboratory Technology
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 9 - Industry, Innovation, and Infrastructure
topic Chagas disease
Nanoparticle
Nanoprecipitation
Sodium diethyldithiocarbamate
Trypanosoma cruzi
QR Microbiology
QD Chemistry
Pharmaceutical Science
Parasitology
Drug Discovery
Infectious Diseases
Medical Laboratory Technology
Molecular Biology
SDG 3 - Good Health and Well-being
SDG 9 - Industry, Innovation, and Infrastructure
description Funding Information: This research was funded by Global Health and Tropical Medicine: Grant number IHMT-UID/multi/04413/2013 and Grant number PTDC/CVT-CVT/28908/2017, FCT-Portugal.We would like to thank João Aristeu da Rosa and Aline Rimodi Rimeiro at UNESP (Universidade Estadual de São Paulo) Araraquara (Brazil) for offering four different strains of T. cruzi. JWFO and MFAS give thanks to their financial support (PhD and post-doctoral fellowships) provided by Capes/Brazil; MSS, AASJ, and HAOR give thanks to CNPq/Brazil for the Research Grant (Bolsa de Produtividade). We would also like to thank the Department of Materials Engineering at UFRN for affording us the use of their scanning electron microscope, and the Department of Biochemistry at UFRN for affording us the use of their culture room. We thank Laysa Ohana for reviewing and editing the English version of the manuscript. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-03
2022-03-01T00:00:00Z
2023-01-19T22:19:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/147867
url http://hdl.handle.net/10362/147867
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1999-4923
PURE: 50923872
https://doi.org/10.3390/pharmaceutics14030497
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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